{"title":"微流控技术辅助从苕子叶水提取物中绿色合成氧化铜纳米颗粒及其抗菌和抗氧化活性","authors":"D. Joanna Magdalene, D. Muthuselvam","doi":"10.1016/j.hermed.2024.100940","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Green nanosynthesis of medicinal plants are useful for the development of antibiotic agents. In this work, we have explored the antimicrobial and antioxidant activity of green synthesised copper oxide (CuO) nanoparticles (NPs) from tropical medicinal plant <em>Ipomoea quamoclit</em> L.</div></div><div><h3>Methods</h3><div>With the aid of microfluidic technology, a low-cost, polydimethylsiloxane (PDMS) lab-on-chip micromixer for green nanosynthesis of CuO nanoparticle using <em>Ipomoea quamoclit</em> is presented. The UV–Vis characteristics, Fourier Transform Infra-red (FTIR) spectrum, antimicrobial and antioxidant response using DPPH and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) were reported. The antimicrobial efficacy of the synthesised CuO-NPs is tested against two-gram positive bacteria <em>Staphylococcus aureus, Micrococcus luteus,</em> two gram negative bacteria, <em>Enterobacter cloacae</em>, <em>Klebsiella pneumoniae</em> and two fungi species <em>Candida albicans</em>, <em>Aspergillus niger.</em></div></div><div><h3>Results</h3><div>UV–Vis spectrum for the synthesised CuO-NPs showed the absorbance at 670 nm, scanning electron microscope (SEM) image analysis and dynamic light scattering (DLS) analysis confirmed the presence of spherical shaped nanoparticle sized between 65 and 94.5 nm. The presence of bioactive functional groups of N-H, C-H and C=0 stretching was identified using Fourier Transform Infrared spectroscopy (FTIR). The maximum mean zone of inhibition obtained was 10.45<!--> <!-->±<!--> <!-->0.25 mm for fungi <em>A. niger</em>. The antioxidant activity using DPPH scavenging assay and hydrogen peroxide scavenging assay showed the IC<sub>50</sub> value 33.94 μg/ml and 42.54 μg/ml respectively.</div></div><div><h3>Conclusions</h3><div>The obtained results reveal the potential of CuO-NPs synthesised from <em>I. quamoclit</em> L. as a remarkable medicinal entity for wound healing, respiratory infection, fungal infection and urinary tract infection along with the improved antimicrobial and antioxidant activity.</div></div>","PeriodicalId":56077,"journal":{"name":"Journal of Herbal Medicine","volume":"48 ","pages":"Article 100940"},"PeriodicalIF":2.2000,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Microfluidics assisted green synthesis of copper oxide nanoparticles from the aqueous leaf extract of Ipomoea quamoclit L. with its antimicrobial and antioxidant activity\",\"authors\":\"D. Joanna Magdalene, D. Muthuselvam\",\"doi\":\"10.1016/j.hermed.2024.100940\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Introduction</h3><div>Green nanosynthesis of medicinal plants are useful for the development of antibiotic agents. In this work, we have explored the antimicrobial and antioxidant activity of green synthesised copper oxide (CuO) nanoparticles (NPs) from tropical medicinal plant <em>Ipomoea quamoclit</em> L.</div></div><div><h3>Methods</h3><div>With the aid of microfluidic technology, a low-cost, polydimethylsiloxane (PDMS) lab-on-chip micromixer for green nanosynthesis of CuO nanoparticle using <em>Ipomoea quamoclit</em> is presented. The UV–Vis characteristics, Fourier Transform Infra-red (FTIR) spectrum, antimicrobial and antioxidant response using DPPH and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) were reported. The antimicrobial efficacy of the synthesised CuO-NPs is tested against two-gram positive bacteria <em>Staphylococcus aureus, Micrococcus luteus,</em> two gram negative bacteria, <em>Enterobacter cloacae</em>, <em>Klebsiella pneumoniae</em> and two fungi species <em>Candida albicans</em>, <em>Aspergillus niger.</em></div></div><div><h3>Results</h3><div>UV–Vis spectrum for the synthesised CuO-NPs showed the absorbance at 670 nm, scanning electron microscope (SEM) image analysis and dynamic light scattering (DLS) analysis confirmed the presence of spherical shaped nanoparticle sized between 65 and 94.5 nm. The presence of bioactive functional groups of N-H, C-H and C=0 stretching was identified using Fourier Transform Infrared spectroscopy (FTIR). The maximum mean zone of inhibition obtained was 10.45<!--> <!-->±<!--> <!-->0.25 mm for fungi <em>A. niger</em>. The antioxidant activity using DPPH scavenging assay and hydrogen peroxide scavenging assay showed the IC<sub>50</sub> value 33.94 μg/ml and 42.54 μg/ml respectively.</div></div><div><h3>Conclusions</h3><div>The obtained results reveal the potential of CuO-NPs synthesised from <em>I. quamoclit</em> L. as a remarkable medicinal entity for wound healing, respiratory infection, fungal infection and urinary tract infection along with the improved antimicrobial and antioxidant activity.</div></div>\",\"PeriodicalId\":56077,\"journal\":{\"name\":\"Journal of Herbal Medicine\",\"volume\":\"48 \",\"pages\":\"Article 100940\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-09-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Herbal Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2210803324000976\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Herbal Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2210803324000976","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
Microfluidics assisted green synthesis of copper oxide nanoparticles from the aqueous leaf extract of Ipomoea quamoclit L. with its antimicrobial and antioxidant activity
Introduction
Green nanosynthesis of medicinal plants are useful for the development of antibiotic agents. In this work, we have explored the antimicrobial and antioxidant activity of green synthesised copper oxide (CuO) nanoparticles (NPs) from tropical medicinal plant Ipomoea quamoclit L.
Methods
With the aid of microfluidic technology, a low-cost, polydimethylsiloxane (PDMS) lab-on-chip micromixer for green nanosynthesis of CuO nanoparticle using Ipomoea quamoclit is presented. The UV–Vis characteristics, Fourier Transform Infra-red (FTIR) spectrum, antimicrobial and antioxidant response using DPPH and hydrogen peroxide (H2O2) were reported. The antimicrobial efficacy of the synthesised CuO-NPs is tested against two-gram positive bacteria Staphylococcus aureus, Micrococcus luteus, two gram negative bacteria, Enterobacter cloacae, Klebsiella pneumoniae and two fungi species Candida albicans, Aspergillus niger.
Results
UV–Vis spectrum for the synthesised CuO-NPs showed the absorbance at 670 nm, scanning electron microscope (SEM) image analysis and dynamic light scattering (DLS) analysis confirmed the presence of spherical shaped nanoparticle sized between 65 and 94.5 nm. The presence of bioactive functional groups of N-H, C-H and C=0 stretching was identified using Fourier Transform Infrared spectroscopy (FTIR). The maximum mean zone of inhibition obtained was 10.45 ± 0.25 mm for fungi A. niger. The antioxidant activity using DPPH scavenging assay and hydrogen peroxide scavenging assay showed the IC50 value 33.94 μg/ml and 42.54 μg/ml respectively.
Conclusions
The obtained results reveal the potential of CuO-NPs synthesised from I. quamoclit L. as a remarkable medicinal entity for wound healing, respiratory infection, fungal infection and urinary tract infection along with the improved antimicrobial and antioxidant activity.
期刊介绍:
The Journal of Herbal Medicine, the official journal of the National Institute of Medical Herbalists, is a peer reviewed journal which aims to serve its readers as an authoritative resource on the profession and practice of herbal medicine. The content areas of the journal reflect the interests of Medical Herbalists and other health professionals interested in the clinical and professional application of botanical medicines. The objective is to strengthen the research and educational base of herbal medicine with research papers in the form of case studies, original research articles and reviews, monographs, clinical trials and relevant in vitro studies. It also publishes policy statements, opinion pieces, book reviews, conference proceedings and profession related information such as pharmacovigilance reports providing an information source for not only the Herbal Practitioner but any Health professional with an interest in phytotherapy.