三维光片荧光显微镜在临床前和临床药物发现中的应用。

IF 6.5 2区 医学 Q1 PHARMACOLOGY & PHARMACY
Urmas Roostalu, Henrik H. Hansen, Jacob Hecksher-Sørensen
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引用次数: 0

摘要

光片荧光显微镜(LSFM)与组织清除相结合,已成为药物发现领域的一项强大技术。LSFM 适用于从啮齿动物器官到临床组织活检的各种样本,已被用于鉴定组织中的药物靶点,展示药物的生物分布,并确定其疗效和作用模式。LSFM 可扩展到高通量分析,并提供低至单细胞水平的分辨率。在本综述中,我们将介绍将 LSFM 应用于药物发现管道的优势,并重点介绍该领域的最新进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
3D light-sheet fluorescence microscopy in preclinical and clinical drug discovery
Light-sheet fluorescence microscopy (LSFM) combined with tissue clearing has emerged as a powerful technology in drug discovery. LSFM is applicable to a variety of samples, from rodent organs to clinical tissue biopsies, and has been used for characterizing drug targets in tissues, demonstrating the biodistribution of pharmaceuticals and determining their efficacy and mode of action. LSFM is scalable to high-throughput analysis and provides resolution down to the single cell level. In this review, we describe the advantages of implementing LSFM into the drug discovery pipeline and highlight recent advances in this field.
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来源期刊
Drug Discovery Today
Drug Discovery Today 医学-药学
CiteScore
14.80
自引率
2.70%
发文量
293
审稿时长
6 months
期刊介绍: Drug Discovery Today delivers informed and highly current reviews for the discovery community. The magazine addresses not only the rapid scientific developments in drug discovery associated technologies but also the management, commercial and regulatory issues that increasingly play a part in how R&D is planned, structured and executed. Features include comment by international experts, news and analysis of important developments, reviews of key scientific and strategic issues, overviews of recent progress in specific therapeutic areas and conference reports.
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