Fakhra Liaqat, Mahammed Ilyas Khazi, Taolin Ji, Nouman Liaqat, Yilin Le, Khalid A Al-Ghanim, Muhammad Zohaib Nawaz, Damià Barceló, Daochen Zhu
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引用次数: 0
摘要
通过生物转化将木质素转化为高价值化学品对可持续生物加工至关重要。香兰素(VN)是一种初级木质素衍生物,可被转氨为香草醛胺(VM),后者是辣椒素和药物的关键前体。这项研究建立了一种新型自给自足的氧化还原互补全细胞系统,促进了 L-丙氨酸和辅助因子的循环,从而实现了 VM 的高效生物合成。甲酸铵(AF)被用作氨基酸供体和辅助底物。与仅表达 CvTA 和 ALD 的菌株相比,共同表达 ω-反式胺酶(CvTA)、L-丙氨酸脱氢酶(ALD)和甲酸脱氢酶(FDH)的重组大肠杆菌菌株在更短的反应时间内获得了更高的产量。在优化的生物转化条件(37ºC、pH 8.0、VN:AF=1:5 和 1.5 mM NAD+)下,60 mM VN 的 VM 产率达到 97.3±1.0%。值得注意的是,构建的双质粒大肠杆菌重组体含有 CvTA、ALD、FDH 和芳香族二氧酶 (ADO),可将异丁香酚转化为 VM,产率为 73.2±1.1%。这种高效的生物转化平台不仅为辣椒素生产提供了一条可持续的VM路线,而且还促进了木质素的价值提升,从而推动了绿色生物经济的发展。
Biovalorization of Lignin-Derived Substrates to Vanillylamine via a Self-Sufficient Amino Donor and Cofactor Recycling Whole-Cell Platform.
Lignin valorization through bioconversion to high-value chemicals is crucial for sustainable bioprocessing. Vanillin (VN), a primary lignin derivative, can be transaminated into vanillylamine (VM), a key precursor for capsaicin and pharmaceuticals. This study established a novel self-sufficient redox-complementary whole-cell system, facilitating the recycling of L-alanine and cofactors for efficient VM biosynthesis. Ammonium formate (AF) was employed as amino donor and co-substrate. Recombinant E. coli strain, co-expressing ω-transaminase (CvTA), L-alanine dehydrogenase (ALD), and formate dehydrogenase (FDH), showed higher yield in shorter reaction time compared to the strain expressing only CvTA and ALD. Intermittent feeding strategy was developed to mitigate VN cytotoxicity problem and a remarkable yield of 97.3±1.0% was achieved of VM from 60 mM VN under optimized biotransamination conditions (37ºC, pH 8.0, VN:AF=1:5, and 1.5 mM NAD+). Notably, a double-plasmid E. coli recombinant harboring CvTA, ALD, FDH, and aromatic dioxygenase (ADO) was constructed to convert isoeugenol into VM with a 73.2±1.1% yield. This efficient biotransamination platform not only offers a sustainable route to VM for capsaicin production but also promotes lignin valorization for a greener bioeconomy.
期刊介绍:
The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.