利用黑曲霉在 YEPB 培养基中发酵生产唑萘酮染料。

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-04 DOI:10.1007/s13205-024-04098-0
Muhammad Usman Ahmad, Abeera Ahmad, Sadaf Mutahir, Muhammad Asim Khan, Sikander Ali, Abdulrahman A Almehizia, Kaynat William
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引用次数: 0

摘要

目前的研究重点是利用黑曲霉生产和优化天然黄棕色 Azaphilone 染料。研究人员测试了多种培养基,以确定染料合成的最佳条件。反应混合物的颜色变化证实了黄棕色染料的形成,紫外可见光谱在 450 纳米波长处检测到了染料。研究发现,静置条件比振荡条件更有利于提高染料产量,而喂料-分批发酵比分批发酵更有效。培养 28 天后染料产量达到最大。温度、pH 值和接种物百分比等因素都会影响染料的合成,在静态条件下,温度为 30 °C、pH 值为 7、酵母提取物蛋白胨肉汤(YEPB)培养基中孢子悬浮液含量为 3% 时,染料产量最高(2.5 毫升)。气相色谱-质谱(GC-MS)分析验证了培养滤液中存在 Azaphilone 染料。该染料被成功应用于预处理过的棉布上。这些发现加深了我们对优化真菌染料生产以实现可持续和生态友好型纺织品着色应用的理解。这项研究似乎是首次报道黑僵菌在 YEPB 培养基中生产萘甲萘醌染料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Utilization of Aspergillus niger for the fermentative production of azaphilone dye in YEPB medium.

The current research focuses on the production and optimization of a natural yellowish-brown Azaphilone dye using Aspergillus niger. A variety of culture media were tested to ascertain the best conditions for dye synthesis. The formation of the yellowish-brown dye was confirmed by a color shift in the reaction mixture, and UV-Vis spectroscopy detected the dye at 450 nm. Static conditions were found to be more favorable than shaking for higher dye yields, and fed-batch fermentation was more effective than batch fermentation. Maximum dye production was achieved after 28 days of incubation. Factors such as temperature, pH, and inoculum percentage were shown to influence dye synthesis, with the highest production (2.5 ml) occurring at 30 °C, pH 7, and a 3% spore suspension in yeast extract peptone broth (YEPB) medium under static conditions. Gas chromatography-mass spectrometry (GC-MS) analysis validated the presence of Azaphilone dye in the culture filtrate. The dye was successfully applied to a pretreated cotton cloth. These findings advance our understanding of optimizing fungal dye production for sustainable and eco-friendly textile coloration applications. This study appears to be the first of its kind to report azaphilone dye production by A. niger in the YEPB medium.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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