评估谷胱甘肽和初榨椰子油新型纳米颗粒制剂在四氯化碳诱发肝衰竭实验模型中的治疗潜力。

IF 2.8 3区 医学 Q2 PHARMACOLOGY & PHARMACY
Essmat A H Allam, Madeha H A Darwish, Nasser S Abou Khalil, Shimaa H A Abd El-Baset, Mohamed Abd El-Aal, Ahmed Elrawy, Ahmed A N Ahmed, Mahmoud S Sabra
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引用次数: 0

摘要

背景:急性肝功能衰竭(ALF)是一种严重的疾病,其特点是肝功能迅速失调,导致很高的死亡率。目前的治疗方法有限,主要是支持性治疗,通常需要进行肝移植。本研究调查了谷胱甘肽(GSH)和初榨椰子油(VCO)的新型纳米颗粒配方单独或联合使用的潜力,以提高口服四氯化碳(CCl4)诱导的大鼠 ALF 模型的治疗效果:研究采用成年雄性白化大鼠,分为十组,通过单次口服四氯化碳诱导 ALF。在七天内采用不同的治疗方案,包括常规和纳米颗粒形式的 GSH 和 VCO 及其组合。通过对肝功能标志物、氧化应激指标、炎症生物标志物的生化分析以及组织病理学检查来评估治疗效果。纳米颗粒采用既定方法合成,并采用表征技术确保其质量和特性:结果:纳米颗粒制剂明显改善了肝功能,血清中丙氨酸氨基转移酶和天门冬氨酸氨基转移酶水平降低,丙二醛等氧化应激标志物减少。此外,它们还降低了肿瘤坏死因子α和白细胞介素-1β等炎症指标。组织学分析表明,与对照组相比,治疗组的肝细胞坏死和炎症有所减少。此外,免疫组化分析还检测到核因子卡巴 B 的减少:研究结果表明,GSH 和 VCO 的纳米颗粒混合物能有效减轻 ALF 的肝损伤。结论:研究结果表明,GSH 和 VCO 的纳米颗粒混合物能有效减轻 ALF 的肝损伤,这为改善肝脏再生和保护提供了一种很有前景的药物治疗方法。这种创新策略可能为治疗 ALF 的新疗法铺平道路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evaluation of the therapeutic potential of novel nanoparticle formulations of glutathione and virgin coconut oil in an experimental model of carbon tetrachloride-induced liver failure.

Background: Acute liver failure (ALF) is a critical condition characterized by rapid liver dysfunction, leading to high mortality rates. Current treatments are limited, primarily supportive, and often require liver transplantation. This study investigates the potential of a novel nanoparticle formulation of glutathione (GSH) and virgin coconut oil (VCO) alone and in combination to enhance therapeutic outcomes in a rat model of ALF induced by orogastric carbon tetrachloride (CCl4).

Methods: The study employed adult male Albino rats divided into ten groups, with ALF induced via a single oral dose of CCl4. Various treatment regimens were administered over seven days, including conventional and nanoparticle forms of GSH and VCO and their combinations. The efficacy of treatments was evaluated through biochemical analysis of liver function markers, oxidative stress indicators, inflammatory biomarkers, and histopathological examinations. Nanoparticles were synthesized using established methods, and characterization techniques were employed to ensure their quality and properties.

Results: The nanoparticle formulations significantly improved liver function, as indicated by reduced serum levels of alanine aminotransferase and aspartate aminotransferase, alongside decreased oxidative stress markers such as malondialdehyde. Furthermore, they reduced tumor necrosis factor alpha and interleukin-1 beta inflammatory markers. Histological analysis revealed reduced hepatocellular necrosis and inflammation in treated groups compared to controls. Also, decreased nuclear factor-kappa B was detected by immunohistochemical analysis.

Conclusion: The findings show that the nanoparticle mixture of GSH and VCO effectively reduces liver damage in ALF. This suggests a promising drug-based approach for improving liver regeneration and protection. This innovative strategy may pave the way for new therapeutic interventions in the management of ALF.

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来源期刊
BMC Pharmacology & Toxicology
BMC Pharmacology & Toxicology PHARMACOLOGY & PHARMACYTOXICOLOGY&nb-TOXICOLOGY
CiteScore
4.80
自引率
0.00%
发文量
87
审稿时长
12 weeks
期刊介绍: BMC Pharmacology and Toxicology is an open access, peer-reviewed journal that considers articles on all aspects of chemically defined therapeutic and toxic agents. The journal welcomes submissions from all fields of experimental and clinical pharmacology including clinical trials and toxicology.
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