Melanys Benitez;Pablo Zubiate;Abián B. Socorro;Ignacio R. Matías
{"title":"通过无标签检测抗花粉蛋白抗体揭示 LMR 微流控生物传感器诊断乳糜泻的能力","authors":"Melanys Benitez;Pablo Zubiate;Abián B. Socorro;Ignacio R. Matías","doi":"10.1109/LSENS.2024.3443461","DOIUrl":null,"url":null,"abstract":"Celiac disease (CD) is a chronic autoimmune disorder triggered by gluten consumption, which affects between 0.5% and 1% of the global population. Current diagnostic methods still require invasive pro-cedures, such as intestinal biopsy. Lossy mode resonance (LMR)-based sensors hold great potential for the development of reliable and user-friendly devices for diagnosing this condition. In this letter, an LMR planar microfluidic system is used to perform the label-free detection of different concentrations of antigliadin antibodies, one of the biomarkers of celiac disease, achieving a limit of detection of 1 µg/mL. The speci- ficity of the sensor to the target analyte is also proved, and the validation of the biofunctionalization process is comple- mented with an atomic force microscope analysis.","PeriodicalId":13014,"journal":{"name":"IEEE Sensors Letters","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Revealing the Capability of an LMR Microfluidic Biosensor for Celiac Disease Diagnosis via Label-Free Detection of Antigliadin Antibodies\",\"authors\":\"Melanys Benitez;Pablo Zubiate;Abián B. Socorro;Ignacio R. Matías\",\"doi\":\"10.1109/LSENS.2024.3443461\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Celiac disease (CD) is a chronic autoimmune disorder triggered by gluten consumption, which affects between 0.5% and 1% of the global population. Current diagnostic methods still require invasive pro-cedures, such as intestinal biopsy. Lossy mode resonance (LMR)-based sensors hold great potential for the development of reliable and user-friendly devices for diagnosing this condition. In this letter, an LMR planar microfluidic system is used to perform the label-free detection of different concentrations of antigliadin antibodies, one of the biomarkers of celiac disease, achieving a limit of detection of 1 µg/mL. The speci- ficity of the sensor to the target analyte is also proved, and the validation of the biofunctionalization process is comple- mented with an atomic force microscope analysis.\",\"PeriodicalId\":13014,\"journal\":{\"name\":\"IEEE Sensors Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IEEE Sensors Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://ieeexplore.ieee.org/document/10636190/\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEEE Sensors Letters","FirstCategoryId":"1085","ListUrlMain":"https://ieeexplore.ieee.org/document/10636190/","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Revealing the Capability of an LMR Microfluidic Biosensor for Celiac Disease Diagnosis via Label-Free Detection of Antigliadin Antibodies
Celiac disease (CD) is a chronic autoimmune disorder triggered by gluten consumption, which affects between 0.5% and 1% of the global population. Current diagnostic methods still require invasive pro-cedures, such as intestinal biopsy. Lossy mode resonance (LMR)-based sensors hold great potential for the development of reliable and user-friendly devices for diagnosing this condition. In this letter, an LMR planar microfluidic system is used to perform the label-free detection of different concentrations of antigliadin antibodies, one of the biomarkers of celiac disease, achieving a limit of detection of 1 µg/mL. The speci- ficity of the sensor to the target analyte is also proved, and the validation of the biofunctionalization process is comple- mented with an atomic force microscope analysis.