Manar A. Ahmad, Nashaat Nabil Ismail, Sherien S. Ghaleb, Dalia Abd Elwhab Hassan, Reham S. Al-Fakharany, Mohammed M. Ghoneim, Amany M. Ahmed, Amr Mohamed Lotfy, Ahmed Fathy Mohamed AboSief
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Full history, local examination, semen analysis, and urine and blood samples were taken from all participants. Urinary Bisphenol A, malondialdehyde, and serum microRNA-337 were measured.</p><h3>Results</h3><p>The mean Bisphenol A level in azoospermia group shows statistically significant increase comparing to fertile control group. The mean microRNA-337 level in oligozoospermia and azoospermia group shows statistically significant increase comparing to fertile controls. The mean malondialdehyde level in infertility groups shows statistically significant increase comparing to fertile control group. No linear correlations were recorded between Bisphenol A levels with semen quality parameters, hormonal profile, and microRNA-337.</p><h3>Conclusion</h3><p>While there is no significant change in the levels of Bisphenol A between normal fertile males and infertile males with oligozospermia, a significant elevation in the BPA level was observed in infertile males with azoospermia. A significant upregulation of the miRNA-337 gene expression in infertile males either oligozospermia or azoospermia was also observed. In addition, lipid peroxidation as evident by the significant elevation of MDA levels was marked among infertile patients.</p></div>","PeriodicalId":481,"journal":{"name":"Beni-Suef University Journal of Basic and Applied Sciences","volume":"13 1","pages":""},"PeriodicalIF":2.5000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://bjbas.springeropen.com/counter/pdf/10.1186/s43088-024-00559-w","citationCount":"0","resultStr":"{\"title\":\"High levels of bisphenol A among infertile men can impair spermatogenesis by oxidative stress and elevated levels of microRNA-337\",\"authors\":\"Manar A. Ahmad, Nashaat Nabil Ismail, Sherien S. Ghaleb, Dalia Abd Elwhab Hassan, Reham S. Al-Fakharany, Mohammed M. Ghoneim, Amany M. 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A significant upregulation of the miRNA-337 gene expression in infertile males either oligozospermia or azoospermia was also observed. 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引用次数: 0
摘要
背景研究表明，双酚 A 可能会干扰精子生成过程，导致精液质量下降。然而，其基本过程仍不清楚。本研究旨在调查不育男性暴露于双酚 A、精子发生与 microRNA-337 和丙二醛之间的联系。不育组（1a）：无精子症（16 人）；不育组（1b）：少精子症（22 人）；对照组（2）：正常精子症（35 人）。研究人员对所有参与者进行了病史采集、局部检查、精液分析、尿液和血液样本采集。结果与可育对照组相比，无精子症组的平均双酚 A 水平有统计学意义的显著增加。与可育对照组相比，少精症和无精症组的平均 microRNA-337 水平有统计学意义的显著增加。与可育对照组相比，不育组的丙二醛平均水平在统计学上有明显增加。结论虽然正常可育男性和少精子症不育男性的双酚 A 水平没有明显变化，但在无精子症不育男性中观察到双酚 A 水平明显升高。在少精症或无精症的不育男性中，还观察到 miRNA-337 基因表达明显上调。此外，不育症患者的 MDA 水平明显升高，表明脂质过氧化反应明显。
High levels of bisphenol A among infertile men can impair spermatogenesis by oxidative stress and elevated levels of microRNA-337
Background
Studies have shown that Bisphenol A may interfere with the process of spermatogenesis and result in a decrease in the quality of semen. Nevertheless, the fundamental processes remain unclear. This study was done to investigate the connections between exposure to Bisphenol A, spermatogenesis with microRNA-337, and malondialdehyde in infertile men.
Methods
This study was a case–control study on 73 participants. Infertile group (1a): azoospermia (n = 16), infertile group (1b): oligozoospermia (n = 22), and control group (2): normospermic (n = 35) were enrolled in this study. Full history, local examination, semen analysis, and urine and blood samples were taken from all participants. Urinary Bisphenol A, malondialdehyde, and serum microRNA-337 were measured.
Results
The mean Bisphenol A level in azoospermia group shows statistically significant increase comparing to fertile control group. The mean microRNA-337 level in oligozoospermia and azoospermia group shows statistically significant increase comparing to fertile controls. The mean malondialdehyde level in infertility groups shows statistically significant increase comparing to fertile control group. No linear correlations were recorded between Bisphenol A levels with semen quality parameters, hormonal profile, and microRNA-337.
Conclusion
While there is no significant change in the levels of Bisphenol A between normal fertile males and infertile males with oligozospermia, a significant elevation in the BPA level was observed in infertile males with azoospermia. A significant upregulation of the miRNA-337 gene expression in infertile males either oligozospermia or azoospermia was also observed. In addition, lipid peroxidation as evident by the significant elevation of MDA levels was marked among infertile patients.
期刊介绍：
Beni-Suef University Journal of Basic and Applied Sciences (BJBAS) is a peer-reviewed, open-access journal. This journal welcomes submissions of original research, literature reviews, and editorials in its respected fields of fundamental science, applied science (with a particular focus on the fields of applied nanotechnology and biotechnology), medical sciences, pharmaceutical sciences, and engineering. The multidisciplinary aspects of the journal encourage global collaboration between researchers in multiple fields and provide cross-disciplinary dissemination of findings.
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