SRF 促进长程染色质环的形成和干细胞的多能性。

IF 7.5 1区生物学 Q1 CELL BIOLOGY

Cell reports Pub Date : 2024-10-22 Epub Date: 2024-10-10 DOI:10.1016/j.celrep.2024.114846

Pavel Tsaytler, Gaby Blaess, Manuela Scholze-Wittler, David Meierhofer, Lars Wittler, Frederic Koch, Bernhard G Herrmann

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引用次数: 0

摘要

血清反应因子（SRF）是一种对细胞增殖、分化和迁移至关重要的转录因子，是胚胎中原始条纹和中胚层形成所必需的。SRF 的典型作用是由一系列不同的环境依赖性辅助因子介导的。在这里，我们发现 SRF 与 CTCF 和凝聚素亚基在拓扑关联域（TAD）边界和环锚处发生物理相互作用。SRF促进长程染色质环的形成，并有助于TAD的绝缘。在胚胎干细胞（ESC）中，SRF与SOX2和NANOG结合，促进了三维（3D）多能中心的形成。我们的研究结果揭示了SRF在高阶染色质组织中的其他作用。

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SRF promotes long-range chromatin loop formation and stem cell pluripotency.

Serum response factor (SRF) is a transcription factor essential for cell proliferation, differentiation, and migration and is required for primitive streak and mesoderm formation in the embryo. The canonical roles of SRF are mediated by a diverse set of context-dependent cofactors. Here, we show that SRF physically interacts with CTCF and cohesin subunits at topologically associating domain (TAD) boundaries and loop anchors. SRF promotes long-range chromatin loop formation and contributes to TAD insulation. In embryonic stem cells (ESCs), SRF associates with SOX2 and NANOG and contributes to the formation of three-dimensional (3D) pluripotency hubs. Our findings reveal additional roles of SRF in higher-order chromatin organization.

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来源期刊

Cell reports CELL BIOLOGY-

CiteScore

13.80

自引率

1.10%

发文量

1305

审稿时长

77 days

期刊介绍： Cell Reports publishes high-quality research across the life sciences and focuses on new biological insight as its primary criterion for publication. The journal offers three primary article types: Reports, which are shorter single-point articles, research articles, which are longer and provide deeper mechanistic insights, and resources, which highlight significant technical advances or major informational datasets that contribute to biological advances. Reviews covering recent literature in emerging and active fields are also accepted. The Cell Reports Portfolio includes gold open-access journals that cover life, medical, and physical sciences, and its mission is to make cutting-edge research and methodologies available to a wide readership. The journal's professional in-house editors work closely with authors, reviewers, and the scientific advisory board, which consists of current and future leaders in their respective fields. The advisory board guides the scope, content, and quality of the journal, but editorial decisions are independently made by the in-house scientific editors of Cell Reports.