自然感染的牛和水牛中块状皮肤病病毒的分子检测:揭示蜱媒介在疾病传播中的作用。

IF 1.8 3区 农林科学 Q2 VETERINARY SCIENCES
Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-10-08 DOI:10.1007/s11259-024-10541-7
Gamil S G Zeedan, Abeer M Abdalhamed, Ahmad M Allam, Sobhy Abdel-Shafy
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引用次数: 0

摘要

结节性皮肤病(LSD)是一种影响埃及牛和水牛的病毒性疾病,给畜牧业造成了巨大的经济损失。本研究旨在通过多重 PCR(mPCR)和实时定量 PCR(rt-qPCR)分析法进行分离和分子鉴定,调查最近在牛和水牛中爆发的 LSDV,并评估硬蜱 Rhipicephalus annulatus 在其传播中的潜在作用。共收集了 50 份皮肤活检样本(牛样本 30 份,水牛样本 20 份)、110 份鼻拭子样本(牛样本 76 份,水牛样本 44 份)和 129 份血液样本(牛样本 84 份,水牛样本 45 份)。此外,2021 年 11 月至 2022 年 6 月期间,在埃及不同省份从不同品种和年龄的牛和水牛身上采集了 145 只不同阶段的硬蜱。我们使用了基于 SYBR Green 和靶标(P32、VP32、G 蛋白和病毒融合蛋白)的多重 PCR 和实时定量 PCR (rt-qPCR) 检测方法。使用 mPCR 和 RT-qPCR 方法,我们在 30 份牛皮肤活检样本中有 17 份(56.6%)、7 份水牛皮肤痂皮样本中有 1 份(14.3%)以及 45 份水牛血液样本中有 5 份(11.11%)鉴定出阳性结果。我们成功地从硬蜱和受蜱虫侵扰的牛身上分离出了 LSDV,并在特异性无病原体胚胎鸡卵(SPF-ECE)的绒毛膜(CAM)上发现了 LSD 的特征性症状。通过多重 PCR 和 RT-qPCR 对分离物进行了确认。rt-qPCR 的循环阈值(Ct)和相关带状曲线值从 10.2 到 36.5 不等,显示了不同样品中 LSDV-DNA 的含量。研究结果表明,LSDV 在埃及的牛和水牛中广泛流行,并提供了有力的证据,证明硬蜱 R. annulatus 在易感动物中传播 LSDV 的过程中发挥了作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular detection of lumpy skin disease virus in naturally infected cattle and buffaloes: unveiling the role of tick vectors in disease spread.

Lumpy skin disease (LSD) is a viral disease that affects cattle and buffaloes in Egypt, causing considerable economic losses in the animal sector. This study aimed to investigate the recent outbreak of LSDV in cattle and buffaloes and evaluate the potential role of the hard tick Rhipicephalus annulatus in their transmission through isolation and molecular characterization by multiplex PCR (mPCR) and real-time quantitative PCR (rt-qPCR) assays. A total of 50 skin biopsies (cattle n = 30, buffaloes n = 20), 110 nasal swabs (cattle n = 76, buffaloes n = 44), and 129 blood samples (cattle n = 84, buffaloes n = 45) were collected. In addition, 145 hard ticks of different stages were collected from cattle and buffaloes of different breeds and ages in different governorates in Egypt from November 2021 to June 2022. Multiplex PCR and real-time quantitative PCR (rt-qPCR) assays based on SYBR Green and targets (P32, VP32, G protein, and viral fusion protein) were used. We identified positive results in 17 out of 30 cattle skin biopsies (56.6%), 1 out of 7 buffalo skin scabs (14.3%), and 5 out of 45 buffalo blood samples (11.11%) using mPCR and RT-qPCR methods. We successfully isolated LSDV from hard ticks and cattle infested with ticks and exhibited characteristic signs of LSD on the chorioallantois membrane (CAM) of specific pathogen-free embryonated chicken eggs (SPF-ECE). The isolates were confirmed by multiplex PCR and RT-qPCR. The cyclic threshold (Ct) with correlation-slandered curve values of rt-qPCR ranging from 10.2 to 36.5 showed the amount of LSDV-DNA in different samples. The study's findings demonstrated the widespread circulation of LSDV in both cattle and buffaloes in Egypt and provided strong evidence that hard ticks R. annulatus play a role in the transmission of LSDV in susceptible animals.

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来源期刊
Veterinary Research Communications
Veterinary Research Communications 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
173
审稿时长
3 months
期刊介绍: Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.
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