Yu Liu, Thore Rohwerder, Maria L. Bonatelli, Theda von Postel, Sabine Kleinsteuber, Lorenz Adrian, Chang Ding
{"title":"从 Shinella 菌株看污水处理厂中用于安赛蜜降解的新型硫酸酯酶","authors":"Yu Liu, Thore Rohwerder, Maria L. Bonatelli, Theda von Postel, Sabine Kleinsteuber, Lorenz Adrian, Chang Ding","doi":"10.1021/acs.est.4c02283","DOIUrl":null,"url":null,"abstract":"The artificial sweetener acesulfame is a persistent pollutant in wastewater worldwide. So far, only a few bacterial isolates were recently found to degrade acesulfame efficiently. In <i>Bosea</i> and <i>Chelatococcus</i> strains, a Mn<sup>2+</sup>-dependent metallo-β-lactamase-type sulfatase and an amidase signature family enzyme catalyze acesulfame hydrolysis via acetoacetamide-<i>N</i>-sulfonate to acetoacetate. Here, we describe a new acesulfame sulfatase in <i>Shinella</i> strains isolated from wastewater treatment plants in Germany. Their genomes do not encode the Mn<sup>2+</sup>-dependent sulfatase. Instead, a formylglycine-dependent sulfatase gene was found, together with the acetoacetamide-<i>N</i>-sulfonate amidase gene on a plasmid shared by all known acesulfame-degrading <i>Shinella</i> strains. Heterologous expression, proteomics, and size exclusion chromatography corroborated the physiological function of the <i>Shinella</i> sulfatase in acesulfame hydrolysis. Since both acesulfame sulfatase types are absent in other bacterial genomes or metagenome-assembled genomes, we surveyed 73 tera base pairs of wastewater-associated metagenome raw data sets. <i>Bosea</i>/<i>Chelatococcus</i> sulfatase gene signatures were regularly found from 2013, particularly in North America, Europe, and East Asia, whereas <i>Shinella</i> sulfatase gene signatures were first detected in 2020. Moreover, signatures for the <i>Shinella</i> sulfatase and amidase genes co-occur only in six data sets from China, Finland, and Mexico, suggesting that the <i>Shinella</i> genes were enriched or introduced quite recently in wastewater treatment facilities.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":null,"pages":null},"PeriodicalIF":10.8000,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Novel Sulfatase for Acesulfame Degradation in Wastewater Treatment Plants as Evidenced from Shinella Strains\",\"authors\":\"Yu Liu, Thore Rohwerder, Maria L. Bonatelli, Theda von Postel, Sabine Kleinsteuber, Lorenz Adrian, Chang Ding\",\"doi\":\"10.1021/acs.est.4c02283\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"The artificial sweetener acesulfame is a persistent pollutant in wastewater worldwide. So far, only a few bacterial isolates were recently found to degrade acesulfame efficiently. In <i>Bosea</i> and <i>Chelatococcus</i> strains, a Mn<sup>2+</sup>-dependent metallo-β-lactamase-type sulfatase and an amidase signature family enzyme catalyze acesulfame hydrolysis via acetoacetamide-<i>N</i>-sulfonate to acetoacetate. Here, we describe a new acesulfame sulfatase in <i>Shinella</i> strains isolated from wastewater treatment plants in Germany. Their genomes do not encode the Mn<sup>2+</sup>-dependent sulfatase. Instead, a formylglycine-dependent sulfatase gene was found, together with the acetoacetamide-<i>N</i>-sulfonate amidase gene on a plasmid shared by all known acesulfame-degrading <i>Shinella</i> strains. Heterologous expression, proteomics, and size exclusion chromatography corroborated the physiological function of the <i>Shinella</i> sulfatase in acesulfame hydrolysis. 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A Novel Sulfatase for Acesulfame Degradation in Wastewater Treatment Plants as Evidenced from Shinella Strains
The artificial sweetener acesulfame is a persistent pollutant in wastewater worldwide. So far, only a few bacterial isolates were recently found to degrade acesulfame efficiently. In Bosea and Chelatococcus strains, a Mn2+-dependent metallo-β-lactamase-type sulfatase and an amidase signature family enzyme catalyze acesulfame hydrolysis via acetoacetamide-N-sulfonate to acetoacetate. Here, we describe a new acesulfame sulfatase in Shinella strains isolated from wastewater treatment plants in Germany. Their genomes do not encode the Mn2+-dependent sulfatase. Instead, a formylglycine-dependent sulfatase gene was found, together with the acetoacetamide-N-sulfonate amidase gene on a plasmid shared by all known acesulfame-degrading Shinella strains. Heterologous expression, proteomics, and size exclusion chromatography corroborated the physiological function of the Shinella sulfatase in acesulfame hydrolysis. Since both acesulfame sulfatase types are absent in other bacterial genomes or metagenome-assembled genomes, we surveyed 73 tera base pairs of wastewater-associated metagenome raw data sets. Bosea/Chelatococcus sulfatase gene signatures were regularly found from 2013, particularly in North America, Europe, and East Asia, whereas Shinella sulfatase gene signatures were first detected in 2020. Moreover, signatures for the Shinella sulfatase and amidase genes co-occur only in six data sets from China, Finland, and Mexico, suggesting that the Shinella genes were enriched or introduced quite recently in wastewater treatment facilities.
期刊介绍:
Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences.
Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.