Juan F Villacís, Andrea López-Rosero, Juan José Bustillos, Matías Cadena, César A Yumiseva, Mario J Grijalva, Anita G Villacís
{"title":"厄瓜多尔中央海岸和安第斯山脉南部恰加斯病病媒 Rhodnius ecuadoriensis 肠道中的细菌微生物群。","authors":"Juan F Villacís, Andrea López-Rosero, Juan José Bustillos, Matías Cadena, César A Yumiseva, Mario J Grijalva, Anita G Villacís","doi":"10.3389/fmicb.2024.1464720","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Chagas disease is a neglected tropical disease caused by the parasite <i>Trypanosoma cruzi</i> that is transmitted mainly by the feces of infected Triatomines. In Ecuador the main vector is <i>Rhodnius ecuadoriensis</i> which is distributed in several provinces of the country. More than 40% of these insects in the wild have <i>T. cruzi</i> as part of their intestinal microbiota. For this reason, the objective of this research was to characterize the intestinal bacterial microbiota of <i>R. ecuadoriensis</i>.</p><p><strong>Methods: </strong>The methodology used was based on the DNA extraction of the intestinal contents from the wild collected insects (adults and nymphs V), as well as the insects maintained at the insectary of the CISeAL. Finally, the samples were analyzed by metagenomics extensions based on the different selected criteria.</p><p><strong>Results: </strong>The intestinal microbiota of <i>R. ecuadoriensis</i> presented a marked divergence between laboratory-raised and wild collected insects. This difference was observed in all stages and was similar between insects from Loja and Manabí. A large loss of microbial symbionts was observed in laboratory-raised insects.</p><p><strong>Discussion: </strong>This study is a crucial first step in investigating microbiota interactions and advancing new methodologies.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11456480/pdf/","citationCount":"0","resultStr":"{\"title\":\"Bacterial microbiota from the gut of <i>Rhodnius ecuadoriensis</i>, a vector of Chagas disease in Ecuador's Central Coast and Southern Andes.\",\"authors\":\"Juan F Villacís, Andrea López-Rosero, Juan José Bustillos, Matías Cadena, César A Yumiseva, Mario J Grijalva, Anita G Villacís\",\"doi\":\"10.3389/fmicb.2024.1464720\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Chagas disease is a neglected tropical disease caused by the parasite <i>Trypanosoma cruzi</i> that is transmitted mainly by the feces of infected Triatomines. In Ecuador the main vector is <i>Rhodnius ecuadoriensis</i> which is distributed in several provinces of the country. More than 40% of these insects in the wild have <i>T. cruzi</i> as part of their intestinal microbiota. For this reason, the objective of this research was to characterize the intestinal bacterial microbiota of <i>R. ecuadoriensis</i>.</p><p><strong>Methods: </strong>The methodology used was based on the DNA extraction of the intestinal contents from the wild collected insects (adults and nymphs V), as well as the insects maintained at the insectary of the CISeAL. Finally, the samples were analyzed by metagenomics extensions based on the different selected criteria.</p><p><strong>Results: </strong>The intestinal microbiota of <i>R. ecuadoriensis</i> presented a marked divergence between laboratory-raised and wild collected insects. This difference was observed in all stages and was similar between insects from Loja and Manabí. A large loss of microbial symbionts was observed in laboratory-raised insects.</p><p><strong>Discussion: </strong>This study is a crucial first step in investigating microbiota interactions and advancing new methodologies.</p>\",\"PeriodicalId\":12466,\"journal\":{\"name\":\"Frontiers in Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11456480/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3389/fmicb.2024.1464720\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmicb.2024.1464720","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Bacterial microbiota from the gut of Rhodnius ecuadoriensis, a vector of Chagas disease in Ecuador's Central Coast and Southern Andes.
Introduction: Chagas disease is a neglected tropical disease caused by the parasite Trypanosoma cruzi that is transmitted mainly by the feces of infected Triatomines. In Ecuador the main vector is Rhodnius ecuadoriensis which is distributed in several provinces of the country. More than 40% of these insects in the wild have T. cruzi as part of their intestinal microbiota. For this reason, the objective of this research was to characterize the intestinal bacterial microbiota of R. ecuadoriensis.
Methods: The methodology used was based on the DNA extraction of the intestinal contents from the wild collected insects (adults and nymphs V), as well as the insects maintained at the insectary of the CISeAL. Finally, the samples were analyzed by metagenomics extensions based on the different selected criteria.
Results: The intestinal microbiota of R. ecuadoriensis presented a marked divergence between laboratory-raised and wild collected insects. This difference was observed in all stages and was similar between insects from Loja and Manabí. A large loss of microbial symbionts was observed in laboratory-raised insects.
Discussion: This study is a crucial first step in investigating microbiota interactions and advancing new methodologies.
期刊介绍:
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.