黑麦油能减轻实验性心肌梗塞的炎症和氧化应激反应。

IF 3.3 2区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Raluca Maria Pop, Emilia Vassilopoulou, Mihaela-Elena Jianu, Ștefan Horia Roșian, Marian Taulescu, Mihai Negru, Crina Bercian, Paul-Mihai Boarescu, Ioana Corina Bocsan, Gavriela Feketea, Veronica Sanda Chedea, Francisc Dulf, Jeanine Cruceru, Alina Elena Pârvu, Anca Dana Buzoianu
{"title":"黑麦油能减轻实验性心肌梗塞的炎症和氧化应激反应。","authors":"Raluca Maria Pop, Emilia Vassilopoulou, Mihaela-Elena Jianu, Ștefan Horia Roșian, Marian Taulescu, Mihai Negru, Crina Bercian, Paul-Mihai Boarescu, Ioana Corina Bocsan, Gavriela Feketea, Veronica Sanda Chedea, Francisc Dulf, Jeanine Cruceru, Alina Elena Pârvu, Anca Dana Buzoianu","doi":"10.1186/s12906-024-04648-2","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>A growing interest in using Nigella sativa oil (NSO) in the prevention or treatment of several cardiovascular diseases has prompted this study. The research aims to investigate the effect of NSO on cardiac damage prevention after long-term administration in induced myocardial infarction (MI) in rats.</p><p><strong>Methods: </strong>NSO was analyzed for its fatty acids composition using gas chromatography-mass spectrometry (GC-MS) analysis and administered in rats before and after isoproterenol (45 mg/kg body weight) induced myocardial infarction. The following parameters were assessed: electrocardiograms, histopathological examination, serum biochemical aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase-myocardial band (CK-MB), serum and heart inflammation (tumor necrosis factor-alpha (TNF), interleukin 1 beta (IL-1b), and interleukin 6 (IL-6)), and tissue oxidative stress (total antioxidant capacity (TAC), total oxidative stress (TOS), nitric oxide (NO), malondialdehyde (MDA), and the total thiols (THIOL)).</p><p><strong>Results: </strong>Linoleic acid (C18:2n-6) and oleic acid (C18:1n-9) were approximately 89% of total fatty acids while palmitic acid (C16:0) was 6.10%. Administration of NSO for 28 days helped in preventing QT and QTc interval prolongation and reduced heart rate (HR), after MI induction. The histological assessment showed improvement in myofibrillary degeneration and necrosis and also better reduced inflammatory process in the groups treated with NSO. In serum, pro-inflammatory cytokines IL-1b and IL-6 were downregulated in chronic conditions (for IL-1b, NSO vs. control was 86.09vs 150.39 pg/mL, and for IL-6 NSO vs. control was 78.00 vs. 184.98 pg/ml). In the heart tissue, the downregulation was observed only for TNF in both acute and chronic conditions (acute NSO vs. control was 132.37 vs. 207.63 pg/mL, and chronic NSO vs. control was 135.83 vs. 183.29 pg/ml). The pro-oxidant parameters TOS, NO, MDA, and OSI, were reduced in the groups treated with NSO only after 14 days of treatment, suggesting that the NSO antioxidant effect is time-dependent.</p><p><strong>Conclusions: </strong>NSO administration might have a favourable impact on the regulation of oxidative stress and inflammation processes after MI induction in rats, and it is worth considering its administration as an adjuvant treatment.</p>","PeriodicalId":9128,"journal":{"name":"BMC Complementary Medicine and Therapies","volume":"24 1","pages":"362"},"PeriodicalIF":3.3000,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459993/pdf/","citationCount":"0","resultStr":"{\"title\":\"Nigella sativa oil attenuates inflammation and oxidative stress in experimental myocardial infarction.\",\"authors\":\"Raluca Maria Pop, Emilia Vassilopoulou, Mihaela-Elena Jianu, Ștefan Horia Roșian, Marian Taulescu, Mihai Negru, Crina Bercian, Paul-Mihai Boarescu, Ioana Corina Bocsan, Gavriela Feketea, Veronica Sanda Chedea, Francisc Dulf, Jeanine Cruceru, Alina Elena Pârvu, Anca Dana Buzoianu\",\"doi\":\"10.1186/s12906-024-04648-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>A growing interest in using Nigella sativa oil (NSO) in the prevention or treatment of several cardiovascular diseases has prompted this study. The research aims to investigate the effect of NSO on cardiac damage prevention after long-term administration in induced myocardial infarction (MI) in rats.</p><p><strong>Methods: </strong>NSO was analyzed for its fatty acids composition using gas chromatography-mass spectrometry (GC-MS) analysis and administered in rats before and after isoproterenol (45 mg/kg body weight) induced myocardial infarction. The following parameters were assessed: electrocardiograms, histopathological examination, serum biochemical aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase-myocardial band (CK-MB), serum and heart inflammation (tumor necrosis factor-alpha (TNF), interleukin 1 beta (IL-1b), and interleukin 6 (IL-6)), and tissue oxidative stress (total antioxidant capacity (TAC), total oxidative stress (TOS), nitric oxide (NO), malondialdehyde (MDA), and the total thiols (THIOL)).</p><p><strong>Results: </strong>Linoleic acid (C18:2n-6) and oleic acid (C18:1n-9) were approximately 89% of total fatty acids while palmitic acid (C16:0) was 6.10%. Administration of NSO for 28 days helped in preventing QT and QTc interval prolongation and reduced heart rate (HR), after MI induction. The histological assessment showed improvement in myofibrillary degeneration and necrosis and also better reduced inflammatory process in the groups treated with NSO. In serum, pro-inflammatory cytokines IL-1b and IL-6 were downregulated in chronic conditions (for IL-1b, NSO vs. control was 86.09vs 150.39 pg/mL, and for IL-6 NSO vs. control was 78.00 vs. 184.98 pg/ml). In the heart tissue, the downregulation was observed only for TNF in both acute and chronic conditions (acute NSO vs. control was 132.37 vs. 207.63 pg/mL, and chronic NSO vs. control was 135.83 vs. 183.29 pg/ml). The pro-oxidant parameters TOS, NO, MDA, and OSI, were reduced in the groups treated with NSO only after 14 days of treatment, suggesting that the NSO antioxidant effect is time-dependent.</p><p><strong>Conclusions: </strong>NSO administration might have a favourable impact on the regulation of oxidative stress and inflammation processes after MI induction in rats, and it is worth considering its administration as an adjuvant treatment.</p>\",\"PeriodicalId\":9128,\"journal\":{\"name\":\"BMC Complementary Medicine and Therapies\",\"volume\":\"24 1\",\"pages\":\"362\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2024-10-07\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11459993/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"BMC Complementary Medicine and Therapies\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s12906-024-04648-2\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"BMC Complementary Medicine and Therapies","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s12906-024-04648-2","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}
引用次数: 0

摘要

背景:人们对使用黑麦油(NSO)预防或治疗多种心血管疾病的兴趣与日俱增,这促使了本研究的开展。本研究旨在探讨 NSO 在诱发大鼠心肌梗死(MI)后长期服用对预防心脏损伤的作用:方法:使用气相色谱-质谱分析法(GC-MS)分析 NSO 的脂肪酸组成,并在异丙肾上腺素(45 毫克/千克体重)诱发心肌梗塞前后给大鼠服用 NSO。对以下参数进行了评估:心电图、组织病理学检查、血清生化天冬氨酸氨基转移酶 (AST)、丙氨酸氨基转移酶 (ALT)、肌酸激酶-心肌带 (CK-MB)、血清和心脏炎症(肿瘤坏死因子-α (TNF)、1β(IL-1b)和白细胞介素 6(IL-6))以及组织氧化应激(总抗氧化能力(TAC)、总氧化应激(TOS)、一氧化氮(NO)、丙二醛(MDA)和总硫醇(THIOL))。研究结果亚油酸(C18:2n-6)和油酸(C18:1n-9)约占总脂肪酸的 89%,而棕榈酸(C16:0)占 6.10%。在诱发心肌缺血后,连续 28 天服用 NSO 有助于防止 QT 和 QTc 间期延长并降低心率(HR)。组织学评估显示,NSO 治疗组的肌纤维变性和坏死情况有所改善,炎症过程也有所减轻。在血清中,促炎细胞因子IL-1b和IL-6在慢性情况下被下调(对于IL-1b,NSO与对照组相比为86.09 vs. 150.39 pg/ml;对于IL-6,NSO与对照组相比为78.00 vs. 184.98 pg/ml)。在心脏组织中,只有 TNF 在急性和慢性条件下都出现了下调(急性 NSO 与对照组相比为 132.37 与 207.63 微微克/毫升,慢性 NSO 与对照组相比为 135.83 与 183.29 微微克/毫升)。治疗14天后,NSO治疗组的促氧化剂参数TOS、NO、MDA和OSI才有所降低,这表明NSO的抗氧化作用具有时间依赖性:结论:给予 NSO 可能会对诱导 MI 后大鼠氧化应激和炎症过程的调节产生有利影响,值得考虑将其作为一种辅助治疗手段。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nigella sativa oil attenuates inflammation and oxidative stress in experimental myocardial infarction.

Background: A growing interest in using Nigella sativa oil (NSO) in the prevention or treatment of several cardiovascular diseases has prompted this study. The research aims to investigate the effect of NSO on cardiac damage prevention after long-term administration in induced myocardial infarction (MI) in rats.

Methods: NSO was analyzed for its fatty acids composition using gas chromatography-mass spectrometry (GC-MS) analysis and administered in rats before and after isoproterenol (45 mg/kg body weight) induced myocardial infarction. The following parameters were assessed: electrocardiograms, histopathological examination, serum biochemical aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase-myocardial band (CK-MB), serum and heart inflammation (tumor necrosis factor-alpha (TNF), interleukin 1 beta (IL-1b), and interleukin 6 (IL-6)), and tissue oxidative stress (total antioxidant capacity (TAC), total oxidative stress (TOS), nitric oxide (NO), malondialdehyde (MDA), and the total thiols (THIOL)).

Results: Linoleic acid (C18:2n-6) and oleic acid (C18:1n-9) were approximately 89% of total fatty acids while palmitic acid (C16:0) was 6.10%. Administration of NSO for 28 days helped in preventing QT and QTc interval prolongation and reduced heart rate (HR), after MI induction. The histological assessment showed improvement in myofibrillary degeneration and necrosis and also better reduced inflammatory process in the groups treated with NSO. In serum, pro-inflammatory cytokines IL-1b and IL-6 were downregulated in chronic conditions (for IL-1b, NSO vs. control was 86.09vs 150.39 pg/mL, and for IL-6 NSO vs. control was 78.00 vs. 184.98 pg/ml). In the heart tissue, the downregulation was observed only for TNF in both acute and chronic conditions (acute NSO vs. control was 132.37 vs. 207.63 pg/mL, and chronic NSO vs. control was 135.83 vs. 183.29 pg/ml). The pro-oxidant parameters TOS, NO, MDA, and OSI, were reduced in the groups treated with NSO only after 14 days of treatment, suggesting that the NSO antioxidant effect is time-dependent.

Conclusions: NSO administration might have a favourable impact on the regulation of oxidative stress and inflammation processes after MI induction in rats, and it is worth considering its administration as an adjuvant treatment.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
BMC Complementary Medicine and Therapies
BMC Complementary Medicine and Therapies INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
6.10
自引率
2.60%
发文量
300
审稿时长
19 weeks
期刊介绍:
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信