由羟基脂肪酸交联的聚合苯基丙酮衍生物构成油菜孢子粉的核心结构

IF 15.8 1区 生物学 Q1 PLANT SCIENCES
Xiao Chen, Dou-Dou Huang, Jing-Shi Xue, Jiang-He Bu, Ming-Qiang Guo, Ming-Qi Zhang, Na Wang, Ji-Rong Huang, Wan-Sheng Chen, Zhong-Nan Yang
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引用次数: 0

摘要

孢子粉素是陆生植物进化过程中的一项重要创新,是陆生植物孢子和花粉外壁的核心组成部分。尽管孢粉蛋白意义重大,但由于其化学惰性极强,其基本结构仍然难以捉摸。在这项研究中,我们用乙醇胺完全溶解了油菜孢子粉素,并成功鉴定出脂肪酸、对香豆酸、甾醇和聚合苯丙氨酯衍生物等共 22 种成分。之后,我们利用 NaOH 处理和部分溶解,并结合拟南芥突变体分析和光谱方法,确定了由羟基脂肪酸交联的聚合苯丙氨酯衍生物是孢子粉素的核心结构。聚合苯丙醇衍生物的游离羟基和羧基可被其他脂肪酸(C16:0、C18:0 和 C18:3)以及醇/酚(如柚皮苷、β-谷甾醇)修饰,从而形成一种保护花粉免受陆地胁迫的结构。这一发现为进一步探索孢粉在植物繁殖和进化中的作用奠定了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Polymeric phenylpropanoid derivatives crosslinked by hydroxyl fatty acids form the core structure of rape sporopollenin

Polymeric phenylpropanoid derivatives crosslinked by hydroxyl fatty acids form the core structure of rape sporopollenin

Polymeric phenylpropanoid derivatives crosslinked by hydroxyl fatty acids form the core structure of rape sporopollenin
Sporopollenin, a critical innovation in the evolution of terrestrial plants, is the core building brick for the outer wall of land-plant spores and pollen. Despite its significance, the basic structure of sporopollenin remains elusive due to its extreme chemical inertness. In this study, we used ethanolamine to completely dissolve rape sporopollenin and successfully identified a total of 22 components, including fatty acids, p-coumaric acid, sterols and polymeric phenylpropanoid derivatives. After that, using NaOH treatment and partial dissolution, alongside Arabidopsis mutants analysis and spectroscopic methods, we determined that polymeric phenylpropanoid derivatives crosslinked by hydroxyl fatty acids serve as the core structure of sporopollenin. The free hydroxyl groups and carboxyl groups of the polymeric phenylpropanoid derivatives can be modified by other fatty acids (C16:0, C18:0 and C18:3) as well as alcohols/phenols (for example, naringenin, β-sitosterol), resulting in a structure that protects pollen from terrestrial stresses. This discovery provides a basis for further exploration of sporopollenin’s role in plant reproduction and evolution. The core structure of rape sporopollenin consists of polymeric phenylpropanoid derivatives crosslinked by hydroxyl fatty acids, while other components ligated to free hydroxyl and carboxyl groups of the core structure to form the peripheral structure.
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来源期刊
Nature Plants
Nature Plants PLANT SCIENCES-
CiteScore
25.30
自引率
2.20%
发文量
196
期刊介绍: Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.
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