在植物病原真菌 Alternaria tenuissima 中发现的新型丝裂病毒的完整基因组序列。

IF 2.5 4区 医学 Q3 VIROLOGY
Tongyu Mu, Zhonglei Wang, Zhijun Liu, Xuehong Wu
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引用次数: 0

摘要

本研究在导致麝香瓜叶枯病的植物病原真菌Alternaria tenuissima菌株YQ-2-1中鉴定出一种新型正义单链RNA(+ ssRNA)真菌病毒Alternaria tenuissima mitovirus 1(AtMV1)。AtMV1 的基因组是一个长度为 3013 nt 的单一 RNA 分子,A+U 含量为 66.58%,包含一个使用真菌线粒体遗传密码的开放阅读框(ORF)。据预测,该 ORF 编码一个 313 氨基酸的 RNA 依赖性 RNA 聚合酶(RdRp),分子质量为 35.48 kDa,其中包含六个保守基序,基序 IV 中包含高度保守的 GDD 三肽。据预测,5'和 3'非翻译区可折叠成茎环和泛柄二级结构。BLASTp 搜索结果显示,AtMV1 的 RdRp 氨基酸序列与丝状病毒科 Duamitovirus 属的四川丝状病毒 30 的序列相似度最高(51.04%)。基于RdRp的aa序列的系统进化分析表明,AtMV1是杜米妥病毒属的一个新成员。据我们所知,这是首次报道感染 A. tenuissima 的新丝状病毒的完整基因组序列。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Complete genome sequence of a novel mitovirus identified in the phytopathogenic fungus Alternaria tenuissima

In this study, a novel positive-sense single-stranded RNA (+ ssRNA) mycovirus, Alternaria tenuissima mitovirus 1 (AtMV1), was identified in Alternaria tenuissima strain YQ-2-1, a phytopathogenic fungus causing leaf blight on muskmelon. The genome of AtMV1 is a single RNA molecule that is 3013 nt in length with an A + U content of 66.58% and contains a single open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF was predicted to encode a 313-amino-acid RNA-dependent RNA polymerase (RdRp) with a molecular mass of 35.48 kDa, which contains six conserved motifs with the highly conserved GDD tripeptide in motif IV. The 5ʹ and 3ʹ untranslated regions were predicted to fold into stem-loop and panhandle secondary structures. The results of a BLASTp search revealed that the amino acid (aa) sequence of RdRp of AtMV1 shared the highest sequence similarity (51.04% identity) with that of Sichuan mito-like virus 30, a member of the genus Duamitovirus within the family Mitoviridae. Phylogenetic analysis based on the aa sequence of the RdRp suggested that AtMV1 is a novel member of the genus Duamitovirus. To our knowledge, this is the first report of the complete genome sequence of a new mitovirus infecting A. tenuissima.

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来源期刊
Archives of Virology
Archives of Virology 医学-病毒学
CiteScore
5.10
自引率
7.40%
发文量
324
审稿时长
4.5 months
期刊介绍: Archives of Virology publishes original contributions from all branches of research on viruses, virus-like agents, and virus infections of humans, animals, plants, insects, and bacteria. Coverage spans a broad spectrum of topics, from descriptions of newly discovered viruses, to studies of virus structure, composition, and genetics, to studies of virus interactions with host cells, organisms and populations. Studies employ molecular biologic, molecular genetics, and current immunologic and epidemiologic approaches. Contents include studies on the molecular pathogenesis, pathophysiology, and genetics of virus infections in individual hosts, and studies on the molecular epidemiology of virus infections in populations. Also included are studies involving applied research such as diagnostic technology development, monoclonal antibody panel development, vaccine development, and antiviral drug development.Archives of Virology wishes to publish obituaries of recently deceased well-known virologists and leading figures in virology.
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