C-反应蛋白不是溃疡性结肠炎的驱动因素

IF 2 4区 医学 Q3 GASTROENTEROLOGY & HEPATOLOGY
Gastroenterology Research and Practice Pub Date : 2024-10-01 eCollection Date: 2024-01-01 DOI:10.1155/2024/1386147
Zhong-Bo Ge, Xin-Yun Zhang, Chun-Miao Zhang, Tao-Tao Xu, Si-Yi Li, Meng-Xiao Wei, Xin-Yuan Ding, Cai-Juan Bai, Han Wang, Hai-Hong Zhou, Ming-Yu Wang
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引用次数: 0

摘要

目的:C反应蛋白(CRP)是各种炎症性疾病的非特异性标志物,尤其是在评估溃疡性结肠炎患者的药物治疗效果时。现有证据不足以支持 CRP 直接影响溃疡性结肠炎的病情发展。研究方法我们的研究采用了严格的小鼠模型。通过让 CRP 缺乏的小鼠接受葡聚糖硫酸钠(DSS)治疗,建立了溃疡性结肠炎小鼠模型。我们对小鼠的表型(包括体重、结肠长度和脾脏重量等参数)进行了细致的评估。此外,还评估了各种生理和生化指标,包括结肠组织病理学、炎症因子表达水平和肠粘液层染色。结果显示与具有野生型 CRP 的小鼠相比,缺乏 CRP 对溃疡性结肠炎小鼠模型的表型、生理特征和生化指标没有明显影响。此外,通过抗生素治疗消除肠道菌群干扰后发现,缺乏 CRP 的小鼠在溃疡性结肠炎模型中没有表现出任何明显的变化。同时,与野生型小鼠相比,缺乏 CRP 小鼠的存活率在统计学上没有显著差异。结论我们的研究结果表明,CRP 可能不会直接介导溃疡性结肠炎。相反,它更可能是与炎症因子升高同时存在的旁观者。由于使用小鼠模型存在很大的局限性,因此有必要进行进一步研究,以确定 CRP 在人类中的确切作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
C-Reactive Protein Is Not the Driver Factor in Ulcerative Colitis.

Purpose: C-reactive protein (CRP) functions as a nonspecific marker in various inflammatory disorders, particularly in evaluating the efficacy of pharmacological treatments in patients with ulcerative colitis. The existing body of evidence does not offer adequate support for the direct implication of CRP in modulating the advancement of ulcerative colitis. Methods: Our study employed a rigorous mouse model. An ulcerative colitis mouse model was established by subjecting CRP-deficient mice to dextran sulfate sodium (DSS) treatment. The phenotype of the mice, which encompassed parameters such as body weight, colon length, and spleen weight, was meticulously evaluated. Additionally, various physiological and biochemical indicators were assessed, including colon histopathology, expression levels of inflammatory factors, and staining of the intestinal mucus layer. Results: The absence of CRP did not significantly affect the phenotype, physiological characteristics, and biochemical indices in a mouse model of ulcerative colitis compared to mice with wild-type CRP. Additionally, eliminating intestinal bacteria flora interference through antibiotic treatment revealed that mice lacking CRP did not demonstrate any notable variations in the ulcerative colitis model. Meanwhile, the survival rate of mice lacking CRP did not exhibit a statistically significant difference compared to wild-type mice. Conclusion: The results of our study suggest that CRP may not directly mediate ulcerative colitis. Instead, it is more likely to be a bystander that is present alongside with elevated inflammatory factors. Further investigation is warranted to determine the precise role of CRP in humans, given the significant limitations associated with the use of mouse models.

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来源期刊
Gastroenterology Research and Practice
Gastroenterology Research and Practice GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
4.40
自引率
0.00%
发文量
91
审稿时长
1 months
期刊介绍: Gastroenterology Research and Practice is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on all areas of gastroenterology, hepatology, pancreas and biliary, and related cancers. The journal welcomes submissions on the physiology, pathophysiology, etiology, diagnosis and therapy of gastrointestinal diseases. The aim of the journal is to provide cutting edge research related to the field of gastroenterology, as well as digestive diseases and disorders. Topics of interest include: Management of pancreatic diseases Third space endoscopy Endoscopic resection Therapeutic endoscopy Therapeutic endosonography.
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