从栉水母肠道中提取的益生菌株植物乳杆菌 GCLP4 可调节鲮鱼幼鱼的生长、消化酶和免疫生化指标

IF 2.4 3区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Rubina Yasmin, Pabitra Kumar Saharia, Inam Akhtar Hussain, Avinash Talukdar, Sangipran Baishya, Kaustubh Bhagawati, Dipak kr Sarma, Arnab Narayan Patowary, Kamaleswar Kalita, Utpal Kumar Das, Aprajita Singh
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引用次数: 0

摘要

本研究旨在从草鱼(Ctenopharyngodon idella)肠道中分离、鉴定和表征益生菌菌株,然后研究其对鲮鱼幼鱼的生长、消化酶和免疫力的影响。经生化鉴定,共有 6 种革兰氏阳性细菌,分别属于乳酸杆菌属(3 种)、芽孢杆菌属(2 种)和葡萄球菌属(1 种)。根据生化结果,选择分离物 GCLP4 进行分子确认,BLAST 分析显示其与植物乳杆菌的同源性最高(100% 相同)。用含 0(T0)、105(T1)、107(T2)、109(T3)cfu/g 植物乳杆菌 GCLP4 和 105(T4)商业益生菌的日粮喂鱼 60 天。结果表明,添加 GCLP4 和 109 Lactobacillus GCLP4 cfu/g 日粮有明显的效果(p 7 和 109 Lactobacillus GCLP4 cfu/g 日粮)。消化酶活性(蛋白酶、淀粉酶和脂肪酶)明显高于(p 9 乳酸杆菌 GCLP4 cfu/g 日粮)。GCLP4 是一种从草鱼中提取的有效益生菌,在目前的养殖条件下具有促进鲤鱼幼体生长、消化酶和免疫生化指标的功效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Probiotic Strain Lactobacillus plantarum GCLP4 Derived From the Gut of Ctenopharyngodon idella Modulates Growth, Digestive Enzymes and Immune-Biochemical Parameters of Labeo rohita Fingerlings

The present study was carried out to isolate, identify, and characterize bacterial probiotic strain from the gut of Ctenopharyngodon idella (Grass carp) and then to study its effect on growth, digestive enzymes and immunity of Labeo rohita fingerlings. A total of 6 gram-positive bacteria belonging to the genus Lactobacillus spp. (3), Bacillus spp. (2) and Staphylococcus spp. (1), were identified biochemically. Based on the biochemical results, the isolate GCLP4 was selected for molecular confirmation and BLAST analysis showed maximum homology with Lactobacillus plantarum (100% ident). Fish were fed for 60 days with diet containing 0 (T0), 105 (T1), 107 (T2), 109 (T3) cfu/g diet of L. plantarum GCLP4 and 105 (T4) of commercial probiotics. Results shows that supplementation of GCLP4 had significantly (p < 0.05) improve weight gain (%), specific growth rate (SGR) and feed conversion ratio (FCR) of L. rohita with better values in T3 group. The immunological parameters (white blood cell, red blood cell, haemoglobin, total protein, albumin and globulin were significantly higher (p < 0.05) with 107 and 109 Lactobacillus GCLP4 cfu/g diet. The digestive enzyme activities (protease, amylase and lipase) were significantly (p < 0.05) higher, particularly, with 109 Lactobacillus GCLP4 cfu/g of diet. All the groups supplemented with GCLP4 including the commercial probiotics have lower (p < 0.05) activities of serum transaminase enzymes along with lower (p < 0.005) level of glucose as compared to the control group. The results of the study collectively suggest that dietary L. plantarum GCLP4 at 109 cfu/g is an effective probiotic obtained from grass carp having potency to promote growth, digestive enzymes and immune-biochemical indices of L. rohita fingerlings in present culture condition.

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来源期刊
Journal of Animal Physiology and Animal Nutrition
Journal of Animal Physiology and Animal Nutrition 农林科学-奶制品与动物科学
CiteScore
6.30
自引率
0.00%
发文量
124
审稿时长
2 months
期刊介绍: As an international forum for hypothesis-driven scientific research, the Journal of Animal Physiology and Animal Nutrition publishes original papers in the fields of animal physiology, biochemistry and physiology of nutrition, animal nutrition, feed technology and preservation (only when related to animal nutrition). Well-conducted scientific work that meets the technical and ethical standards is considered only on the basis of scientific rigor. Research on farm and companion animals is preferred. Comparative work on exotic species is welcome too. Pharmacological or toxicological experiments with a direct reference to nutrition are also considered. Manuscripts on fish and other aquatic non-mammals with topics on growth or nutrition will not be accepted. Manuscripts may be rejected on the grounds that the subject is too specialized or that the contribution they make to animal physiology and nutrition is insufficient. In addition, reviews on topics of current interest within the scope of the journal are welcome. Authors are advised to send an outline to the Editorial Office for approval prior to submission.
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