通过配对形态学和单细胞RNA-seq分析验证基于非破坏性形态学的大脑皮质有器官选择。

IF 5.9 2区 医学 Q1 CELL & TISSUE ENGINEERING
Stem Cell Reports Pub Date : 2024-11-12 Epub Date: 2024-10-10 DOI:10.1016/j.stemcr.2024.09.005
Megumi Ikeda, Daisuke Doi, Hayao Ebise, Yuki Ozaki, Misaki Fujii, Tetsuhiro Kikuchi, Kenji Yoshida, Jun Takahashi
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引用次数: 0

摘要

有机体是一种自组织细胞聚集体,对开发疾病模型和基于细胞的疗法有重大贡献。然而,尽管使用了最新的分化方案,类器官之间的差异仍不可避免。在这里,我们重点研究了在大脑类器官分化培养中形成的类器官的形态,并通过单细胞RNA测序分析评估了它们的细胞组成。数据显示,主要由神经嵴和脉络丛等非神经元细胞组成的类器官显示出独特的形态特征。此外,我们还证明了非破坏性形态分析可以准确区分由大脑皮质组织和其他脑组织组成的类器官,从而提高实验的准确性和可靠性,确保细胞疗法的安全性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Validation of non-destructive morphology-based selection of cerebral cortical organoids by paired morphological and single-cell RNA-seq analyses.

Organoids, self-organized cell aggregates, contribute significantly to developing disease models and cell-based therapies. Organoid-to-organoid variations, however, are inevitable despite the use of the latest differentiation protocols. Here, we focused on the morphology of organoids formed in a cerebral organoid differentiation culture and assessed their cellular compositions by single-cell RNA sequencing analysis. The data revealed that organoids primarily composed of non-neuronal cells, such as those from the neural crest and choroid plexus, showed unique morphological features. Moreover, we demonstrate that non-destructive morphological analysis can accurately distinguish organoids composed of cerebral cortical tissues from other cerebral tissues, thus enhancing experimental accuracy and reliability to ensure the safety of cell-based therapies.

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来源期刊
Stem Cell Reports
Stem Cell Reports CELL & TISSUE ENGINEERING-CELL BIOLOGY
CiteScore
10.50
自引率
1.70%
发文量
200
审稿时长
28 weeks
期刊介绍: Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.
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