评估β-葡聚糖对癌变皮肤细胞和从黑色素瘤诱发动物模型中分离的线粒体的浓度依赖性双重效应。

IF 1.6 4区 医学 Q3 OPHTHALMOLOGY
Yalda Arast, Hanife Esfandiari, Farzane Kamranfar, Zahra Mousavi, Mahdieh Ameri Shah Reza, Jalal Pourahmad
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引用次数: 0

摘要

简介黑色素瘤仍然是最致命的癌症之一,近几十年来发病率不断上升。如今,许多多糖及其生物活性化合物在现代生物技术中具有特别重要的地位。它们可以调节和增强免疫系统,降低血压和胆固醇,减少细菌和病毒感染。我们研究了从食用药用蘑菇 Lentinus edodes 的固态发酵(SSF)中获得的 β-葡聚糖对皮肤癌细胞的细胞毒性作用:通过差速离心从黑色素瘤细胞中分离出线粒体,并用不同浓度(30、45、60、90、120 和 240 µg/ml)的β-葡聚糖提取物处理。然后,对它们进行 MTT、ROS、MMP 下降、线粒体肿胀、细胞色素 c 释放和流式细胞术检测:MTT 试验结果表明,β-葡聚糖提取物的 IC50 值为 60 μg/ml,它能选择性地显著增加 ROS(P β-葡聚糖提取物能显著增加 ROS。然而,在更高浓度下没有观察到这种效应。与对照组相比,在 30、45 和 60 µg/ml 浓度下,癌症组线粒体和肿胀中的 MMP 下降和细胞色素 c 释放明显增加。在更高浓度下,未观察到此类影响。浓度为 60 µg/ml 的β-葡聚糖提取物可增加黑色素瘤细胞的凋亡,而对对照组非肿瘤细胞没有影响:根据上述结果,浓度为 30、45 和 60 µg/ml 的 β-葡聚糖提取物具有细胞毒性作用,而浓度较高时则没有这种作用。总体看来,β-葡聚糖在较高浓度下对癌细胞具有抗氧化和清除自由基的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluating the concentration dependent dual effects of β-Glucan on cancerous skin cells and mitochondria isolated from melanoma-induced animal model.

Introduction: Melanoma is still one of the deadliest cancers whose prevalence has increased in recent decades. Today, many polysaccharides and their bioactive compounds have been of special importance in modern biotechnology. They have various biological and therapeutic properties. they can regulate and strengthen the immune system, lower blood pressure and cholesterol, and reduce bacterial and viral infections. According to studies, these compounds also have antitumor properties. we investigated the cytotoxic effects of β-Glucan obtained from solid-state fermentation (SSF) of edible medicinal mushroom Lentinus edodes on cancerous skin cells.

Materials and methods: The mitochondria were isolated from melanoma cells via differential centrifugation and treated with various concentrations (30, 45, 60, 90, 120, and 240 µg/ml) of β-Glucan extract. Then, they were subjected to MTT, ROS, MMP decline, mitochondrial swelling, cytochrome c release, and flow cytometry assays.

Results: The results of the MTT assay showed that IC50 of β-Glucan extract was 60 μg/ml, and it induced a selectively significant (P < 0.05) concentration-dependent decrease in the SDH activity in cancerous skin mitochondria. At higher concentrations, no such effect was observed. The ROS results also showed that 30, 45, and 60 µg/ml concentrations of β-Glucan extract significantly increased ROS. However, no such effect was observed at higher concentrations. MMP decline and the release of cytochrome c in cancer groups mitochondria and swelling were significantly increased at 30, 45, and 60 µg/ml compared to the control group. At higher concentrations, no such effect was observed. β-Glucan extract at 60 µg/ml concentration increased apoptosis on melanoma cells, while it had no effect on control non-tumour cells.

Discussion and conclusion: Based on these results, β-Glucan extract at 30, 45, and 60 µg/ml showed a cytotoxic effect, while no such effect was observed at higher concentrations. Overall, it seems that β-Glucan has antioxidant and free radical scavenging effects on cancer cells at higher concentrations.

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来源期刊
CiteScore
3.30
自引率
6.20%
发文量
40
审稿时长
1 months
期刊介绍: Cutaneous and Ocular Toxicology is an international, peer-reviewed journal that covers all types of harm to cutaneous and ocular systems. Areas of particular interest include pharmaceutical and medical products; consumer, personal care, and household products; and issues in environmental and occupational exposures. In addition to original research papers, reviews and short communications are invited, as well as concise, relevant, and critical reviews of topics of contemporary significance.
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