{"title":"猪马葡萄球菌中携带多重抗性基因 cfr 的两种新型质粒。","authors":"","doi":"10.1016/j.jgar.2024.09.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>The emergence and transmission of the multidrug resistance gene <em>cfr</em> have raised public health concerns worldwide.</div></div><div><h3>Objectives</h3><div>Multidrug-resistant <em>Staphylococcus equorum</em> isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one <em>Staphylococcus equorum</em> isolate harbouring two distinct <em>cfr</em>-carrying plasmids.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. <em>De novo</em> hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools.</div></div><div><h3>Results</h3><div><em>Staphylococcus equorum</em> isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two <em>cfr</em>-harbouring plasmids: the RepA N-family plasmid pSN42–51 K and the Inc18-family plasmid pSN42–50 K. These two plasmids exhibited low structural similarities to the so far reported <em>cfr</em>-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene <em>lsa</em>(B). In addition, plasmid pSN42–51 K carried two <em>erm</em>(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene <em>ant(6)-Ia</em> as well as mercury resistance genes while pSN42–50 K was associated with the heavy metal translocating P-type ATPase gene <em>hmtp</em>. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the <em>cfr</em>-carrying plasmids.</div></div><div><h3>Conclusion</h3><div>This is the first report of <em>S. equorum</em> carrying two distinct <em>cfr</em>-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health.</div></div>","PeriodicalId":15936,"journal":{"name":"Journal of global antimicrobial resistance","volume":null,"pages":null},"PeriodicalIF":3.7000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum\",\"authors\":\"\",\"doi\":\"10.1016/j.jgar.2024.09.004\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><div>The emergence and transmission of the multidrug resistance gene <em>cfr</em> have raised public health concerns worldwide.</div></div><div><h3>Objectives</h3><div>Multidrug-resistant <em>Staphylococcus equorum</em> isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one <em>Staphylococcus equorum</em> isolate harbouring two distinct <em>cfr</em>-carrying plasmids.</div></div><div><h3>Methods</h3><div>Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. <em>De novo</em> hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools.</div></div><div><h3>Results</h3><div><em>Staphylococcus equorum</em> isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two <em>cfr</em>-harbouring plasmids: the RepA N-family plasmid pSN42–51 K and the Inc18-family plasmid pSN42–50 K. These two plasmids exhibited low structural similarities to the so far reported <em>cfr</em>-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene <em>lsa</em>(B). In addition, plasmid pSN42–51 K carried two <em>erm</em>(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene <em>ant(6)-Ia</em> as well as mercury resistance genes while pSN42–50 K was associated with the heavy metal translocating P-type ATPase gene <em>hmtp</em>. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the <em>cfr</em>-carrying plasmids.</div></div><div><h3>Conclusion</h3><div>This is the first report of <em>S. equorum</em> carrying two distinct <em>cfr</em>-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health.</div></div>\",\"PeriodicalId\":15936,\"journal\":{\"name\":\"Journal of global antimicrobial resistance\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of global antimicrobial resistance\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213716524001723\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of global antimicrobial resistance","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213716524001723","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
引用次数: 0
摘要
背景耐多药基因 cfr 的出现和传播引起了全球公共卫生的关注:耐多药的马葡萄球菌可对公共卫生构成威胁。在这项研究中,我们对一个携带两种不同的 cfr 质粒的马葡萄球菌分离株的全基因组进行了鉴定:方法:采用肉汤微稀释法进行抗菌药敏感性测试。使用 Illumina HiSeq X Ten 和 Nanopore MinION 平台对基因组 DNA 进行测序。由 Unicycler 进行从头杂交组装。基因组数据通过硅预测和生物信息学工具进行评估:马葡萄球菌分离株 SN42 对利奈唑胺、红霉素、四环素、氧西林、克林霉素、维吉尼霉素、替米考林、氯霉素和氟苯尼考具有耐药性或高 MICs。它携带了两个携带 cfr 的质粒:RepA N-家族质粒 pSN42-51K 和 Inc18-家族质粒 pSN42-50K。这两个质粒在结构上与迄今报道的携带 cfr 的质粒相似度较低。这两个质粒都含有抗砷操作子、抗铜和镉基因以及抗林可霉素-缬氨嘧啶-链霉毒素 A 基因 lsa(B)。此外,质粒 pSN42-51K 还携带了两个抗大环内酯-林可酰胺-链霉素 B 的 erm(B) 基因、链霉素抗性基因 ant(6)-Ia 以及汞抗性基因,而 pSN42-50K 则与重金属转运 P 型 ATP 酶基因 hmtp 有关。这些抗菌素抗性基因和重金属抗性基因的共同携带和共存增加了携带 cfr 的质粒共同选择的可能性:这是首次报告马铃薯沙门氏菌(S. equorum)携带两种不同的cfr-携带质粒,这突出表明有必要进行持续监测,以应对产自食用动物的细菌可能传播的多重耐药性,从而确保食品安全和公众健康。
Two novel plasmids harbouring the multiresistance gene cfr in porcine Staphylococcus equorum
Background
The emergence and transmission of the multidrug resistance gene cfr have raised public health concerns worldwide.
Objectives
Multidrug-resistant Staphylococcus equorum isolates can pose a threat to public health. In this study, we have characterised the whole-genome of one Staphylococcus equorum isolate harbouring two distinct cfr-carrying plasmids.
Methods
Antimicrobial susceptibility testing was performed by broth microdilution. Genomic DNA was sequenced using both the Illumina HiSeq X Ten and Nanopore MinION platforms. De novo hybrid assembly was performed by Unicycler. Genomic data were assessed by in silico prediction and bioinformatic tools.
Results
Staphylococcus equorum isolate SN42 exhibited resistance or high MICs to linezolid, erythromycin, tetracycline, oxacillin, clindamycin, virginiamycin, tiamulin, chloramphenicol and florfenicol. It carried two cfr-harbouring plasmids: the RepA N-family plasmid pSN42–51 K and the Inc18-family plasmid pSN42–50 K. These two plasmids exhibited low structural similarities to the so far reported cfr-carrying plasmids. Both plasmids harboured an arsenic resistance operon, copper and cadmium resistance genes as well as the lincosamide-pleuromutilin-streptogramin A resistance gene lsa(B). In addition, plasmid pSN42–51 K carried two erm(B) genes for macrolide-lincosamide-streptogramin B resistance, the streptomycin resistance gene ant(6)-Ia as well as mercury resistance genes while pSN42–50 K was associated with the heavy metal translocating P-type ATPase gene hmtp. The co-carriage and co-existence of these antimicrobial resistance and heavy metal resistance genes increases the likelihood of co-selection of the cfr-carrying plasmids.
Conclusion
This is the first report of S. equorum carrying two distinct cfr-carrying plasmids, underscoring the need for ongoing surveillance to address the potential dissemination of multi-drug resistance in bacteria from food-producing animals to ensure food safety and public health.
期刊介绍:
The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes.
JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR).
Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.