{"title":"用 \"爱情匹配 \"评分法比较枯草芽孢杆菌、荧光假单胞菌和巴西绿氮菌对植物生长促进根瘤菌的根渗出物吸引力和喂养。","authors":"Eulalie Fourneau, Mélissa Pannier, Wassila Riah, Emmanuelle Personeni, Annette Morvan-Bertrand, Josselin Bodilis, Barbara Pawlak","doi":"10.3389/fmicb.2024.1473099","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>The rhizosphere is the zone of soil surrounding plant roots that is directly influenced by root exudates released by the plant, which select soil microorganisms. The resulting rhizosphere microbiota plays a key role in plant health and development by enhancing its nutrition or immune response and protecting it from biotic or abiotic stresses. In particular, plant growth-promoting rhizobacteria (PGPR) are beneficial members of this microbiota that represent a great hope for agroecology, since they could be used as bioinoculants for sustainable crop production. Therefore, it is necessary to decipher the molecular dialog between roots and PGPR in order to promote the establishment of bioinoculants in the rhizosphere, which is required for their beneficial functions.</p><p><strong>Methods: </strong>Here, the ability of root exudates from rapeseed (<i>Brassica napus</i>), pea (<i>Pisum sativum</i>), and ryegrass (<i>Lolium perenne</i>) to attract and feed three PGPR (<i>Bacillus subtilis</i>, <i>Pseudomonas fluorescens</i>, and <i>Azospirillum brasilense</i>) was measured and compared, as these responses are directly involved in the establishment of the rhizosphere microbiota.</p><p><strong>Results: </strong>Our results showed that root exudates differentially attracted and fed the three PGPR. For all beneficial bacteria, rapeseed exudates were the most attractive and induced the fastest growth, while pea exudates allowed the highest biomass production. The performance of ryegrass exudates was generally lower, and variable responses were observed between bacteria. In addition, <i>P. fluorescens</i> and <i>A. brasilense</i> appeared to respond more efficiently to root exudates than <i>B. subtilis</i>. Finally, we proposed to evaluate the compatibility of each plant-PGPR couple by assigning them a \"love match\" score, which reflects the ability of root exudates to enhance bacterial rhizocompetence.</p><p><strong>Discussion: </strong>Taken together, our results provide new insights into the specific selection of PGPR by the plant through their root exudates and may help to select the most effective exudates to promote bioinoculant establishment in the rhizosphere.</p>","PeriodicalId":12466,"journal":{"name":"Frontiers in Microbiology","volume":null,"pages":null},"PeriodicalIF":4.0000,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11456545/pdf/","citationCount":"0","resultStr":"{\"title\":\"A \\\"love match\\\" score to compare root exudate attraction and feeding of the plant growth-promoting rhizobacteria <i>Bacillus subtilis</i>, <i>Pseudomonas fluorescens</i>, and <i>Azospirillum brasilense</i>.\",\"authors\":\"Eulalie Fourneau, Mélissa Pannier, Wassila Riah, Emmanuelle Personeni, Annette Morvan-Bertrand, Josselin Bodilis, Barbara Pawlak\",\"doi\":\"10.3389/fmicb.2024.1473099\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>The rhizosphere is the zone of soil surrounding plant roots that is directly influenced by root exudates released by the plant, which select soil microorganisms. The resulting rhizosphere microbiota plays a key role in plant health and development by enhancing its nutrition or immune response and protecting it from biotic or abiotic stresses. In particular, plant growth-promoting rhizobacteria (PGPR) are beneficial members of this microbiota that represent a great hope for agroecology, since they could be used as bioinoculants for sustainable crop production. Therefore, it is necessary to decipher the molecular dialog between roots and PGPR in order to promote the establishment of bioinoculants in the rhizosphere, which is required for their beneficial functions.</p><p><strong>Methods: </strong>Here, the ability of root exudates from rapeseed (<i>Brassica napus</i>), pea (<i>Pisum sativum</i>), and ryegrass (<i>Lolium perenne</i>) to attract and feed three PGPR (<i>Bacillus subtilis</i>, <i>Pseudomonas fluorescens</i>, and <i>Azospirillum brasilense</i>) was measured and compared, as these responses are directly involved in the establishment of the rhizosphere microbiota.</p><p><strong>Results: </strong>Our results showed that root exudates differentially attracted and fed the three PGPR. For all beneficial bacteria, rapeseed exudates were the most attractive and induced the fastest growth, while pea exudates allowed the highest biomass production. The performance of ryegrass exudates was generally lower, and variable responses were observed between bacteria. In addition, <i>P. fluorescens</i> and <i>A. brasilense</i> appeared to respond more efficiently to root exudates than <i>B. subtilis</i>. Finally, we proposed to evaluate the compatibility of each plant-PGPR couple by assigning them a \\\"love match\\\" score, which reflects the ability of root exudates to enhance bacterial rhizocompetence.</p><p><strong>Discussion: </strong>Taken together, our results provide new insights into the specific selection of PGPR by the plant through their root exudates and may help to select the most effective exudates to promote bioinoculant establishment in the rhizosphere.</p>\",\"PeriodicalId\":12466,\"journal\":{\"name\":\"Frontiers in Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.0000,\"publicationDate\":\"2024-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11456545/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3389/fmicb.2024.1473099\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3389/fmicb.2024.1473099","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
A "love match" score to compare root exudate attraction and feeding of the plant growth-promoting rhizobacteria Bacillus subtilis, Pseudomonas fluorescens, and Azospirillum brasilense.
Introduction: The rhizosphere is the zone of soil surrounding plant roots that is directly influenced by root exudates released by the plant, which select soil microorganisms. The resulting rhizosphere microbiota plays a key role in plant health and development by enhancing its nutrition or immune response and protecting it from biotic or abiotic stresses. In particular, plant growth-promoting rhizobacteria (PGPR) are beneficial members of this microbiota that represent a great hope for agroecology, since they could be used as bioinoculants for sustainable crop production. Therefore, it is necessary to decipher the molecular dialog between roots and PGPR in order to promote the establishment of bioinoculants in the rhizosphere, which is required for their beneficial functions.
Methods: Here, the ability of root exudates from rapeseed (Brassica napus), pea (Pisum sativum), and ryegrass (Lolium perenne) to attract and feed three PGPR (Bacillus subtilis, Pseudomonas fluorescens, and Azospirillum brasilense) was measured and compared, as these responses are directly involved in the establishment of the rhizosphere microbiota.
Results: Our results showed that root exudates differentially attracted and fed the three PGPR. For all beneficial bacteria, rapeseed exudates were the most attractive and induced the fastest growth, while pea exudates allowed the highest biomass production. The performance of ryegrass exudates was generally lower, and variable responses were observed between bacteria. In addition, P. fluorescens and A. brasilense appeared to respond more efficiently to root exudates than B. subtilis. Finally, we proposed to evaluate the compatibility of each plant-PGPR couple by assigning them a "love match" score, which reflects the ability of root exudates to enhance bacterial rhizocompetence.
Discussion: Taken together, our results provide new insights into the specific selection of PGPR by the plant through their root exudates and may help to select the most effective exudates to promote bioinoculant establishment in the rhizosphere.
期刊介绍:
Frontiers in Microbiology is a leading journal in its field, publishing rigorously peer-reviewed research across the entire spectrum of microbiology. Field Chief Editor Martin G. Klotz at Washington State University is supported by an outstanding Editorial Board of international researchers. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.