Developing a highly efficient fluorescence strategy for thiamine detection in real samples

This research presents the first report of a simple fluorescent approach for determination of thiamine (TH, Vitamin B1) with great sensitivity and selectivity by manganese dioxide (MnO₂) nanosheets. These nanoparticles have several advantages such as activity of oxidase like and easy, low-cost preparation. They can successfully oxidize nonfluorescent TH to intense fluorescence thiochrome at physiological pH and room temperature. Under optimized conditions and in the presence of EDTA and HCl, a linear relationship was gained between fluorescence intensities and thiamine concentrations from 10 to 5000 nM. This fluorometric method demonstrated high sensitivity with a low detection limit (5 nM). In order to confirm the selectivity of the proposed system, related interfering agents were investigated with the MnO₂ nanosheets and it was verified that the fluorescence response is greatly selective to TH. This method exhibited acceptable outcomes to evaluate TH in pharmaceutical, food, urine and serum specimens. Therefore, we suggest that the current study could be appropriate for TH measurement in biological, food and drug samples.