大麦的生殖发育和热适应需要 TFIIS。

IF 5.3 2区 生物学 Q1 PLANT SCIENCES
Imtiaz Ahmad, András Kis, Radhika Verma, István Szádeczky-Kardoss, Henrik Mihály Szaker, Aladár Pettkó-Szandtner, Dániel Silhavy, Zoltán Havelda, Tibor Csorba
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引用次数: 0

摘要

关键信息:大麦的生殖能力和对热胁迫的高效适应需要 RNAPII 的延伸辅助因子 TFIIS 的活性。转录机制的调控及其在不同胁迫条件下的适应作用在双子叶模式植物拟南芥中得到了广泛的研究,但我们对单子叶植物的了解仍然有限。TFIIS 是一种与 RNA 聚合酶 II 相关的转录延伸辅助因子。以前的研究表明,TFIIS 确保了高效的转录延伸,而这是拟南芥在热胁迫下生存所必需的。然而,TFIIS 在单子叶植物中的功能尚未得到分析。在本研究中,我们生成并研究了独立的 tfIIs-crispr 突变大麦品系。我们发现大麦的生殖发育和热胁迫存活都需要 TFIIS。tfIIs 突变体对 HS 敏感的分子基础是热胁迫蛋白转录本的表达迟缓,这导致 HSP 合子的后期积累、蛋白毒性增强并最终导致致死。我们还表明,TFIIS 在响应热时受到转录调控,这支持了这些控制元件在植物热适应方面的保守适应功能。总之,我们的研究结果为更好地理解单子叶作物的转录机制调控向前迈进了一步。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TFIIS is required for reproductive development and thermal adaptation in barley.

Key message: Barley reproductive fitness and efficient heat stress adaptation requires the activity of TFIIS, the elongation cofactor of RNAPII. Regulation of transcriptional machinery and its adaptive role under different stress conditions are studied extensively in the dicot model plant Arabidopsis, but our knowledge on monocot species remains elusive. TFIIS is an RNA polymerase II-associated transcription elongation cofactor. Previously, it was shown that TFIIS ensures efficient transcription elongation that is necessary for heat stress survival in A. thaliana. However, the function of TFIIS has not been analysed in monocots. In the present work, we have generated and studied independent tfIIs-crispr-mutant barley lines. We show that TFIIS is needed for reproductive development and heat stress survival in barley. The molecular basis of HS-sensitivity of tfIIs mutants is the retarded expression of heat stress protein transcripts, which leads to late accumulation of HSP chaperones, enhanced proteotoxicity and ultimately to lethality. We also show that TFIIS is transcriptionally regulated in response to heat, supporting a conserved adaptive function of these control elements for plant thermal adaptation. In sum, our results are a step forward for the better understanding of transcriptional machinery regulation in monocot crops.

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来源期刊
Plant Cell Reports
Plant Cell Reports 生物-植物科学
CiteScore
10.80
自引率
1.60%
发文量
135
审稿时长
3.2 months
期刊介绍: Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.
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