{"title":"从栉水母肠道中提取的益生菌株植物乳杆菌 GCLP4 可调节鲮鱼幼鱼的生长、消化酶和免疫生化指标","authors":"Rubina Yasmin, Pabitra Kumar Saharia, Inam Akhtar Hussain, Avinash Talukdar, Sangipran Baishya, Kaustubh Bhagawati, Dipak kr Sarma, Arnab Narayan Patowary, Kamaleswar Kalita, Utpal Kumar Das, Aprajita Singh","doi":"10.1111/jpn.14054","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>The present study was carried out to isolate, identify, and characterize bacterial probiotic strain from the gut of <i>Ctenopharyngodon idella</i> (Grass carp) and then to study its effect on growth, digestive enzymes and immunity of <i>Labeo rohita</i> fingerlings. A total of 6 gram-positive bacteria belonging to the genus <i>Lactobacillus</i> spp. (3), <i>Bacillus</i> spp. (2) and <i>Staphylococcus</i> spp. (1), were identified biochemically. Based on the biochemical results, the isolate GCLP4 was selected for molecular confirmation and BLAST analysis showed maximum homology with <i>Lactobacillus plantarum</i> (100% ident). Fish were fed for 60 days with diet containing 0 (T0), 10<sup>5</sup> (T1), 10<sup>7</sup> (T2), 10<sup>9</sup> (T3) cfu/g diet of <i>L. plantarum</i> GCLP4 and 10<sup>5</sup> (T4) of commercial probiotics. Results shows that supplementation of GCLP4 had significantly (<i>p</i> < 0.05) improve weight gain (%), specific growth rate (SGR) and feed conversion ratio (FCR) of <i>L. rohita</i> with better values in T3 group. The immunological parameters (white blood cell, red blood cell, haemoglobin, total protein, albumin and globulin were significantly higher (<i>p</i> < 0.05) with 10<sup>7</sup> and 10<sup>9</sup> <i>Lactobacillus</i> GCLP4 cfu/g diet. The digestive enzyme activities (protease, amylase and lipase) were significantly (<i>p</i> < 0.05) higher, particularly, with 10<sup>9</sup> <i>Lactobacillus</i> GCLP4 cfu/g of diet. All the groups supplemented with GCLP4 including the commercial probiotics have lower (<i>p</i> < 0.05) activities of serum transaminase enzymes along with lower (<i>p</i> < 0.005) level of glucose as compared to the control group. The results of the study collectively suggest that dietary <i>L. plantarum</i> GCLP4 at 10<sup>9</sup> cfu/g is an effective probiotic obtained from grass carp having potency to promote growth, digestive enzymes and immune-biochemical indices of <i>L. rohita</i> fingerlings in present culture condition.</p>\n </div>","PeriodicalId":14942,"journal":{"name":"Journal of Animal Physiology and Animal Nutrition","volume":"109 2","pages":"268-279"},"PeriodicalIF":2.4000,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Probiotic Strain Lactobacillus plantarum GCLP4 Derived From the Gut of Ctenopharyngodon idella Modulates Growth, Digestive Enzymes and Immune-Biochemical Parameters of Labeo rohita Fingerlings\",\"authors\":\"Rubina Yasmin, Pabitra Kumar Saharia, Inam Akhtar Hussain, Avinash Talukdar, Sangipran Baishya, Kaustubh Bhagawati, Dipak kr Sarma, Arnab Narayan Patowary, Kamaleswar Kalita, Utpal Kumar Das, Aprajita Singh\",\"doi\":\"10.1111/jpn.14054\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n <p>The present study was carried out to isolate, identify, and characterize bacterial probiotic strain from the gut of <i>Ctenopharyngodon idella</i> (Grass carp) and then to study its effect on growth, digestive enzymes and immunity of <i>Labeo rohita</i> fingerlings. A total of 6 gram-positive bacteria belonging to the genus <i>Lactobacillus</i> spp. (3), <i>Bacillus</i> spp. (2) and <i>Staphylococcus</i> spp. (1), were identified biochemically. Based on the biochemical results, the isolate GCLP4 was selected for molecular confirmation and BLAST analysis showed maximum homology with <i>Lactobacillus plantarum</i> (100% ident). Fish were fed for 60 days with diet containing 0 (T0), 10<sup>5</sup> (T1), 10<sup>7</sup> (T2), 10<sup>9</sup> (T3) cfu/g diet of <i>L. plantarum</i> GCLP4 and 10<sup>5</sup> (T4) of commercial probiotics. Results shows that supplementation of GCLP4 had significantly (<i>p</i> < 0.05) improve weight gain (%), specific growth rate (SGR) and feed conversion ratio (FCR) of <i>L. rohita</i> with better values in T3 group. The immunological parameters (white blood cell, red blood cell, haemoglobin, total protein, albumin and globulin were significantly higher (<i>p</i> < 0.05) with 10<sup>7</sup> and 10<sup>9</sup> <i>Lactobacillus</i> GCLP4 cfu/g diet. The digestive enzyme activities (protease, amylase and lipase) were significantly (<i>p</i> < 0.05) higher, particularly, with 10<sup>9</sup> <i>Lactobacillus</i> GCLP4 cfu/g of diet. All the groups supplemented with GCLP4 including the commercial probiotics have lower (<i>p</i> < 0.05) activities of serum transaminase enzymes along with lower (<i>p</i> < 0.005) level of glucose as compared to the control group. The results of the study collectively suggest that dietary <i>L. plantarum</i> GCLP4 at 10<sup>9</sup> cfu/g is an effective probiotic obtained from grass carp having potency to promote growth, digestive enzymes and immune-biochemical indices of <i>L. rohita</i> fingerlings in present culture condition.</p>\\n </div>\",\"PeriodicalId\":14942,\"journal\":{\"name\":\"Journal of Animal Physiology and Animal Nutrition\",\"volume\":\"109 2\",\"pages\":\"268-279\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2024-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Animal Physiology and Animal Nutrition\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/jpn.14054\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Animal Physiology and Animal Nutrition","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/jpn.14054","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Probiotic Strain Lactobacillus plantarum GCLP4 Derived From the Gut of Ctenopharyngodon idella Modulates Growth, Digestive Enzymes and Immune-Biochemical Parameters of Labeo rohita Fingerlings
The present study was carried out to isolate, identify, and characterize bacterial probiotic strain from the gut of Ctenopharyngodon idella (Grass carp) and then to study its effect on growth, digestive enzymes and immunity of Labeo rohita fingerlings. A total of 6 gram-positive bacteria belonging to the genus Lactobacillus spp. (3), Bacillus spp. (2) and Staphylococcus spp. (1), were identified biochemically. Based on the biochemical results, the isolate GCLP4 was selected for molecular confirmation and BLAST analysis showed maximum homology with Lactobacillus plantarum (100% ident). Fish were fed for 60 days with diet containing 0 (T0), 105 (T1), 107 (T2), 109 (T3) cfu/g diet of L. plantarum GCLP4 and 105 (T4) of commercial probiotics. Results shows that supplementation of GCLP4 had significantly (p < 0.05) improve weight gain (%), specific growth rate (SGR) and feed conversion ratio (FCR) of L. rohita with better values in T3 group. The immunological parameters (white blood cell, red blood cell, haemoglobin, total protein, albumin and globulin were significantly higher (p < 0.05) with 107 and 109Lactobacillus GCLP4 cfu/g diet. The digestive enzyme activities (protease, amylase and lipase) were significantly (p < 0.05) higher, particularly, with 109Lactobacillus GCLP4 cfu/g of diet. All the groups supplemented with GCLP4 including the commercial probiotics have lower (p < 0.05) activities of serum transaminase enzymes along with lower (p < 0.005) level of glucose as compared to the control group. The results of the study collectively suggest that dietary L. plantarum GCLP4 at 109 cfu/g is an effective probiotic obtained from grass carp having potency to promote growth, digestive enzymes and immune-biochemical indices of L. rohita fingerlings in present culture condition.
期刊介绍:
As an international forum for hypothesis-driven scientific research, the Journal of Animal Physiology and Animal Nutrition publishes original papers in the fields of animal physiology, biochemistry and physiology of nutrition, animal nutrition, feed technology and preservation (only when related to animal nutrition). Well-conducted scientific work that meets the technical and ethical standards is considered only on the basis of scientific rigor.
Research on farm and companion animals is preferred. Comparative work on exotic species is welcome too. Pharmacological or toxicological experiments with a direct reference to nutrition are also considered. Manuscripts on fish and other aquatic non-mammals with topics on growth or nutrition will not be accepted. Manuscripts may be rejected on the grounds that the subject is too specialized or that the contribution they make to animal physiology and nutrition is insufficient.
In addition, reviews on topics of current interest within the scope of the journal are welcome. Authors are advised to send an outline to the Editorial Office for approval prior to submission.