淡水中硫和硫代硫酸盐驱动的化学自养反硝化过程中产生的一氧化二氮的同位素特征

IF 3.8 1区地球科学 Q1 LIMNOLOGY

Limnology and Oceanography Pub Date : 2024-10-06 DOI:10.1002/lno.12692

Shengjie Li, Shuo Wang, Yunmeng Pang, Guodong Ji

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引用次数: 0

摘要

化学自养反硝化作用在水生环境中脱氮和形成温室气体（N2O）方面发挥着重要作用。天然稳定同位素有助于在实地研究中追踪氮源和确定生物地球化学过程。然而，迄今为止还没有研究过化学自养反硝化过程中产生的 N2O 的同位素特征。在本研究中，我们分析了淡水湖中依赖硫和硫代硫酸盐的反硝化富集物中硝酸盐和一氧化二氮的同位素特征。然而，与异养反硝化作用相比，化学自养反硝化作用产生的 N2O 的 δ15N 和 δ18O 值较低，而位点偏好（SP = δ15Nα-δ15Nβ）值较高。SP 值约为 5.1‰，是检测由不同硫形式驱动的化能自养反硝化作用的特征值。δ18O随特定电子供体的变化而变化，在依赖硫和硫代硫酸盐的反硝化作用中分别约为20‰和40‰。在硫依赖性反硝化过程中，Burkholderiaceae种群的一氧化氮还原酶可能会调节这种独特的N2O同位素特性；在硫代硫酸依赖性反硝化过程中，Sulfurovum种群的一氧化氮还原酶可能会调节这种独特的N2O同位素特性。这些发现增进了我们对一氧化二氮产生过程的了解，并对以更高的时空分辨率预测一氧化二氮排放具有重要意义。

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Isotopic signature of N2O produced during sulfur‐ and thiosulfate‐driven chemoautotrophic denitrification in freshwaters

Chemoautotrophic denitrification plays an important role in nitrogen removal and the formation of a greenhouse gas (N2O) in aquatic environments. Natural stable isotopes support the tracing of nitrogen sources and the identification of biogeochemical processes in field research. However, the isotopic characteristics of N2O produced during chemoautotrophic denitrification have not been investigated so far. In this study, we analyzed isotopic signatures of nitrate and N2O in sulfur‐ and thiosulfate‐dependent denitrifying enrichments obtained from freshwater lakes. Chemoautotrophic denitrification exhibited a nitrate isotope pattern similar to heterotrophic denitrification: the 18ε/15ε‐nitrate followed a ratio close to 1. However, chemoautotrophic denitrification produced N2O with lower δ15N, δ18O, and higher site preference (SP = δ15Nα‐δ15Nβ) values, compared to heterotrophic denitrification. The SP value, approximately 5.1‰, was characteristic in detecting chemoautotrophic denitrification driven by different sulfur forms. δ18O varied with specific electron donors, around 20‰ and 40‰ during sulfur‐ and thiosulfate‐dependent denitrification, respectively. The unique N2O isotope characteristics were likely regulated by nitric oxide reductases of Burkholderiaceae populations during sulfur‐dependent denitrification and Sulfurovum during thiosulfate‐dependent denitrification. These findings improve our understanding of N2O production processes and have important implications for predicting N2O emissions at a greater spatial and temporal resolution.

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来源期刊

Limnology and Oceanography 地学-海洋学

CiteScore

8.80

自引率

6.70%

发文量

254

审稿时长

3 months

期刊介绍： Limnology and Oceanography (L&O; print ISSN 0024-3590, online ISSN 1939-5590) publishes original articles, including scholarly reviews, about all aspects of limnology and oceanography. The journal''s unifying theme is the understanding of aquatic systems. Submissions are judged on the originality of their data, interpretations, and ideas, and on the degree to which they can be generalized beyond the particular aquatic system examined. Laboratory and modeling studies must demonstrate relevance to field environments; typically this means that they are bolstered by substantial "real-world" data. Few purely theoretical or purely empirical papers are accepted for review.