Maoxing Zhang , Boyang Zhou , Yuan Cheng , Chunyan Zhong , Min Yu , Ting Pan , Yiyong Zhu
{"title":"质膜 H+-ATP 酶参与籼稻对硝酸盐营养的吸收和利用","authors":"Maoxing Zhang , Boyang Zhou , Yuan Cheng , Chunyan Zhong , Min Yu , Ting Pan , Yiyong Zhu","doi":"10.1016/j.jplph.2024.154368","DOIUrl":null,"url":null,"abstract":"<div><div>Utilization of nitrogen by crops is essential for sustainable agriculture. The transport of nitrate (NO<sub>3</sub><sup>−</sup>) across the plasma membrane is a critical gateway for N uptake and subsequent utilization. This process requires proton (H<sup>+</sup>) coupled cotransport, which is driven by proton motive force, provided by plasma membrane (PM) H<sup>+</sup>-ATPase. In this report, two indica rice varieties [Meixiangzhan 2 (MXZ) and Jifengyou 1002 (JFY)] in South China were selected and cultivated in hydroponic solution with 0.5 mM or 2.0 mM NO<sub>3</sub><sup>−</sup> as the N source. The JFY exhibited stronger growth with higher biomass than MXZ under both 0.5 mM and 2.0 mM NO<sub>3</sub><sup>−</sup>. PM H<sup>+</sup>-ATPase activity of JFY roots was significantly higher than that of MXZ. The higher PM H<sup>+</sup>-ATPase activity in JFY was consistent with a higher abundance of PM H<sup>+</sup>-ATPase protein and higher transcription levels of <em>OSAs</em>, such as <em>OSA2</em>, <em>OSA7</em> and <em>OSA8</em> in roots, <em>OSA3</em>, <em>OSA7</em> and <em>OSA8</em> in leaves. The expression of nitrate transporters (<em>OsNRT1;1b</em>, <em>OsNRT2.1</em>, <em>OsNRT2.2</em>, and <em>OsNAR2.1</em>) were also higher in roots or shoots of JFY than those in MXZ. Under 0.5 mM and 2.0 mM NO<sub>3</sub><sup>−</sup>, the NO<sub>3</sub><sup>−</sup> absorption and translocation rate, nitrate content, as well as nitrate reductase (NR) activity were all significantly higher in JFY, as compared to those in MXZ. Taken together, in JFY and MXZ, a higher level of PM H<sup>+</sup>-ATPase protein and higher activity coupled with greater efficiency in nitrate uptake, translocation and assimilation, suggesting the existence of a close correlation between PM H<sup>+</sup>-ATPase and nitrate utilization in indica rice. PM H<sup>+</sup>-ATPase may one of the elite genes that can contribute to nitrate use efficiency in rice.</div></div>","PeriodicalId":16808,"journal":{"name":"Journal of plant physiology","volume":"303 ","pages":"Article 154368"},"PeriodicalIF":4.0000,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Involvement of plasma membrane H+-ATPase in the nitrate-nutrition uptake and utilization in indica rice\",\"authors\":\"Maoxing Zhang , Boyang Zhou , Yuan Cheng , Chunyan Zhong , Min Yu , Ting Pan , Yiyong Zhu\",\"doi\":\"10.1016/j.jplph.2024.154368\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Utilization of nitrogen by crops is essential for sustainable agriculture. The transport of nitrate (NO<sub>3</sub><sup>−</sup>) across the plasma membrane is a critical gateway for N uptake and subsequent utilization. This process requires proton (H<sup>+</sup>) coupled cotransport, which is driven by proton motive force, provided by plasma membrane (PM) H<sup>+</sup>-ATPase. In this report, two indica rice varieties [Meixiangzhan 2 (MXZ) and Jifengyou 1002 (JFY)] in South China were selected and cultivated in hydroponic solution with 0.5 mM or 2.0 mM NO<sub>3</sub><sup>−</sup> as the N source. The JFY exhibited stronger growth with higher biomass than MXZ under both 0.5 mM and 2.0 mM NO<sub>3</sub><sup>−</sup>. PM H<sup>+</sup>-ATPase activity of JFY roots was significantly higher than that of MXZ. The higher PM H<sup>+</sup>-ATPase activity in JFY was consistent with a higher abundance of PM H<sup>+</sup>-ATPase protein and higher transcription levels of <em>OSAs</em>, such as <em>OSA2</em>, <em>OSA7</em> and <em>OSA8</em> in roots, <em>OSA3</em>, <em>OSA7</em> and <em>OSA8</em> in leaves. The expression of nitrate transporters (<em>OsNRT1;1b</em>, <em>OsNRT2.1</em>, <em>OsNRT2.2</em>, and <em>OsNAR2.1</em>) were also higher in roots or shoots of JFY than those in MXZ. Under 0.5 mM and 2.0 mM NO<sub>3</sub><sup>−</sup>, the NO<sub>3</sub><sup>−</sup> absorption and translocation rate, nitrate content, as well as nitrate reductase (NR) activity were all significantly higher in JFY, as compared to those in MXZ. Taken together, in JFY and MXZ, a higher level of PM H<sup>+</sup>-ATPase protein and higher activity coupled with greater efficiency in nitrate uptake, translocation and assimilation, suggesting the existence of a close correlation between PM H<sup>+</sup>-ATPase and nitrate utilization in indica rice. PM H<sup>+</sup>-ATPase may one of the elite genes that can contribute to nitrate use efficiency in rice.</div></div>\",\"PeriodicalId\":16808,\"journal\":{\"name\":\"Journal of plant physiology\",\"volume\":\"303 \",\"pages\":\"Article 154368\"},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-10-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of plant physiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0176161724001998\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of plant physiology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0176161724001998","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Involvement of plasma membrane H+-ATPase in the nitrate-nutrition uptake and utilization in indica rice
Utilization of nitrogen by crops is essential for sustainable agriculture. The transport of nitrate (NO3−) across the plasma membrane is a critical gateway for N uptake and subsequent utilization. This process requires proton (H+) coupled cotransport, which is driven by proton motive force, provided by plasma membrane (PM) H+-ATPase. In this report, two indica rice varieties [Meixiangzhan 2 (MXZ) and Jifengyou 1002 (JFY)] in South China were selected and cultivated in hydroponic solution with 0.5 mM or 2.0 mM NO3− as the N source. The JFY exhibited stronger growth with higher biomass than MXZ under both 0.5 mM and 2.0 mM NO3−. PM H+-ATPase activity of JFY roots was significantly higher than that of MXZ. The higher PM H+-ATPase activity in JFY was consistent with a higher abundance of PM H+-ATPase protein and higher transcription levels of OSAs, such as OSA2, OSA7 and OSA8 in roots, OSA3, OSA7 and OSA8 in leaves. The expression of nitrate transporters (OsNRT1;1b, OsNRT2.1, OsNRT2.2, and OsNAR2.1) were also higher in roots or shoots of JFY than those in MXZ. Under 0.5 mM and 2.0 mM NO3−, the NO3− absorption and translocation rate, nitrate content, as well as nitrate reductase (NR) activity were all significantly higher in JFY, as compared to those in MXZ. Taken together, in JFY and MXZ, a higher level of PM H+-ATPase protein and higher activity coupled with greater efficiency in nitrate uptake, translocation and assimilation, suggesting the existence of a close correlation between PM H+-ATPase and nitrate utilization in indica rice. PM H+-ATPase may one of the elite genes that can contribute to nitrate use efficiency in rice.
期刊介绍:
The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication.
The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.