首次从突尼斯蛋鸡养殖场的临床样本中检测禽支原体的分子调查。

IF 2.3 2区 农林科学 Q1 VETERINARY SCIENCES
Soumaya Sabri, Lamia Khannous, Séverine Ferré, Adel Souissi, Radhouane Gdoura, Anne V Gautier-Bouchardon
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引用次数: 0

摘要

背景:禽支原体是已知的病原体,会给禽群造成严重的经济损失。与培养法相比,PCR 是一种快速、灵敏且成本较低的诊断工具,可用于鉴定家禽养殖场中的支原体。本研究的目的是通过 PCR 确定突尼斯南部斯法克斯地区蛋鸡中是否存在支原体属、胆囊支原体(MG)、滑液囊支原体(MS)和拉氏支原体(MP):从 13 个蛋鸡养殖场共采集了 781 份气管拭子,采样时未发现临床症状。采用一种新的特异性 PCR 方法检测 MP。根据 PCR 结果计算出的鸡群流行率为:支原体 100%、MG 0%、MS 84.6%、MP 61.5%。在动物层面,支原体的总体流行率为 38.7%,MG 为 0%,MS 为 25%,MP 为 6.4%。蛋鸡养殖场禽支原体的总体流行率为 100%(动物水平的流行率为 38.7%),情况令人担忧:这些结果凸显了监测养殖场支原体菌株的出现和传播以减少支原体病造成的经济损失的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
First molecular investigation to detect avian Mycoplasma species in clinical samples from laying-hen farms in Tunisia.

Background: Avian mycoplasmas are known pathogens, which cause severe economic losses in poultry flocks. PCR is a rapid, sensitive, and less expensive diagnostic tool than culture for the identification of mycoplasmas in poultry farms. The objective of this study was to determine by PCR the presence of Mycoplasma spp., Mycoplasma gallisepticum (MG), Mycoplasma synoviae (MS), and Mycoplasma pullorum (MP) in laying hens located in the Sfax region, in the South of Tunisia.

Results: A total of 781 tracheal swabs were collected from 13 laying-hen farms without clinical signs at the date of sampling. MP was detected by a newly described specific PCR assay. The prevalence calculated from PCR results at the flock level was 100% for Mycoplasma spp., 0% for MG, 84.6% for MS and 61.5% for MP. The overall prevalence at the animal level was 38.7% for Mycoplasma spp., 0% for MG, 25% for MS and 6.4% for MP. The overall prevalence of 100% of avian mycoplasmas in laying-hen farms (38.7% prevalence at the animal level) shows an alarming situation.

Conclusions: These results underline the importance of monitoring the emergence and spread of Mycoplasma strains in farms in order to decrease economic losses due to mycoplasmoses.

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来源期刊
BMC Veterinary Research
BMC Veterinary Research VETERINARY SCIENCES-
CiteScore
4.80
自引率
3.80%
发文量
420
审稿时长
3-6 weeks
期刊介绍: BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.
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