环境相关浓度的土霉素在嗜水气单胞菌和塔氏爱德华氏菌产生抗菌药耐药性中的作用

IF 6.6 Q1 ENGINEERING, ENVIRONMENTAL
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引用次数: 0

摘要

该研究评估了体外土霉素(OTC)暴露对水生细菌抗菌素耐药性(AMR)发展的影响。将嗜水气单胞菌 ATCC 49140 和 Edwardsiella tarda ATCC 15947 分序列置于 OTC 的短期和长期暴露中。在 0、0.097(0.25 倍最低抑菌浓度:MIC)、0.195(0.5 倍 MIC)和 0.39 µg mL-1(1 倍 MIC)的条件下,短时间接触 OTC(30 分钟)会引起嗜水气单胞菌(10-4)和 Edwardsiella tarda(10-2)的高突变率。连续暴露于抑制浓度的 OTC(0.39 微克/毫升-1)可将硝基呋喃妥因(NIT)对蚜虫的抑制区以及四环素、NIT 和庆大霉素对 E. tarda 的抑制区缩小到具有临床意义的水平。在连续接触 0.097、0.195 和 0.39 微克毫升-1 OTC 的过程中,蚜虫和 E. tarda 的 OTC MICs 分别上升了 4 倍(1.56 微克毫升-1)和 8 倍(3.12 微克毫升-1)。暴露于 OTC 还会使杀菌剂三氯生(TCS)在蛛水蚤中的中等有效浓度增加 4 倍(100 微克/毫升-1),但没有任何浓度依赖性模式。这项研究表明,环境中的 OTC 含量会导致细菌变异和 AMR,从而可能导致对 TCS 等杀菌剂产生交叉耐药性。减少 OTC 的使用并改进处置方法有助于通过更好的抗菌剂管理来限制 AMR 的传播。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of environmentally relevant concentrations of oxytetracycline in the emergence of antimicrobial resistance in Aeromonas hydrophila and Edwardsiella tarda
The study assessed the efficiency of in-vitro oxytetracycline (OTC) exposure on antimicrobial resistance (AMR) development in aquatic bacteria. Aeromonas hydrophila ATCC 49140 and Edwardsiella tarda ATCC 15947 were serially passaged to OTC for short and long durations. Short duration OTC exposure (30 min) at 0, 0.097 (0.25× Minimum Inhibitory Concentration: MIC), 0.195 (0.5× MIC) and 0.39 µg mL−1 (1× MIC) elicited high mutation rate in A. hydrophila (10−4) and E. tarda (10−2). Serial passage exposure to inhibitory concentration of OTC (0.39 µg mL−1) reduced the zone of inhibition of nitrofurantoin (NIT) for A. hydrophila and of tetracycline, NIT and gentamicin for E. tarda to a clinically significant level. The upsurge in MICs of OTC was 4-folds (1.56 µg mL−1) and 8-folds (3.12 µg mL−1) in A. hydrophila and E. tarda, respectively during serial passage exposure to 0.097, 0.195 and 0.39 µg mL−1 OTC. OTC exposure also increased the MICs of biocide triclosan (TCS) >4-folds (>100 µg mL−1) in A. hydrophila without any concentration-dependent pattern. This study shows that environmental OTC levels can drive bacterial mutation and AMR, potentially leading to cross-resistance to biocides like TCS. Reducing OTC use and improving disposal could help limit AMR spread through better antimicrobial management.
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来源期刊
Journal of hazardous materials letters
Journal of hazardous materials letters Pollution, Health, Toxicology and Mutagenesis, Environmental Chemistry, Waste Management and Disposal, Environmental Engineering
CiteScore
10.30
自引率
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审稿时长
20 days
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