元基因组新一代测序技术在重症监护病房免疫功能低下的严重呼吸道感染患者中的临床应用。

IF 5.8 2区 医学 Q1 Medicine
Junjie Zhao, Yong Sun, Jing Tang, Kai Guo, Kaiyu Wang, Jiancheng Zhuge, Honglong Fang
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引用次数: 0

摘要

背景:早期靶向抗生素治疗对于改善重症监护室(ICU)中患有严重呼吸道感染(SRI)的免疫功能低下患者的预后至关重要。元基因组下一代测序(mNGS)在病原体检测方面具有重要价值,但对下呼吸道微生物的研究仍然有限:本研究招募了 234 名重症监护室的 SRI 患者,将其分为免疫功能低下组和免疫功能健全组。我们比较了使用支气管肺泡灌洗液(BALF)的 mNGS 与传统微生物检验(CMT)的诊断性能,并分析了 mNGS 在 ICU 中免疫功能低下的 SRI 患者中的价值:在所有患者中,mNGS 的病原微生物检出率高于 CMT(94.02% vs 66.67%,P 0.05;42.67% vs 45.83%,P > 0.05)。对于经验性治疗效果不佳的免疫功能低下患者,mNGS 可以提供病原学依据,帮助更精确地调整抗生素方案,从而改善患者的预后。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The clinical application of metagenomic next-generation sequencing in immunocompromised patients with severe respiratory infections in the ICU.

Background: Early targeted antibiotic therapy is crucial for improving the prognosis of immunocompromised patients with severe respiratory infections (SRIs) in the intensive care unit (ICU). Metagenomic next-generation sequencing (mNGS) has shown significant value in pathogen detection, but research on lower respiratory tract microorganisms remains limited.

Methods: This study enrolled 234 patients with SRIs in the ICU, and individuals were categorized into immunocompromised and immunocompetent groups. We compared the diagnostic performance of mNGS using bronchoalveolar lavage fluid (BALF) with conventional microbiological tests (CMTs) and analyzed the value of mNGS in immunocompromised patients with SRIs in the ICU.

Results: Among all patients, the pathogenic microorganism detection rate of mNGS was higher than that of CMTs (94.02% vs 66.67%, P < 0.05), both in the immunocompromised group (95.0% vs 58.75%, P < 0.05) and the immunocompetent group (93.51% vs 71.43%, P < 0.05). mNGS detected more pathogens than CMTs did (167 vs 51), identifying 116 organisms that were missed by CMTs. The proportion of antibiotic regimen adjustments based on mNGS results was significantly higher compared to CMTs in both the immunocompromised (70.00% vs 17.50%, P < 0.05) and immunocompetent groups (48.70% vs 15.58%, P < 0.05). In the immunocompromised group, patients who had their antibiotic treatment adjusted on mNGS results had improved prognosis, with significantly lower ICU mortality (8.93% vs 50%, P < 0.05) and 28-day mortality rates (30.36% vs 68.75%, P < 0.05) than CMTs. In the immunocompetent group, no statistically significant differences were observed in ICU mortality or 28-day mortality (20.00% vs 33.33%, P > 0.05; 42.67% vs 45.83%, P > 0.05).

Conclusion: mNGS shows significant value in detecting pathogens in immunocompromised patients with SRIs in ICU. For immunocompromised patients who respond poorly to empirical treatment, mNGS can provide an etiological basis, helping adjust antibiotic regimens more precisely and thereby improving patient prognosis.

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来源期刊
Respiratory Research
Respiratory Research RESPIRATORY SYSTEM-
CiteScore
9.70
自引率
1.70%
发文量
314
审稿时长
4-8 weeks
期刊介绍: Respiratory Research publishes high-quality clinical and basic research, review and commentary articles on all aspects of respiratory medicine and related diseases. As the leading fully open access journal in the field, Respiratory Research provides an essential resource for pulmonologists, allergists, immunologists and other physicians, researchers, healthcare workers and medical students with worldwide dissemination of articles resulting in high visibility and generating international discussion. Topics of specific interest include asthma, chronic obstructive pulmonary disease, cystic fibrosis, genetics, infectious diseases, interstitial lung diseases, lung development, lung tumors, occupational and environmental factors, pulmonary circulation, pulmonary pharmacology and therapeutics, respiratory immunology, respiratory physiology, and sleep-related respiratory problems.
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