Longxuan Li, Jia Li, Xin Liu, Xuan Zhao, Ao Zhang, Yun Deng, Cheng Peng, Zhixing Cao, Wim Dehaen, Yuyu Fang
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Here, the first fluorescence/afterglow probe (<strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub>) for accurate and specific detection of H<sub>2</sub>O<sub>2</sub> in cells, zebrafish, and mice under Hg<sup>2+</sup>-induced oxidative stress is reported. Moreover, <strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub> in its afterglow emission enables efficient monitoring of endogenous H<sub>2</sub>O<sub>2</sub> with a higher signal-to-noise ratio (SNR) in comparison to its fluorescence signals. More importantly, by virtue of the merits of afterglow emission that can eliminate autofluorescence, thus for the first time, shortening the diagnostic window of Hg<sup>2+</sup>-induced liver injury with <strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub> via noninvasive afterglow emission tracking of H<sub>2</sub>O<sub>2</sub> is achieved, which definitely provides a new opportunity and promising tool for early diagnosis of Hg<sup>2+</sup>-induced liver injury.<h3>Environmental Implication</h3>Mercury ions (Hg<sup>2+</sup>) and mercury derivatives are a serious threat to ecosystems and human health due to their inherent toxicity, whose toxicological effects are associated with a burst of reactive oxygen species (ROS). However, the exact bioeffect role that endogenous H<sub>2</sub>O<sub>2</sub> (a featured ROS) plays in Hg<sup>2+</sup>-induced oxidative stress in a specific disease has not been well answered. Herein, the first fluorescence/afterglow probe (<strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub>) for the tracking of H<sub>2</sub>O<sub>2</sub> is reported. Shortening the diagnostic window of Hg<sup>2+</sup>-induced liver injury with <strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub> is achieved, definitely providing a promising tool for early diagnosis of Hg<sup>2+</sup>-induced liver injury.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":null,"pages":null},"PeriodicalIF":12.2000,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Shortening the early diagnostic window of Hg2+-induced liver injury with a H2O2-activated fluorescence/afterglow imaging assay\",\"authors\":\"Longxuan Li, Jia Li, Xin Liu, Xuan Zhao, Ao Zhang, Yun Deng, Cheng Peng, Zhixing Cao, Wim Dehaen, Yuyu Fang\",\"doi\":\"10.1016/j.jhazmat.2024.136059\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Mercury ions (Hg<sup>2+</sup>) and mercury derivatives are a serious threat to ecosystems and human health due to their toxicity, and their toxicological effects are associated with a burst of reactive oxygen species (ROS) due to the oxidative stress. Endogenous hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), a featured ROS in vivo, plays an irreplaceable role in a significant number of pathological processes. However, the exact bioeffect role that H<sub>2</sub>O<sub>2</sub> plays in Hg<sup>2+</sup>-induced oxidative stress in a specific disease has not been well answered. In particular, optical imaging probes for H<sub>2</sub>O<sub>2</sub> endowed with afterglow emission properties are very rare. Here, the first fluorescence/afterglow probe (<strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub>) for accurate and specific detection of H<sub>2</sub>O<sub>2</sub> in cells, zebrafish, and mice under Hg<sup>2+</sup>-induced oxidative stress is reported. Moreover, <strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub> in its afterglow emission enables efficient monitoring of endogenous H<sub>2</sub>O<sub>2</sub> with a higher signal-to-noise ratio (SNR) in comparison to its fluorescence signals. More importantly, by virtue of the merits of afterglow emission that can eliminate autofluorescence, thus for the first time, shortening the diagnostic window of Hg<sup>2+</sup>-induced liver injury with <strong>FA-H</strong><sub><strong>2</strong></sub><strong>O</strong><sub><strong>2</strong></sub> via noninvasive afterglow emission tracking of H<sub>2</sub>O<sub>2</sub> is achieved, which definitely provides a new opportunity and promising tool for early diagnosis of Hg<sup>2+</sup>-induced liver injury.<h3>Environmental Implication</h3>Mercury ions (Hg<sup>2+</sup>) and mercury derivatives are a serious threat to ecosystems and human health due to their inherent toxicity, whose toxicological effects are associated with a burst of reactive oxygen species (ROS). However, the exact bioeffect role that endogenous H<sub>2</sub>O<sub>2</sub> (a featured ROS) plays in Hg<sup>2+</sup>-induced oxidative stress in a specific disease has not been well answered. 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Shortening the early diagnostic window of Hg2+-induced liver injury with a H2O2-activated fluorescence/afterglow imaging assay
Mercury ions (Hg2+) and mercury derivatives are a serious threat to ecosystems and human health due to their toxicity, and their toxicological effects are associated with a burst of reactive oxygen species (ROS) due to the oxidative stress. Endogenous hydrogen peroxide (H2O2), a featured ROS in vivo, plays an irreplaceable role in a significant number of pathological processes. However, the exact bioeffect role that H2O2 plays in Hg2+-induced oxidative stress in a specific disease has not been well answered. In particular, optical imaging probes for H2O2 endowed with afterglow emission properties are very rare. Here, the first fluorescence/afterglow probe (FA-H2O2) for accurate and specific detection of H2O2 in cells, zebrafish, and mice under Hg2+-induced oxidative stress is reported. Moreover, FA-H2O2 in its afterglow emission enables efficient monitoring of endogenous H2O2 with a higher signal-to-noise ratio (SNR) in comparison to its fluorescence signals. More importantly, by virtue of the merits of afterglow emission that can eliminate autofluorescence, thus for the first time, shortening the diagnostic window of Hg2+-induced liver injury with FA-H2O2 via noninvasive afterglow emission tracking of H2O2 is achieved, which definitely provides a new opportunity and promising tool for early diagnosis of Hg2+-induced liver injury.
Environmental Implication
Mercury ions (Hg2+) and mercury derivatives are a serious threat to ecosystems and human health due to their inherent toxicity, whose toxicological effects are associated with a burst of reactive oxygen species (ROS). However, the exact bioeffect role that endogenous H2O2 (a featured ROS) plays in Hg2+-induced oxidative stress in a specific disease has not been well answered. Herein, the first fluorescence/afterglow probe (FA-H2O2) for the tracking of H2O2 is reported. Shortening the diagnostic window of Hg2+-induced liver injury with FA-H2O2 is achieved, definitely providing a promising tool for early diagnosis of Hg2+-induced liver injury.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.