利用实验设计,开发一种通过固相微萃取箭头空间-气相色谱-质谱法分析洋葱挥发性成分的非目标方法。

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Lea Böckstiegel, Theresa Schaumann, Björn Egert, Sabine E. Kulling, Christoph H. Weinert
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引用次数: 0

摘要

洋葱的独特香气主要由含硫化合物组成,这也是洋葱广受欢迎的原因之一。洋葱球茎组织被破坏后会迅速发生酶促和化学反应,这给挥发性成分的分析带来了挑战。因此,样品制备是分析洋葱挥发物的关键步骤,但在文献中往往没有得到必要的重视。在这项工作中,我们重点关注两个方面:首先,我们比较了不同的样品制备方法,以最大限度地提高挥发物的产量,并描述固相微萃取(SPME)箭式取样程序后出现的洋葱挥发物特征。就顶空(HS)-气相色谱-质谱分析而言,加入硫酸铵溶液的洋葱汁被证明是最佳选择。其次,我们利用高效的实验设计(DoE)方法优化了洋葱挥发物的顶空取样和解吸参数(富集时间、富集温度、搅拌器速度、解吸时间)。使用 HS-SPME Arrow 分析的最佳条件是:富集时间为 75 分钟,温度为 60°C,搅拌器转速为 713 rpm,解吸时间为 120 秒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of an Untargeted Method for the Analysis of the Volatile Profile of Onions via Solid-Phase Microextraction Arrow-Headspace-Gas Chromatography-Mass Spectrometry Using Design of Experiments

The distinctive aroma of onions, consisting primarily of sulfur-containing compounds, is one of the reasons for the popularity of the vegetable. The rapid enzymatic and chemical reactions that occur after the destruction of onion bulb tissue render the analysis of the volatile profile challenging. Therefore, sample preparation is a crucial step in the analysis of the onion volatilome, but it often does not receive the necessary attention in the literature. In this work, we focused on two aspects: Firstly, we compared different sample preparation approaches to maximize the volatile yield and to enable the description of the onion volatile profile as it would emerge after a solid-phase microextraction (SPME) Arrow sampling procedure. For headspace (HS)-gas chromatography-mass spectrometry analysis, onion juice with the addition of an ammonium sulfate solution proved to be the best option. Secondly, we optimized the HS sampling and desorption parameters (enrichment time, enrichment temperature, agitator speed, desorption time) for onion volatiles using the efficient design of experiments (DoE) approach. The optimal conditions for the analysis with HS-SPME Arrow were an enrichment time of 75 min at 60°C with an agitator speed of 713 rpm and a desorption time of 120 s.

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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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