使用标准化行为测定法研究斑马鱼幼虫期光/暗运动的规程。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-10-10 DOI:10.1016/j.xpro.2024.103346

Courtney Hillman, James Kearn, Matthew O Parker

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引用次数: 0

摘要

斑马鱼（Danio rerio）幼体为高通量体内研究提供了一个独特的途径。光/暗运动试验被广泛使用，但缺乏实验一致性。在此，我们介绍了一种标准化的光/暗试验方案，描述了培养、驯化幼虫、进行试验和制备药物暴露溶液的步骤。我们还详细介绍了药物暴露和数据分析的程序。有关使用和执行该方案的完整细节，请参阅 Hillman 等人的文章1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Protocol for investigating light/dark locomotion in larval stage zebrafish using a standardized behavioral assay.

Zebrafish (Danio rerio) larvae offer a unique avenue for high-throughput in vivo investigation. The light/dark locomotor assay is widely used but lacks experimental consistency. Here, we present a protocol for a standardized light/dark assay by describing the steps for plating, acclimatizing larvae, performing the assay, and preparing drug exposure solutions. We also detail procedures for substance exposure and data analysis. For complete details on the use and execution of this protocol, please refer to Hillman et al.¹.

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