青蒿中的胚芽烷类倍半萜及其抗炎活性。

IF 2.5 3区医学 Q3 CHEMISTRY, MEDICINAL

Fitoterapia Pub Date : 2024-10-01 DOI:10.1016/j.fitote.2024.106241

Chunping Tang , Yongzhe Zheng , Zhengguang Shao , Chang-Qiang Ke , Zheling Feng , Yang Ye

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引用次数: 0

摘要

蒿属植物以其丰富的倍半萜化合物而闻名，这些化合物具有多种结构类型，并表现出一系列生物活性。在这项研究中，对青蒿的系统研究首次发现了胚芽蒿类倍半萜化合物。研究发现了 10 种新化合物和 3 种已知类似物，其中包括两种罕见的二聚胚芽萜类化合物。通过涉及质谱、红外光谱、一维和二维核磁共振光谱数据、单晶 X 射线衍射、密度泛函理论（DFT）核磁共振计算和时间相关 DFT 电子圆二色性（TDDFT ECD）计算的综合分析，对这些化合物的结构进行了全面鉴定。此外，还评估了所有分离化合物在 LPS 刺激的 RAW 264.7 鼠巨噬细胞中的抗炎活性。化合物 10 对 NO 的产生具有强效抑制作用，其 IC50 值为 4.01 ± 0.09 μM。这项研究凸显了青蒿物种多样的化学成分，并强调了它们在药物发现和开发方面的潜力。

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Germacrane-type sesquiterpenes from Artemisia atrovirens and their anti-inflammatory activity

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Germacrane-type sesquiterpenes from Artemisia atrovirens and their anti-inflammatory activity

Artemisia plants are well-known for their abundant sesquiterpene compounds, which encompass various structural types and exhibit a range of biological activities. In this study, a systematic investigation of Artemisia atrovirens revealed the presence of germacrane-type sesquiterpenes for the first time. This included the discovery of 10 new compounds and three known analogues, among which were two rare dimeric germacrane-type compounds. Their structures were fully characterized through a comprehensive analysis involving MS, IR, 1D- and 2D-NMR spectroscopic data, single crystal X-ray diffraction, density functional theory (DFT) NMR calculations, and time-dependent DFT electronic circular dichroism (TDDFT ECD) calculations. Furthermore, all isolated compounds were evaluated for their anti-inflammatory activity in LPS-stimulated RAW 264.7 murine macrophages. Compound 10 demonstrated a potent inhibitory effect on NO production, with an IC₅₀ value of 4.01 ± 0.09 μM. This study highlights the diverse chemical repertoire of Artemisia species and underscores their potential in drug discovery and development.

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来源期刊

Fitoterapia 医学-药学

CiteScore

5.80

自引率

2.90%

发文量

198

审稿时长

1.5 months

期刊介绍： Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.