M Asadipour, M R Ataollahi, K Shams, M Ali-Hassanzadeh, E Martinuzzi, K Kalantar
{"title":"肥胖症患者体内的脂肪蛋白肽（adph 129-137）不是 cd8+ t 细胞的靶抗原。","authors":"M Asadipour, M R Ataollahi, K Shams, M Ali-Hassanzadeh, E Martinuzzi, K Kalantar","doi":"10.4183/aeb.2024.21","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong>In obesity, the infiltration of leukocytes into adipose tissue seems to play a key role in the development of inflammation and insulin resistance. Over-expression of adipophilin (ADPH) in adipose tissue, a protein which regulates lipid droplet structure and formation, has been reported in some studies.</p><p><strong>Objective: </strong>To investigate the role of ADPH 129-137 as a target for CD8+ T-cells in PBMCs of patients with obesity.</p><p><strong>Subjects and methods: </strong>PBMCs were obtained from 9 non-diabetic obese patients and 11 healthy subjects expressing the HLA-A0201 molecule. The ELISPOT assay used to monitor the presence of IFN-γ producing CD8+ T-cells against a HLA class I-restricted epitope derived from Adipophilin (ADPH 129-137) and two control peptides: Flu MP58-66 and Melan-A27-35.</p><p><strong>Results: </strong>The outcomes showed no significant difference between patient group and healthy donors in response to ADPH 129-137.</p><p><strong>Conclusion: </strong>These results demonstrated that ADPH 129-137 peptide possibly does not act as an autoantigen in patients with obesity.</p>","PeriodicalId":50902,"journal":{"name":"Acta Endocrinologica-Bucharest","volume":"20 1","pages":"21-26"},"PeriodicalIF":0.7000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449246/pdf/","citationCount":"0","resultStr":"{\"title\":\"ADIPOPHILIN PEPTIDE (ADPH 129-137) IS NOT A TARGET ANTIGEN FOR CD8<sup>+</sup> T-CELLS IN PATIENTS WITH OBESITY.\",\"authors\":\"M Asadipour, M R Ataollahi, K Shams, M Ali-Hassanzadeh, E Martinuzzi, K Kalantar\",\"doi\":\"10.4183/aeb.2024.21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Context: </strong>In obesity, the infiltration of leukocytes into adipose tissue seems to play a key role in the development of inflammation and insulin resistance. Over-expression of adipophilin (ADPH) in adipose tissue, a protein which regulates lipid droplet structure and formation, has been reported in some studies.</p><p><strong>Objective: </strong>To investigate the role of ADPH 129-137 as a target for CD8+ T-cells in PBMCs of patients with obesity.</p><p><strong>Subjects and methods: </strong>PBMCs were obtained from 9 non-diabetic obese patients and 11 healthy subjects expressing the HLA-A0201 molecule. The ELISPOT assay used to monitor the presence of IFN-γ producing CD8+ T-cells against a HLA class I-restricted epitope derived from Adipophilin (ADPH 129-137) and two control peptides: Flu MP58-66 and Melan-A27-35.</p><p><strong>Results: </strong>The outcomes showed no significant difference between patient group and healthy donors in response to ADPH 129-137.</p><p><strong>Conclusion: </strong>These results demonstrated that ADPH 129-137 peptide possibly does not act as an autoantigen in patients with obesity.</p>\",\"PeriodicalId\":50902,\"journal\":{\"name\":\"Acta Endocrinologica-Bucharest\",\"volume\":\"20 1\",\"pages\":\"21-26\"},\"PeriodicalIF\":0.7000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11449246/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta Endocrinologica-Bucharest\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.4183/aeb.2024.21\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/3 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Endocrinologica-Bucharest","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4183/aeb.2024.21","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/3 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
ADIPOPHILIN PEPTIDE (ADPH 129-137) IS NOT A TARGET ANTIGEN FOR CD8+ T-CELLS IN PATIENTS WITH OBESITY.
Context: In obesity, the infiltration of leukocytes into adipose tissue seems to play a key role in the development of inflammation and insulin resistance. Over-expression of adipophilin (ADPH) in adipose tissue, a protein which regulates lipid droplet structure and formation, has been reported in some studies.
Objective: To investigate the role of ADPH 129-137 as a target for CD8+ T-cells in PBMCs of patients with obesity.
Subjects and methods: PBMCs were obtained from 9 non-diabetic obese patients and 11 healthy subjects expressing the HLA-A0201 molecule. The ELISPOT assay used to monitor the presence of IFN-γ producing CD8+ T-cells against a HLA class I-restricted epitope derived from Adipophilin (ADPH 129-137) and two control peptides: Flu MP58-66 and Melan-A27-35.
Results: The outcomes showed no significant difference between patient group and healthy donors in response to ADPH 129-137.
Conclusion: These results demonstrated that ADPH 129-137 peptide possibly does not act as an autoantigen in patients with obesity.
期刊介绍：
Acta Endocrinologica (Buc) is an international journal covering the fields of basic and clinical Endocrinology, Neuroendocrinology, Reproductive Medicine, Chronobiology, Human Ethology published quarterly
Acta Endocrinologica (Buc) is the official international journal of the Romanian Society for Endocrinology. It continues the former Romanian Journal of Endocrinology
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