质膜 H+-ATP 酶参与籼稻对硝酸盐营养的吸收和利用

IF 4 3区 生物学 Q1 PLANT SCIENCES
Maoxing Zhang , Boyang Zhou , Yuan Cheng , Chunyan Zhong , Min Yu , Ting Pan , Yiyong Zhu
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引用次数: 0

摘要

作物对氮的利用对于可持续农业至关重要。硝酸盐(NO3-)跨质膜运输是氮吸收和随后利用的关键途径。这一过程需要质子(H+)耦合共转运,由质膜(PM)H+-ATP 酶提供的质子动力驱动。本报告选取了中国南方的两个籼稻品种[梅香占 2 号(MXZ)和吉丰优 1002(JFY)],在以 0.5 mM 或 2.0 mM NO3- 为氮源的水培溶液中进行栽培。在 0.5 mM 和 2.0 mM NO3- 条件下,JFY 都比 MXZ 生长更强,生物量更高。JFY 根的 PM H+-ATPase 活性明显高于 MXZ。JFY 的 PM H+-ATPase 活性较高,这与根中较高的 PM H+-ATPase 蛋白丰度和较高的 OSAs(如 OSA2、OSA7 和 OSA8,叶中的 OSA3、OSA7 和 OSA8)转录水平一致。硝酸盐转运体(OsNRT1;1b、OsNRT2.1、OsNRT2.2 和 OsNAR2.1)在 JFY 根或芽中的表达量也高于 MXZ。在 0.5 mM 和 2.0 mM NO3-条件下,JFY 的 NO3-吸收和转运率、硝酸盐含量以及硝酸还原酶(NR)活性均显著高于 MXZ。综上所述,JFY 和 MXZ 中 PM H+-ATPase 蛋白水平更高、活性更高,硝酸盐的吸收、转运和同化效率也更高,表明籼稻 PM H+-ATPase 与硝酸盐利用之间存在密切的相关性。PM H+-ATPase 可能是有助于提高水稻硝酸盐利用效率的精英基因之一。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Involvement of plasma membrane H+-ATPase in the nitrate-nutrition uptake and utilization in indica rice
Utilization of nitrogen by crops is essential for sustainable agriculture. The transport of nitrate (NO3) across the plasma membrane is a critical gateway for N uptake and subsequent utilization. This process requires proton (H+) coupled cotransport, which is driven by proton motive force, provided by plasma membrane (PM) H+-ATPase. In this report, two indica rice varieties [Meixiangzhan 2 (MXZ) and Jifengyou 1002 (JFY)] in South China were selected and cultivated in hydroponic solution with 0.5 mM or 2.0 mM NO3 as the N source. The JFY exhibited stronger growth with higher biomass than MXZ under both 0.5 mM and 2.0 mM NO3. PM H+-ATPase activity of JFY roots was significantly higher than that of MXZ. The higher PM H+-ATPase activity in JFY was consistent with a higher abundance of PM H+-ATPase protein and higher transcription levels of OSAs, such as OSA2, OSA7 and OSA8 in roots, OSA3, OSA7 and OSA8 in leaves. The expression of nitrate transporters (OsNRT1;1b, OsNRT2.1, OsNRT2.2, and OsNAR2.1) were also higher in roots or shoots of JFY than those in MXZ. Under 0.5 mM and 2.0 mM NO3, the NO3 absorption and translocation rate, nitrate content, as well as nitrate reductase (NR) activity were all significantly higher in JFY, as compared to those in MXZ. Taken together, in JFY and MXZ, a higher level of PM H+-ATPase protein and higher activity coupled with greater efficiency in nitrate uptake, translocation and assimilation, suggesting the existence of a close correlation between PM H+-ATPase and nitrate utilization in indica rice. PM H+-ATPase may one of the elite genes that can contribute to nitrate use efficiency in rice.
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来源期刊
Journal of plant physiology
Journal of plant physiology 生物-植物科学
CiteScore
7.20
自引率
4.70%
发文量
196
审稿时长
32 days
期刊介绍: The Journal of Plant Physiology is a broad-spectrum journal that welcomes high-quality submissions in all major areas of plant physiology, including plant biochemistry, functional biotechnology, computational and synthetic plant biology, growth and development, photosynthesis and respiration, transport and translocation, plant-microbe interactions, biotic and abiotic stress. Studies are welcome at all levels of integration ranging from molecules and cells to organisms and their environments and are expected to use state-of-the-art methodologies. Pure gene expression studies are not within the focus of our journal. To be considered for publication, papers must significantly contribute to the mechanistic understanding of physiological processes, and not be merely descriptive, or confirmatory of previous results. We encourage the submission of papers that explore the physiology of non-model as well as accepted model species and those that bridge basic and applied research. For instance, studies on agricultural plants that show new physiological mechanisms to improve agricultural efficiency are welcome. Studies performed under uncontrolled situations (e.g. field conditions) not providing mechanistic insight will not be considered for publication. The Journal of Plant Physiology publishes several types of articles: Original Research Articles, Reviews, Perspectives Articles, and Short Communications. Reviews and Perspectives will be solicited by the Editors; unsolicited reviews are also welcome but only from authors with a strong track record in the field of the review. Original research papers comprise the majority of published contributions.
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