设计新型偶氮苯分子光开关,通过动态调节生物膜的形成抑制细菌感染。

IF 3.8 1区 农林科学 Q1 AGRONOMY
Pest Management Science Pub Date : 2025-02-01 Epub Date: 2024-10-07 DOI:10.1002/ps.8453
Tai-Hong Zhang, Yi-Ke Yang, Yu-Mei Feng, Zhi-Jun Luo, Ming-Wei Wang, Pu-Ying Qi, Dan Zeng, Hong-Wu Liu, Yan-Mei Liao, Jiao Meng, Xiang Zhou, Li-Wei Liu, Song Yang
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引用次数: 0

摘要

背景:细菌生物膜是细菌抵御外界恶劣环境的坚强堡垒,可增强细菌的耐受性,加剧耐药性/耐药性风险。目前,光药理学提供了一种先进的方法,通过精确的时空控制,以光控制分子构型来调节生物活性,从而在药物/杀虫剂的开发方面具有巨大潜力:为了进一步拓展光药理学在发现新型抗生物膜剂方面的应用,我们制备了一系列光控偶氮活性分子,并对其光异构化、抗疲劳性和抗生物膜性能进行了探索。此外,我们还系统地研究了它们抑制生物膜形成的机理。总体而言,所设计的偶氮衍生物 A11 具有优异的抗 Xoo 活性,其半最大有效浓度(EC50)值为 5.45 μg mL-1,在紫外线照射下(转换为顺式构型),EC50 值可进一步升高至 2.19 μg mL-1。光开关行为表明 A11 具有出色的抗疲劳特性。对其作用机制的深入分析表明,A11 能有效抑制生物膜的形成和相关毒力因子的表达。这种性能可以通过加载私人光开关特性进行动态调节:在这项工作中,设计的光控偶氮分子为通过动态调节细菌生物膜的形成来抵抗细菌感染提供了一种新的模式。© 2024 化学工业协会。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Engineering the novel azobenzene-based molecular photoswitches for suppressing bacterial infection through dynamic regulation of biofilm formation.

Engineering the novel azobenzene-based molecular photoswitches for suppressing bacterial infection through dynamic regulation of biofilm formation.

Background: Bacterial biofilm is a strong fortress for bacteria to resist harsh external environments, which can enhance their tolerance and exacerbate the drug/pesticide resistance risk. Currently, photopharmacology provides an advanced approach via precise spatiotemporal control for regulating biological activities by light-controlling the molecular configurations, thereby having enormous potential in the development of drug/pesticides.

Results: To further expand the photopharmacology application for discovering new antibiofilm agents, we prepared a series of light-controlled azo-active molecules and explored their photo isomerization, fatigue resistance, and anti-biofilm performance. Furthermore, their mechanisms of inhibiting biofilm formation were systematically investigated. Overall, designed azo-derivative A11 featured excellent anti-Xoo activity with an half-maximal effective concentration (EC50) value of 5.45 μg mL-1, and the EC50 value could be further elevated to 2.19 μg mL-1 after ultraviolet irradiation (converted as cis-configuration). The photo-switching behavior showed that A11 had outstanding anti-fatigue properties. An in-depth analysis of the action mechanism showed that A11 could effectively inhibit biofilm formation and the expression of relevant virulence factors. This performance could be dynamically regulated via loading with private light-switch property.

Conclusion: In this work, designed light-controlled azo molecules provide a new model for resisting bacterial infection via dynamic regulation of bacterial biofilm formation. © 2024 Society of Chemical Industry.

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来源期刊
Pest Management Science
Pest Management Science 农林科学-昆虫学
CiteScore
7.90
自引率
9.80%
发文量
553
审稿时长
4.8 months
期刊介绍: Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.
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