William G Miller, Mary H Chapman, Tina G Williams, Delilah F Wood, James L Bono, David J Kelly
{"title":"从牛和野猪中分离出的加州弯曲杆菌新种。","authors":"William G Miller, Mary H Chapman, Tina G Williams, Delilah F Wood, James L Bono, David J Kelly","doi":"10.1099/ijsem.0.006524","DOIUrl":null,"url":null,"abstract":"<p><p>Nine <i>Campylobacter</i> strains were isolated from cattle and feral swine faeces: three were recovered during a 2007 <i>Campylobacter</i>-associated outbreak linked to a dairy, and the other six were isolated during a 2009-2010 survey of farms and ranches in Central California. The species identification of these strains could not be determined by 16S rRNA gene sequencing but were most similar to <i>Campylobacter concisus</i> and <i>Campylobacter mucosalis</i>. Additional <i>atpA</i> typing indicated that the nine strains composed a discrete novel clade related to <i>C. concisus</i> and <i>C. mucosalis</i>. A polyphasic study was undertaken here to clarify their taxonomic position. Phylogenetic analyses were performed based on 16S rRNA gene sequences and the concatenated sequences of 330 core genes. The core gene analysis placed the nine strains into a clade well separated from the other <i>Campylobacter</i> taxa, indicating that these strains represent a novel <i>Campylobacter</i> species. Pairwise digital DNA-DNA hybridization and average nucleotide identity values between these strains and other campylobacters are lower than 16 and 73%, respectively, further supporting their placement into a novel taxon. Standard phenotypic testing was performed. All strains are microaerobic or anaerobic, motile, Gram-negative, slightly-curved rods that are oxidase positive but catalase negative. Strains can be distinguished from the other catalase-negative <i>Campylobacter</i> species using phenotypic markers such as motility, oxidase activity, cephalothin resistance, hippuricase activity, growth at 30 °C, and α-haemolysis. The data presented here show that these strains represent a novel species within <i>Campylobacter</i>, for which the name <i>Campylobacter californiensis</i> sp. nov. (type strain RM6914<sup>T</sup>=LMG 32304<sup>T</sup>=CCUG 75329<sup>T</sup>) is proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 10","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457942/pdf/","citationCount":"0","resultStr":"{\"title\":\"<i>Campylobacter californiensis</i> sp. nov., isolated from cattle and feral swine.\",\"authors\":\"William G Miller, Mary H Chapman, Tina G Williams, Delilah F Wood, James L Bono, David J Kelly\",\"doi\":\"10.1099/ijsem.0.006524\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Nine <i>Campylobacter</i> strains were isolated from cattle and feral swine faeces: three were recovered during a 2007 <i>Campylobacter</i>-associated outbreak linked to a dairy, and the other six were isolated during a 2009-2010 survey of farms and ranches in Central California. The species identification of these strains could not be determined by 16S rRNA gene sequencing but were most similar to <i>Campylobacter concisus</i> and <i>Campylobacter mucosalis</i>. Additional <i>atpA</i> typing indicated that the nine strains composed a discrete novel clade related to <i>C. concisus</i> and <i>C. mucosalis</i>. A polyphasic study was undertaken here to clarify their taxonomic position. Phylogenetic analyses were performed based on 16S rRNA gene sequences and the concatenated sequences of 330 core genes. The core gene analysis placed the nine strains into a clade well separated from the other <i>Campylobacter</i> taxa, indicating that these strains represent a novel <i>Campylobacter</i> species. Pairwise digital DNA-DNA hybridization and average nucleotide identity values between these strains and other campylobacters are lower than 16 and 73%, respectively, further supporting their placement into a novel taxon. Standard phenotypic testing was performed. All strains are microaerobic or anaerobic, motile, Gram-negative, slightly-curved rods that are oxidase positive but catalase negative. Strains can be distinguished from the other catalase-negative <i>Campylobacter</i> species using phenotypic markers such as motility, oxidase activity, cephalothin resistance, hippuricase activity, growth at 30 °C, and α-haemolysis. The data presented here show that these strains represent a novel species within <i>Campylobacter</i>, for which the name <i>Campylobacter californiensis</i> sp. nov. 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Campylobacter californiensis sp. nov., isolated from cattle and feral swine.
Nine Campylobacter strains were isolated from cattle and feral swine faeces: three were recovered during a 2007 Campylobacter-associated outbreak linked to a dairy, and the other six were isolated during a 2009-2010 survey of farms and ranches in Central California. The species identification of these strains could not be determined by 16S rRNA gene sequencing but were most similar to Campylobacter concisus and Campylobacter mucosalis. Additional atpA typing indicated that the nine strains composed a discrete novel clade related to C. concisus and C. mucosalis. A polyphasic study was undertaken here to clarify their taxonomic position. Phylogenetic analyses were performed based on 16S rRNA gene sequences and the concatenated sequences of 330 core genes. The core gene analysis placed the nine strains into a clade well separated from the other Campylobacter taxa, indicating that these strains represent a novel Campylobacter species. Pairwise digital DNA-DNA hybridization and average nucleotide identity values between these strains and other campylobacters are lower than 16 and 73%, respectively, further supporting their placement into a novel taxon. Standard phenotypic testing was performed. All strains are microaerobic or anaerobic, motile, Gram-negative, slightly-curved rods that are oxidase positive but catalase negative. Strains can be distinguished from the other catalase-negative Campylobacter species using phenotypic markers such as motility, oxidase activity, cephalothin resistance, hippuricase activity, growth at 30 °C, and α-haemolysis. The data presented here show that these strains represent a novel species within Campylobacter, for which the name Campylobacter californiensis sp. nov. (type strain RM6914T=LMG 32304T=CCUG 75329T) is proposed.
期刊介绍:
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.