花生(Arachis hypogaea L.)荚果和果仁性状数量性状位点的共定位以及染色体 Arahy05 上果仁重量分子标记的开发。

IF 4.4 1区 农林科学 Q1 AGRONOMY
Yuanjin Fang, Hua Liu, Ziqi Sun, Li Qin, Zheng Zheng, Feiyan Qi, Jihua Wu, Wenzhao Dong, Bingyan Huang, Xinyou Zhang
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引用次数: 0

摘要

关键信息:荚果和果仁性状的稳定 QTL 共同定位在染色体 Arahy05 上,Arahy05 上 106,411,957 处的 INDEL 标记经开发和验证可用于果仁重量的标记辅助选择。在花生育种计划中,百荚重(HPW)和百仁重(HKW)等荚果和果仁性状以及荚果和果仁大小是决定产量的关键因素。本研究试图找出与花生这些豆荚和果仁性状相关的数量性状位点(QTL)。为此,研究人员从以高产著称的栽培品种 "裕华15 "和种质登录W1202杂交得到了一个重组近交系(RIL)群体。研究发现了与豆荚和果仁重量显著相关的稳定的主要 QTL,这些 QTL 始终共定位在染色体 Arahy05 和 Arahy08 上。此外,在 Arahy05 上的 QTL 区间还发现了一个 INDEL 标记,并对其进行了表征。通过对 395 份种质材料进行广泛的重测序分析,在上述 INDEL 标记侧翼的 500-kb 窗口内发现了两个主要单倍型。这些单倍型与我们不同种质组中的 HKW 有显著的相关性。值得注意的是,170 份含有与 HKW 增加相关的单倍型的种质主要代表植物品种,特别是 Arachis hypogaea var.另一方面,与另一种单倍型相关的 137 个登录品系则主要属于 A. hypogaea 亚种 fastigiata 中的植物品种。位于 Arahy05 上的 INDEL 标记与豆荚和果仁的性状有密切联系,是育种计划中对豆荚和果仁重量进行标记辅助选择(MAS)的有效方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Co-localization of quantitative trait loci for pod and kernel traits and development of molecular marker for kernel weight on chromosome Arahy05 in peanut (Arachis hypogaea L.).

Key message: Stable QTL for pod and kernel traits were co-localized on chromosome Arahy05, and an INDEL marker at 106,411,957 on Arahy05 was developed and validated to be useful for marker-assisted selection of kernel weight. Pod and kernel traits, such as hundred pod weight (HPW), and hundred kernel weight (HKW), along with pod and kernel sizes, are pivotal determinants of yield in peanut breeding programs. This study sought to identify quantitative trait loci (QTL) that are associated with these pod and kernel traits in peanuts. To achieve this, a recombinant inbred line (RIL) population, was derived from a cross between Yuhua15, a cultivar known for its high yield, and a germplasm accession W1202. The investigation uncovered stable and major QTL that are significantly associated with both pod and kernel weight and were consistently co-localized on chromosomes Arahy05 and Arahy08. Furthermore, an INDEL marker was identified and characterized in the QTL interval on Arahy05. An extensive re-sequencing analysis comprising 395 germplasm accessions led to the discovery of two principal haplotypes within a 500-kb window flanking the aforementioned INDEL marker. The haplotypes exhibited a significant correlation with the HKW in our diverse panel of germplasm accessions. Notably, the 170 accessions harboring the haplotype associated with an increased HKW primarily represented botanical varieties, specifically Arachis hypogaea var. hypogaea and A. hypogaea var. hirsuta. On the other hand, the 137 accessions associated with the alternative haplotype, which corresponded to a reduced HKW, were predominately identified as belonging to botanical varieties within A. hypogaea subsp. fastigiata. The INDEL marker located on Arahy05, which demonstrates close linkage to the pod and kernel traits, would be an efficient approach for marker-assisted selection (MAS) of pod and kernel weight in breeding programs.

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来源期刊
CiteScore
9.60
自引率
7.40%
发文量
241
审稿时长
2.3 months
期刊介绍: Theoretical and Applied Genetics publishes original research and review articles in all key areas of modern plant genetics, plant genomics and plant biotechnology. All work needs to have a clear genetic component and significant impact on plant breeding. Theoretical considerations are only accepted in combination with new experimental data and/or if they indicate a relevant application in plant genetics or breeding. Emphasizing the practical, the journal focuses on research into leading crop plants and articles presenting innovative approaches.
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