Bin Li , Junnan Ding , Xiaoqian Liu , Dan Zhu , Mengmeng Zhang , Lina Qu , Wei Li , Yue Wang , Xin Li
{"title":"退耕还林还草促进中国东北地区土壤质量恢复来自土壤氮循环的证据","authors":"Bin Li , Junnan Ding , Xiaoqian Liu , Dan Zhu , Mengmeng Zhang , Lina Qu , Wei Li , Yue Wang , Xin Li","doi":"10.1016/j.apsoil.2024.105655","DOIUrl":null,"url":null,"abstract":"<div><div>Soils contain a diversity of microbial communities involved in the nitrogen cycle (N-cycling), which are essential for maintaining the quality and health of soil. However, agricultural intensification has led to land use conversion, which has had a negative impact on ecosystems. There is a lack of research on the whole N-cycling process in land use change under the context of ecological restoration. Here, we utilized qPCR (Quantitative Real-time PCR) and MiSeq techniques to analyse the functional genes related to nitrogen fixation (<em>nifH</em>), nitrification (AOA and AOB) and denitrification (<em>norB</em> and <em>nosZ</em>) to explore whether the conversion of farmland to forest and grassland affects changes in microbial communities involved in the N-cycling. We reveal the potential effects of ecological restoration on microbial community function in the N-cycling through soil properties. Specifically, land use conversion increased N-cycling gene abundance and changed in the N-cycling microbiome exhibited. The ecological restoration has increased the importance of stochastic processes in the assembly of AOA and AOB communities and affected the ecological cluster of soil N-cycling microbial network. Overall, these findings reveal specific microbial processes and interactions that influence the soil N-cycling and provide insights for future research on sustainable land management approaches that support soil health.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"203 ","pages":"Article 105655"},"PeriodicalIF":4.8000,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Returning farmland to forests and grasslands promotes soil quality restoration in Northeast China: Evidence from soil nitrogen cycle\",\"authors\":\"Bin Li , Junnan Ding , Xiaoqian Liu , Dan Zhu , Mengmeng Zhang , Lina Qu , Wei Li , Yue Wang , Xin Li\",\"doi\":\"10.1016/j.apsoil.2024.105655\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Soils contain a diversity of microbial communities involved in the nitrogen cycle (N-cycling), which are essential for maintaining the quality and health of soil. However, agricultural intensification has led to land use conversion, which has had a negative impact on ecosystems. There is a lack of research on the whole N-cycling process in land use change under the context of ecological restoration. Here, we utilized qPCR (Quantitative Real-time PCR) and MiSeq techniques to analyse the functional genes related to nitrogen fixation (<em>nifH</em>), nitrification (AOA and AOB) and denitrification (<em>norB</em> and <em>nosZ</em>) to explore whether the conversion of farmland to forest and grassland affects changes in microbial communities involved in the N-cycling. We reveal the potential effects of ecological restoration on microbial community function in the N-cycling through soil properties. Specifically, land use conversion increased N-cycling gene abundance and changed in the N-cycling microbiome exhibited. The ecological restoration has increased the importance of stochastic processes in the assembly of AOA and AOB communities and affected the ecological cluster of soil N-cycling microbial network. Overall, these findings reveal specific microbial processes and interactions that influence the soil N-cycling and provide insights for future research on sustainable land management approaches that support soil health.</div></div>\",\"PeriodicalId\":8099,\"journal\":{\"name\":\"Applied Soil Ecology\",\"volume\":\"203 \",\"pages\":\"Article 105655\"},\"PeriodicalIF\":4.8000,\"publicationDate\":\"2024-10-05\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Soil Ecology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S092913932400386X\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"SOIL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Soil Ecology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S092913932400386X","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}
Returning farmland to forests and grasslands promotes soil quality restoration in Northeast China: Evidence from soil nitrogen cycle
Soils contain a diversity of microbial communities involved in the nitrogen cycle (N-cycling), which are essential for maintaining the quality and health of soil. However, agricultural intensification has led to land use conversion, which has had a negative impact on ecosystems. There is a lack of research on the whole N-cycling process in land use change under the context of ecological restoration. Here, we utilized qPCR (Quantitative Real-time PCR) and MiSeq techniques to analyse the functional genes related to nitrogen fixation (nifH), nitrification (AOA and AOB) and denitrification (norB and nosZ) to explore whether the conversion of farmland to forest and grassland affects changes in microbial communities involved in the N-cycling. We reveal the potential effects of ecological restoration on microbial community function in the N-cycling through soil properties. Specifically, land use conversion increased N-cycling gene abundance and changed in the N-cycling microbiome exhibited. The ecological restoration has increased the importance of stochastic processes in the assembly of AOA and AOB communities and affected the ecological cluster of soil N-cycling microbial network. Overall, these findings reveal specific microbial processes and interactions that influence the soil N-cycling and provide insights for future research on sustainable land management approaches that support soil health.
期刊介绍:
Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.