D-木糖通过靶向巨噬细胞表达的 LYZ 基因改善非酒精性脂肪肝。

IF 1.8 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Guoxiang Liu, Sreemoy Kanti Das
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引用次数: 0

摘要

本研究调查了天然糖类 D- 木糖对非酒精性脂肪肝(NAFLD)的治疗效果,重点关注巨噬细胞中溶菌酶基因(LYZ)的表达。利用非酒精性脂肪肝的单细胞数据集 GSE136103,研究人员使用 R 中的 Seurat 软件包分析了巨噬细胞群和其他群体,同时使用 limma 软件包对非酒精性脂肪肝数据集 GSE61260 进行了差异分析。研究人员采用了体外和体内模型,包括细胞培养、小鼠模型、RT-qPCR、Western 印迹、ELISA 和组织病理学分析,来研究 D-Xylose 对脂质积累、LYZ 表达、血脂水平和炎症反应的影响。研究发现,在游离脂肪酸(FFA)处理的细胞和小鼠肝组织中,LYZ 的表达明显上调,而在 D- 木糖干预后,LYZ 的表达随之下降。使用木糖和氨氯地平治疗后,脂质积累明显减少,甘油三酯和胆固醇水平降低就是证明。与氨氯地平相比,D-木糖对脂质代谢的改善更大。此外,D-木糖还能显著减轻炎症反应,降低炎症标志物的水平,如 IL1R、IL6、MYS8、TNF、NF-κB 和 IL-1。此外,服用 D- 木糖还能显著降低肝脏重量和肝脏指数,并对血清肝功能和血脂水平产生积极影响。研究结果表明,D-木糖可通过靶向巨噬细胞中LYZ的表达,从而调节脂质代谢和炎症反应,对非酒精性脂肪肝起到治疗干预作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
D-Xylose Ameliorates Non-Alcoholic Fatty Liver Disease by Targeting Macrophage-expressed LYZ Gene.

This study investigates the therapeutic effects of D-Xylose, a natural sugar, on non-alcoholic fatty liver disease (NAFLD), focusing on the expression of the lysozyme gene (LYZ) in macrophages. Using the single-cell dataset GSE136103 for NAFLD, researchers analyzed macrophage populations and other groups utilizing the Seurat package in R, while a differential analysis was performed on the NAFLD dataset GSE61260 using the limma package. Both in vitro and in vivo models, including cell culture, mouse models, RT-qPCR, Western blot, ELISA, and histopathological analyses, were employed to examine the effect of D-Xylose on lipid accumulation, LYZ expression, blood lipid levels, and inflammatory responses. The study found a significant upregulation of LYZ in free fatty acid (FFA)-treated cells and mouse liver tissues, with a subsequent reduction after D-Xylose intervention. Treatment with D-Xylose and Amlodipine led to a notable decrease in lipid accumulation, as evidenced by reduced triglyceride and cholesterol levels. D-Xylose demonstrated a greater improvement in lipid metabolism than Amlodipine. Additionally, D-Xylose significantly mitigated inflammatory responses, reducing levels of inflammatory markers such as IL1R, IL6, MYS8, TNF, NF-κB, and IL-1. Furthermore, D-Xylose administration significantly reduced liver weight and liver index, with a positive impact on serum liver function and blood lipid levels. The findings suggest that D-Xylose could be a therapeutic intervention for NAFLD by targeting LYZ expression in macrophages, thereby modulating lipid metabolism and inflammatory responses.

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来源期刊
Cell Biochemistry and Biophysics
Cell Biochemistry and Biophysics 生物-生化与分子生物学
CiteScore
4.40
自引率
0.00%
发文量
72
审稿时长
7.5 months
期刊介绍: Cell Biochemistry and Biophysics (CBB) aims to publish papers on the nature of the biochemical and biophysical mechanisms underlying the structure, control and function of cellular systems The reports should be within the framework of modern biochemistry and chemistry, biophysics and cell physiology, physics and engineering, molecular and structural biology. The relationship between molecular structure and function under investigation is emphasized. Examples of subject areas that CBB publishes are: · biochemical and biophysical aspects of cell structure and function; · interactions of cells and their molecular/macromolecular constituents; · innovative developments in genetic and biomolecular engineering; · computer-based analysis of tissues, cells, cell networks, organelles, and molecular/macromolecular assemblies; · photometric, spectroscopic, microscopic, mechanical, and electrical methodologies/techniques in analytical cytology, cytometry and innovative instrument design For articles that focus on computational aspects, authors should be clear about which docking and molecular dynamics algorithms or software packages are being used as well as details on the system parameterization, simulations conditions etc. In addition, docking calculations (virtual screening, QSAR, etc.) should be validated either by experimental studies or one or more reliable theoretical cross-validation methods.
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