通过 qPCR 检测墨西哥灵长类动物中的丝虫感染。

IF 2 3区 生物学 Q1 ZOOLOGY
Brenda Solórzano-García, Norberto Colín García, Filippo Aureli, Gerardo Pérez-Ponce de León
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引用次数: 0

摘要

丝虫是一种寄生线虫,在兽医和医学方面具有重要意义,是一些急性热带疾病的罪魁祸首。它们通过咬蠓和黑蝇等食血媒介的叮咬传播。丝虫是新热带灵长类动物中最常见的病媒寄生虫之一,严重感染可导致炎症反应和组织损伤。由于丝虫寄生在宿主体内(腹腔、血液和淋巴管),丝虫线虫的检测具有挑战性,而且大多是在死后进行的,因此有关丝虫在野生灵长类种群中流行情况的研究很少。在此,我们结合非侵入性采样和分子诊断技术,报告了丝虫感染在墨西哥南部自由活动的杰弗里蜘蛛猴(Ateles geoffroyi)和黑吼猴(Alouatta pigra)种群中的流行情况。通过 qPCR 协议对粪便样本进行丝虫 DNA 筛查。共检测了 88 份样本,总体流行率为 26%。与吼猴相比,丝虫在蜘蛛猴中更为常见。这项研究首次报告了丝虫线虫在墨西哥南部野生蜘蛛猴群体中的感染率,也首次报告了丝虫在黑吼猴中的感染率,这是灵长类寄生虫学和寄生虫感染诊断新时代的一部分,因为现在的分子工具更加经济实惠。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Detection of Filariid Infections in Mexican Primate Populations Through qPCR

Detection of Filariid Infections in Mexican Primate Populations Through qPCR

Filariae are parasitic nematodes of high veterinary and medical importance, responsible for some acute tropical diseases. They are transmitted through the bite of hematophagous vectors such as biting midges and blackflies. Filariae are among the most prevalent vector-borne parasitoses in Neotropical primates in which severe infections can cause inflammatory reactions and tissue damage. Given the location inside the host (peritoneal cavity, bloodstream, and lymphatics), the detection of filariid nematodes is challenging and is mostly postmortem; hence the scarcity of studies on the prevalence of filariae in wild primate populations. Here, we report the prevalence of filariid infections in free-ranging populations of Geoffroy's spider (Ateles geoffroyi) and black howler (Alouatta pigra) monkeys across southern Mexico, using a combination of noninvasive sampling and molecular diagnostic techniques. Fecal samples were screened for filariid DNA by qPCR protocols. A total of 88 samples were examined with an overall prevalence of 26%. Filariae were slightly more common in spider monkeys compared to howler monkeys. This study constitutes the first report of the prevalence of infection of filariid nematodes in populations of wild spider monkey across southern Mexico, and the first reporting of filariae in black howler monkeys, as part of a new era of primate parasitology and the diagnostics of parasite infections in light of the everyday more affordable molecular tools.

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来源期刊
CiteScore
4.50
自引率
8.30%
发文量
103
审稿时长
4-8 weeks
期刊介绍: The objective of the American Journal of Primatology is to provide a forum for the exchange of ideas and findings among primatologists and to convey our increasing understanding of this order of animals to specialists and interested readers alike. Primatology is an unusual science in that its practitioners work in a wide variety of departments and institutions, live in countries throughout the world, and carry out a vast range of research procedures. Whether we are anthropologists, psychologists, biologists, or medical researchers, whether we live in Japan, Kenya, Brazil, or the United States, whether we conduct naturalistic observations in the field or experiments in the lab, we are united in our goal of better understanding primates. Our studies of nonhuman primates are of interest to scientists in many other disciplines ranging from entomology to sociology.
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