猪胚胎前后轴形成过程中打破对称的瞬态结构域网络。

IF 2 3区 生物学 Q2 ANATOMY & MORPHOLOGY
Ruben Plöger, Nikoloz Tsikolia, Christoph Viebahn
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引用次数: 0

摘要

打破辐射对称以形成前后轴是脊椎动物胃形成的关键发育步骤之一,它是通过一系列由形态学或基因表达所定义的瞬时区域建立起来的。最近在兔子身上发现的三个这样的区域被解释为轴形成的 "三锚点模型 "的一部分。为了回答该模型是否普遍适用于哺乳动物的问题,我们在猪体内分析了四个标记基因的动态表达模式:EOMES和PKDCC转录本在前下胚层的表达强度不断下降,而在前条纹域和结节的表达强度则与WNT3一起不断上升;TBX6的表达从最初的中心表达变为仅在原始条纹的后端表达。因此,前条纹域的分子足迹与兔相似,末端结节在物种间共享 TBX6,而前下胚层--模仿猪上胚层的特定衍生和命运--则以 PKDCC 而非 WNT3 为标志。瞬时结构域的分子相似性表明,哺乳动物前后轴的建立机制是一致的,而且可能打破了辐射对称。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A network of transient domains for breaking symmetry during anterior-posterior axis formation in the porcine embryo.

Breaking radial symmetry for anterior-posterior axis formation is one of the key developmental steps of vertebrate gastrulation and is established through a succession of transient domains defined by morphology or gene expression. Three such domains were interpreted recently in the rabbit to be part of a "three-anchor-point model" for axis formation. To answer the question as to whether the model is generally applicable to mammals, the dynamic expression patterns of four marker genes were analyzed in the pig, where gastrulating epiblast forms from half the inner cell mass: EOMES and PKDCC transcripts display decreasing expression intensities in the anterior hypoblast and-together with WNT3-increasing intensity in the anterior streak domain and the node; TBX6 expression changes from an initial central expression to exclusive expression in the posterior extremity of the primitive streak. The anterior streak domain has thus a molecular footprint similar to the one in the rabbit, the end node shares TBX6 between the species, while the anterior hypoblast-mirroring specific porcine epiblast derivation and fate-is marked by PKDCC instead of WNT3. The molecular similarities in transient domains point to conserved mechanisms for establishing the mammalian anterior-posterior axis and, possibly, breaking radial symmetry.

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来源期刊
Developmental Dynamics
Developmental Dynamics 生物-发育生物学
CiteScore
5.10
自引率
8.00%
发文量
116
审稿时长
3-8 weeks
期刊介绍: Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.
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