Kaiying Wang, Yun Wang, Lang Yang, Jinhui Li, Peihan Li, Chaojie Yang, Leili Jia, Shaofu Qiu, Hongbin Song, Peng Li
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引用次数: 0
摘要
C 组轮状病毒是全球零星病例和爆发性肠胃炎的重要病因。公共数据库中人类 C 型轮状病毒（RVC）的全基因组序列非常有限。我们进行了基因组测序，以分析中国爆发的急性肠胃炎轮状病毒。我们使用 RT-PCR 对 22 名患者的样本进行了病原体筛查，其中 6 份样本的轮状病毒检测结果呈阳性。全基因组测序分析表明，疫情株SJZ217属于G4-P[2]-I2-R2-C2-M3-A2-N2-T2-E2-H2基因型，其基因组序列与在韩国分离的Chungnam株几乎完全相同。系统进化分析表明，SJZ217 株系还与来自日本和欧洲的轮状病毒 C 株系归为一类。在 VP4 片段中观察到了重配。这些结果有助于了解 RVC 株系的遗传多样性和可能的传播。
Genomic analysis of an acute gastroenteritis outbreak caused by rotavirus C in Hebei, China.
Rotavirus group C is an important cause of sporadic cases and outbreaks of gastroenteritis worldwide. Whole-Genome sequences of human rotavirus C (RVC) in public databases are limited. We performed genome sequencing to analyze a RVC outbreak of acute gastroenteritis in China. Samples from 22 patients were screened for pathogens using RT-PCR, and six samples were positive for rotavirus. Whole-Genome sequencing analysis showed that the outbreak strain SJZ217 belongs to the G4-P[2]-I2-R2-C2-M3-A2-N2-T2-E2-H2 genotype and shares almost identical genomic sequences with Chungnam isolated in Korea. Phylogenetic analysis revealed strain SJZ217 also fell into a cluster with rotavirus C strains from Japan and Europe. Reassortment in the VP4 fragment was observed. These results helped to understand the genetic diversity and possible spread of RVC strains.
期刊介绍：
ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.
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