皮损型银屑病与角质层神经酰胺谱的改变和屏障功能的降低有关。

IF 3.5 3区 医学 Q1 DERMATOLOGY
Jannik Rousel, Catherine Mergen, Menthe E. Bergmans, Naomi B. Klarenbeek, Tessa Niemeyer-van der Kolk, Martijn B. A. van Doorn, Joke A. Bouwstra, Robert Rissmann, the Next-Generation ImmunoDermatology Consortium (NGID)
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引用次数: 0

摘要

银屑病是一种与皮肤屏障受损有关的炎症性皮肤病。皮肤屏障功能取决于环绕角质层中角质细胞的细胞外脂质基质。神经酰胺是这种基质的重要组成部分。角质层神经酰胺谱的改变与屏障功能障碍直接相关,可能是银屑病屏障受损的潜在因素。在这项研究中,我们调查了银屑病患者的神经酰胺谱和屏障功能。通过液相色谱-质谱联用技术对 26 名患者和 10 名健康对照者的病变和非病变皮肤进行了深入的神经酰胺脂质组学分析。通过测量经表皮失水来评估屏障功能。与对照组和非病变皮肤相比,病变皮肤的神经酰胺总量明显减少,神经酰胺亚类组成也发生了显著变化。此外,病变皮肤中单不饱和神经酰胺的比例明显增加,神经酰胺链的平均长度明显减少。总之,与对照组相比,病变皮肤的神经酰胺组成明显不同,而非病变皮肤则没有。重要的是,病损银屑病皮肤屏障功能的降低与神经酰胺谱的改变相关,这突出了它们之间的相互依存关系。通过对相隔两周的参数进行评估,我们能够强调这些发现的可重复性,从而进一步证实了这种联系。总之,我们的研究表明,在银屑病病变皮肤中可以观察到神经酰胺谱的变化和屏障受损,而且仅限于病变皮肤。它们之间的直接关联为同时观察到的屏障功能障碍提供了进一步的机理依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Lesional Psoriasis is Associated With Alterations in the Stratum Corneum Ceramide Profile and Concomitant Decreases in Barrier Function

Lesional Psoriasis is Associated With Alterations in the Stratum Corneum Ceramide Profile and Concomitant Decreases in Barrier Function

Psoriasis is an inflammatory skin disease associated with an impaired skin barrier. The skin barrier function is dependent on the extracellular lipid matrix which surrounds the corneocytes in the stratum corneum. Ceramides comprise essential components of this matrix. Alterations in the stratum corneum ceramide profile have been directly linked to barrier dysfunction and might be an underlying factor of the barrier impairment in psoriasis. In this study, we investigated the ceramide profile and barrier function in psoriasis. Lesional and non-lesional skin of 26 patients and 10 healthy controls were analysed using in-depth ceramide lipidomics by liquid chromatography-mass spectrometry. Barrier function was assessed by measuring transepidermal water loss. Lesional skin showed a significant decrease in the abundance of total ceramides with significant alterations in the ceramide subclass composition compared to control and non-lesional skin. Additionally, the percentage of monounsaturated ceramides was significantly increased, and the average ceramide chain length significantly decreased in lesional skin. Altogether, this resulted in a markedly different profile compared to controls for lesional skin, but not for non-lesional skin. Importantly, the reduced barrier function in lesional psoriasis correlated to alterations in the ceramide profile, highlighting their interdependence. By assessing the parameters 2 weeks apart, we are able to highlight the reproducibility of these findings, which further affirms this connection. To conclude, we show that changes in the ceramide profile and barrier impairment are observed in, and limited to, lesional psoriatic skin. Their direct correlation provides a further mechanistic basis for the concomitantly observed impairment of barrier dysfunction.

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来源期刊
Experimental Dermatology
Experimental Dermatology 医学-皮肤病学
CiteScore
6.70
自引率
5.60%
发文量
201
审稿时长
2 months
期刊介绍: Experimental Dermatology provides a vehicle for the rapid publication of innovative and definitive reports, letters to the editor and review articles covering all aspects of experimental dermatology. Preference is given to papers of immediate importance to other investigators, either by virtue of their new methodology, experimental data or new ideas. The essential criteria for publication are clarity, experimental soundness and novelty. Letters to the editor related to published reports may also be accepted, provided that they are short and scientifically relevant to the reports mentioned, in order to provide a continuing forum for discussion. Review articles represent a state-of-the-art overview and are invited by the editors.
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