保守细菌 DNA 复制蛋白 DnaA 结构域 IV 的核磁共振分配。

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2024-10-04 DOI:10.1007/s12104-024-10206-1

Alexander Nguyen Abrams, Geoff Kelly, Julia Hubbard

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引用次数: 0

摘要

染色体复制是一个无处不在的重要细胞过程。在细菌中，主复制启动子 DnaA 在促进原点（oriC）开放复合物和招募螺旋酶的严格调控过程中发挥着关键作用。其 C 端结构域 IV 能特异性识别 oriC 中双链 DNA 的共识序列（称为 DnaA-boxes），从而促进 DnaA 与 oriC 的初始接合。在此，我们报告了枯草芽孢杆菌可溶性 DnaA 结构域 IV 在 pH 7.6 和 298 K 条件下的 13Cβ 和骨架 1H、15N 和 13C 化学位移赋值。

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NMR assignment of the conserved bacterial DNA replication protein DnaA domain IV

Chromosomal replication is a ubiquitous and essential cellular process. In bacteria, the master replication initiator DnaA plays a key role in promoting an open complex at the origin (oriC) and recruiting helicase in a tightly regulated process. The C-terminal domain IV specifically recognises consensus sequences of double-stranded DNA in oriC, termed DnaA-boxes, thereby facilitating the initial engagement of DnaA to oriC. Here, we report the ¹³Cβ and backbone ¹H, ¹⁵N, and ¹³C chemical shift assignments of soluble DnaA domain IV from Bacillus subtilis at pH 7.6 and 298 K.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.