Hang Gong, Shufen Yao, Yong Li, Chunyan Chen, Feng Chen and Changqun Cai
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引用次数: 0
摘要
乙型肝炎病毒（HBV）感染与肝病的进展有关。隐性乙型肝炎病毒感染（OBI）是指在乙型肝炎病毒表面抗原阴性的人体内存在可检测到的乙型肝炎病毒 DNA。然而，在未完全治愈的慢性 HBV 感染中，血清中的 HBV DNA 呈阴性，而 HBV 表面抗原仍呈阳性。因此，联合检测 HBV 表面抗原和 HBV DNA 对于准确检测和康复 HBV 感染至关重要。因此，一种基于支链 DNA 纳米结构的多重检测策略应运而生。将由四条单链DNA组装而成的支链DNA纳米结构（S4和S2段）的单链段与HBV表面抗原的适配体和DNA发夹杂交，构建了DNA纳米传感器，可实现对目标物的高特异性识别和高灵敏度荧光反应。所开发的纳米传感器对 HBV 和 HBV DNA 的检测限分别为 50 pM 和 5 nM。HBV 表面抗原和 HBV DNA 的联合检测为更全面地诊断评估 HBV 感染和康复提供了新的视角。
Combined detection of hepatitis B virus surface antigen and hepatitis B virus DNA using a DNA sensor†
Hepatitis B virus (HBV) infection is associated with the progression of liver disease. Occult HBV infection (OBI) is defined as the existence of detectable HBV DNA in HBV surface antigen negative individuals. However, HBV DNA is negative in serum while HBV surface antigen remains positive in incompletely cured chronic HBV infection. Hence, combined detection of HBV surface antigen and HBV DNA is essential for the accurate detection and rehabilitation of HBV infection. Therefore, a multiplex detection strategy based on branched DNA nanostructures was developed. The single-stranded segment of a branched DNA nanostructure (segments of S4 and S2) assembled by four single-stranded DNA was hybridized with the aptamer of HBV surface antigen and DNA hairpin to construct a DNA nanosensor, which can achieve high specificity identification and highly sensitive fluorescence responses to the targets. The detection limits of the developed nanosensor for HBV and HBV DNA are 50 pM and 5 nM, respectively. The combined detection of HBV surface antigen and HBV DNA provides a new insight for more thorough diagnostic evaluation of HBV infection and rehabilitation.
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