肿瘤相关 miRNA 的现场可视化检测：使用 Ru@TiO2 作为过氧化物酶样纳米酶

IF 5.3 2区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Nano Materials Pub Date : 2024-10-09 DOI:10.1021/acs.analchem.4c03922

Limei Zhang, Xun He, Hao Bai, Xingzhi Yu, Peilin Wu, Zhengwei Cai, Yuchun Ren, Yongsong Luo, Weihua Zhuang, Jie Hu, Binwu Ying, Fengming Luo, Yongchao Yao, Xuping Sun, Wenchuang Hu

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引用次数: 0

摘要

准确诊断侵袭性强的致命肿瘤对于有效治疗和改善患者预后至关重要，而微小RNA（miRNA）因其在肿瘤发生、发展和转移中的作用而成为重要的生物标记物。在此，我们提出了一种现场可视化比色法，利用钌纳米粒子装饰的二氧化钛纳米带（Ru@TiO2）作为过氧化物酶样（POD）纳米酶，检测与肿瘤相关的 miRNA。值得注意的是，Ru@TiO2 纳米酶可通过其类过氧化物酶活性催化发色底物的氧化，而在滚圈扩增过程中产生的焦磷酸可有效抑制该活性，从而实现通过可见比色读数检测 miRNA。这种方法可对稀释人血清中的 miRNA 进行高灵敏度和特异性检测，检测限为 100 pM。它在临床诊断和生物学研究方面显示出巨大的潜力，为早期癌症诊断和分子诊断提供了一种前景广阔的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

On-Site Visualization Assay for Tumor-Associated miRNAs: Using Ru@TiO2 as a Peroxidase-like Nanozyme

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On-Site Visualization Assay for Tumor-Associated miRNAs: Using Ru@TiO2 as a Peroxidase-like Nanozyme

Accurate diagnosis of highly aggressive and deadly tumors is essential for effective treatment and improved patient outcomes, and microRNAs (miRNAs) have emerged as crucial biomarkers for their roles in tumor initiation, progression, and metastasis. Herein, we present an on-site visualization colorimetric assay for tumor-associated miRNAs using ruthenium nanoparticle decorated titanium dioxide nanoribbon (Ru@TiO₂) as a peroxidase-like (POD) nanozyme. Remarkably, the Ru@TiO₂ nanozyme can catalyze the oxidation of chromogenic substrates through its POD-like activity, which is effectively inhibited by pyrophosphate generated during the rolling circle amplification process, thereby enabling miRNA detection through a visible colorimetric readout. This approach provides a highly sensitive and specificity assay for miRNAs in diluted human serum with a detection limit of 100 pM. It shows great potential for clinical diagnostics and biological research, offering a promising tool for early cancer diagnosis and molecular diagnostics.

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来源期刊

ACS Applied Nano Materials Multiple-

CiteScore

8.30

自引率

3.40%

发文量

1601

期刊介绍： ACS Applied Nano Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics and biology relevant to applications of nanomaterials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important applications of nanomaterials.