Bo-Youn Moon, Md Sekendar Ali, Seunghoe Kim, Hee-Seung Kang, Ye-Ji Kang, Jae-Myung Kim, Dong-Chan Moon, Suk-Kyung Lim
{"title":"从韩国狗体内分离的耐碳青霉烯类大肠埃希菌的流行率和分子特征。","authors":"Bo-Youn Moon, Md Sekendar Ali, Seunghoe Kim, Hee-Seung Kang, Ye-Ji Kang, Jae-Myung Kim, Dong-Chan Moon, Suk-Kyung Lim","doi":"10.4142/jvs.24164","DOIUrl":null,"url":null,"abstract":"<p><strong>Importance: </strong>Carbapenem-resistant <i>Enterobacteriaceae</i> are emerging as a global public health risk. Therefore, assessing the prevalence of carbapenem-resistant <i>Escherichia coli</i> (CRE) in both humans and animals is important.</p><p><strong>Objective: </strong>We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats.</p><p><strong>Methods: </strong><i>E. coli</i> strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE).</p><p><strong>Results: </strong>In total, 13 CRE isolates (0.13%) were identified from dogs possessing <i>bla</i><sub>NDM-5</sub> along with β-lactamase genes, mostly <i>bla</i><sub>CMY-2</sub> (92.2%) and <i>bla</i><sub>TEM-1</sub> (53.8%). The commonly observed mutations were S83L and D87N in <i>gyrA</i>, S80I in <i>parC</i>, and S458A in <i>parE</i>. CRE carried non-beta-lactam resistance genes, with the majority being <i>tet</i>(B) (100%), <i>sul</i> (84.6%), and <i>aac(3)-II</i> (53.8%). Nine different PFGE patterns (P1-P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected.</p><p><strong>Conclusions and relevance: </strong>This investigation provides significant insight into the prevalence and molecular characteristics of <i>bla</i><sub>NDM-5</sub>-carrying <i>E. coli</i> in dogs. The co-existence of <i>bla</i><sub>NDM-5</sub> and other antimicrobial resistance genes in <i>E. coli</i> potentially poses severe health hazards to humans.</p>","PeriodicalId":17557,"journal":{"name":"Journal of Veterinary Science","volume":"25 5","pages":"e67"},"PeriodicalIF":1.5000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450394/pdf/","citationCount":"0","resultStr":"{\"title\":\"Prevalence and molecular characteristics of carbapenem-resistant <i>Escherichia coli</i> isolated from dogs in South Korea.\",\"authors\":\"Bo-Youn Moon, Md Sekendar Ali, Seunghoe Kim, Hee-Seung Kang, Ye-Ji Kang, Jae-Myung Kim, Dong-Chan Moon, Suk-Kyung Lim\",\"doi\":\"10.4142/jvs.24164\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Importance: </strong>Carbapenem-resistant <i>Enterobacteriaceae</i> are emerging as a global public health risk. Therefore, assessing the prevalence of carbapenem-resistant <i>Escherichia coli</i> (CRE) in both humans and animals is important.</p><p><strong>Objective: </strong>We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats.</p><p><strong>Methods: </strong><i>E. coli</i> strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE).</p><p><strong>Results: </strong>In total, 13 CRE isolates (0.13%) were identified from dogs possessing <i>bla</i><sub>NDM-5</sub> along with β-lactamase genes, mostly <i>bla</i><sub>CMY-2</sub> (92.2%) and <i>bla</i><sub>TEM-1</sub> (53.8%). The commonly observed mutations were S83L and D87N in <i>gyrA</i>, S80I in <i>parC</i>, and S458A in <i>parE</i>. CRE carried non-beta-lactam resistance genes, with the majority being <i>tet</i>(B) (100%), <i>sul</i> (84.6%), and <i>aac(3)-II</i> (53.8%). Nine different PFGE patterns (P1-P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected.</p><p><strong>Conclusions and relevance: </strong>This investigation provides significant insight into the prevalence and molecular characteristics of <i>bla</i><sub>NDM-5</sub>-carrying <i>E. coli</i> in dogs. The co-existence of <i>bla</i><sub>NDM-5</sub> and other antimicrobial resistance genes in <i>E. coli</i> potentially poses severe health hazards to humans.</p>\",\"PeriodicalId\":17557,\"journal\":{\"name\":\"Journal of Veterinary Science\",\"volume\":\"25 5\",\"pages\":\"e67\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450394/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.4142/jvs.24164\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.4142/jvs.24164","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Prevalence and molecular characteristics of carbapenem-resistant Escherichia coli isolated from dogs in South Korea.
Importance: Carbapenem-resistant Enterobacteriaceae are emerging as a global public health risk. Therefore, assessing the prevalence of carbapenem-resistant Escherichia coli (CRE) in both humans and animals is important.
Objective: We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats.
Methods: E. coli strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE).
Results: In total, 13 CRE isolates (0.13%) were identified from dogs possessing blaNDM-5 along with β-lactamase genes, mostly blaCMY-2 (92.2%) and blaTEM-1 (53.8%). The commonly observed mutations were S83L and D87N in gyrA, S80I in parC, and S458A in parE. CRE carried non-beta-lactam resistance genes, with the majority being tet(B) (100%), sul (84.6%), and aac(3)-II (53.8%). Nine different PFGE patterns (P1-P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected.
Conclusions and relevance: This investigation provides significant insight into the prevalence and molecular characteristics of blaNDM-5-carrying E. coli in dogs. The co-existence of blaNDM-5 and other antimicrobial resistance genes in E. coli potentially poses severe health hazards to humans.
期刊介绍：
The Journal of Veterinary Science (J Vet Sci) is devoted to the advancement and dissemination of scientific knowledge concerning veterinary sciences and related academic disciplines. It is an international journal indexed in the Thomson Scientific Web of Science, SCI-EXPANDED, Sci Search, BIOSIS Previews, Biological Abstracts, Focus on: Veterinary Science & Medicine, Zoological Record, PubMed /MEDLINE, Index Medicus, Pubmed Central, CAB Abstracts / Index Veterinarius, EBSCO, AGRIS and AGRICOLA. This journal published in English by the Korean Society of Veterinary Science (KSVS) being distributed worldwide.
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