通过对完整三叉神经节神经元进行活体 GCaMP Ca2+ 成像,阐明颞下颌关节损伤和炎症小鼠模型中的神经元活动。

IF 5.9 1区 医学 Q1 ANESTHESIOLOGY
PAIN® Pub Date : 2024-12-01 Epub Date: 2024-10-01 DOI:10.1097/j.pain.0000000000003421
Hyeonwi Son, John Shannonhouse, Yan Zhang, Ruben Gomez, Felix Amarista, Daniel Perez, Edward Ellis, Man-Kyo Chung, Yu Shin Kim
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引用次数: 0

摘要

摘要:颞下颌关节紊乱症(TMD)患者通常会感到面部疼痛、颞下颌关节(TMJ)不适或压痛,从而导致日常生活障碍。遗憾的是,现有的 TMD 治疗方法并不总是有效,因此需要更先进的、基于机制的疗法。在这项研究中,我们使用完整三叉神经节(TG)的体内 GCaMP3 Ca2+ 成像来描述体内三叉神经节神经元的功能活动,特别是在颞下颌关节损伤和炎症的小鼠模型中。该系统使我们能够在完整的解剖、生理和临床条件下观察神经元活动,并评估神经元功能和对各种刺激的反应。我们观察到,在注射完全弗氏佐剂或强迫张口(FMO)的小鼠颞下颌关节中,自发和瞬时激活的神经元对机械、热和化学刺激的反应明显增加。降钙素基因相关肽受体抑制剂能显著减轻 FMO 引起的面部超敏反应。此外,我们还通过对完整 TG 的体内 GCaMP3 Ca2+ 成像证实了降钙素基因相关肽拮抗剂对 FMO 诱导的过敏性的减弱作用。我们的研究结果有助于揭示颞下颌关节神经元在颞下颌关节疼痛中的作用和活动,使我们更接近于理解颞下颌关节损伤后颞下颌关节疼痛的病理生理过程。我们的研究还说明了体内 GCaMP3 Ca2+ 成像对完整颞下颌关节疼痛的实用性,其研究目的是开发更有针对性和更有效的颞下颌关节疼痛治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Elucidation of neuronal activity in mouse models of temporomandibular joint injury and inflammation by in vivo GCaMP Ca 2+ imaging of intact trigeminal ganglion neurons.

Abstract: Patients with temporomandibular disorders (TMDs) typically experience facial pain and discomfort or tenderness in the temporomandibular joint (TMJ), causing disability in daily life. Unfortunately, existing treatments for TMD are not always effective, creating a need for more advanced, mechanism-based therapies. In this study, we used in vivo GCaMP3 Ca 2+ imaging of intact trigeminal ganglia (TG) to characterize functional activity of the TG neurons in vivo, specifically in mouse models of TMJ injury and inflammation. This system allows us to observe neuronal activity in intact anatomical, physiological, and clinical conditions and to assess neuronal function and response to various stimuli. We observed a significant increase in spontaneously and transiently activated neurons responding to mechanical, thermal, and chemical stimuli in the TG of mice with TMJ injection of complete Freund adjuvant or with forced mouth opening (FMO). An inhibitor of the calcitonin gene-related peptide receptor significantly attenuated FMO-induced facial hypersensitivity. In addition, we confirmed the attenuating effect of calcitonin gene-related peptide antagonist on FMO-induced sensitization by in vivo GCaMP3 Ca 2+ imaging of intact TG. Our results contribute to unraveling the role and activity of TG neurons in the TMJ pain, bringing us closer to understanding the pathophysiological processes underlying TMJ pain after TMJ injury. Our study also illustrates the utility of in vivo GCaMP3 Ca 2+ imaging of intact TG for studies aimed at developing more targeted and effective treatments for TMJ pain.

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来源期刊
PAIN®
PAIN® 医学-临床神经学
CiteScore
12.50
自引率
8.10%
发文量
242
审稿时长
9 months
期刊介绍: PAIN® is the official publication of the International Association for the Study of Pain and publishes original research on the nature,mechanisms and treatment of pain.PAIN® provides a forum for the dissemination of research in the basic and clinical sciences of multidisciplinary interest.
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