一次活检就能解决问题吗?通过对阔筋膜外侧和腓肠肌内侧的重复活检,重新审视人类肌肉纤维类型组成的变异性。

IF 3.3 3区 医学 Q1 PHYSIOLOGY
Journal of applied physiology Pub Date : 2024-11-01 Epub Date: 2024-10-03 DOI:10.1152/japplphysiol.00394.2024
Freek Van de Casteele, Ruud Van Thienen, Oscar Horwath, William Apró, Thibaux Van der Stede, Marcus Moberg, Eline Lievens, Wim Derave
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引用次数: 0

摘要

人体骨骼肌纤维类型组成在肌肉中差异很大,因此一次活检可能无法准确代表整块肌肉。目前还缺乏关于活检次数和使用免疫组化方法进行纤维计数的建议,也不知道这些发现是否可以推广到其他肌肉。我们评估了 40 人的外侧阔肌和内侧腓肠肌的纤维类型组成。我们从一个切口为每块肌肉采集了四个活检样本,从近端和远端针头各采集两个样本。根据另一个数据集(每个参与者 10 例阔筋膜活检样本,N=7),我们计算了活检样本子集和纤维计数与 10 例活检样本平均值相比的 95% 一致限。近端和远端以及针内样本之间 I 型纤维比例的平均绝对差异分别为:外侧肌 6.9 个百分点和 4.5 个百分点,腓肠肌内侧 5.5 个百分点和 4.4 个百分点。当分析至少三份活检样本中的 200 根纤维时,95% 的一致性界限缩小到 ±10 个百分点,纤维数量越多,一致性界限越小。大腿外侧肌和腓肠肌内侧的 I 型纤维比例显示出中等程度的正相关(r²=0.22;p=0.006;每块肌肉三至四个样本中每个样本至少有 200 根纤维)。总之,要在±10 个百分点的范围内估算出阔筋膜外侧肌纤维类型组成,需要进行三次活检并至少计数 200 根纤维。即使使用这些标准,研究人员在将肌肉纤维类型比例从一块肌肉推断到另一块肌肉时也应谨慎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Does one biopsy cut it? Revisiting human muscle fiber type composition variability using repeated biopsies in the vastus lateralis and gastrocnemius medialis.

Human skeletal muscle fiber type composition varies greatly along the muscle, so one biopsy may not accurately represent the whole muscle. Recommendations on the number of biopsies and fiber counts using immunohistochemistry and whether these findings can be extrapolated to other muscles are lacking. We assessed fiber type composition in the vastus lateralis and gastrocnemius medialis muscles of 40 individuals. Per muscle, we took four biopsy samples from one incision, collecting two samples each from a proximally and distally directed needle. Based on another dataset involving 10 vastus lateralis biopsies per participant (n = 7), we calculated 95% limits of agreement for subsets of biopsies and fiber counts compared with the 10-biopsy average. Average absolute differences in type I fiber proportions between proximal and distal, and between within-needle samples were 6.9 and 4.5 percentage points in the vastus lateralis, and 5.5 and 4.4 percentage points in the gastrocnemius medialis, respectively. The 95% limits of agreement narrowed to ±10 percentage points when 200 fibers from at least three biopsies were analyzed, with minimal improvements with greater fiber counts. Type I fiber proportions in the vastus lateralis and gastrocnemius medialis showed a moderate positive association (r2 = 0.22; P = 0.006; at least 200 fibers in each of three to four samples per muscle). In conclusion, three biopsies with a minimum of 200 counted fibers are required to estimate the vastus lateralis fiber type composition within ±10 percentage points. Even when using these standards, researchers should be cautious when extrapolating muscle fiber type proportions from one muscle to another.NEW & NOTEWORTHY Fiber type composition is equally variable in muscle biopsy samples taken from one incision as from multiple incisions. Hence, we propose two biopsies from a single incision-needles directed proximally and distally, and each rotated 180° for cutting a second sample-as a more feasible, less invasive alternative to three biopsies from as many incisions. In addition, we identified the gastrocnemius medialis as a slow-twitch muscle with an average of 64.7% slow fibers.

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来源期刊
CiteScore
6.00
自引率
9.10%
发文量
296
审稿时长
2-4 weeks
期刊介绍: The Journal of Applied Physiology publishes the highest quality original research and reviews that examine novel adaptive and integrative physiological mechanisms in humans and animals that advance the field. The journal encourages the submission of manuscripts that examine the acute and adaptive responses of various organs, tissues, cells and/or molecular pathways to environmental, physiological and/or pathophysiological stressors. As an applied physiology journal, topics of interest are not limited to a particular organ system. The journal, therefore, considers a wide array of integrative and translational research topics examining the mechanisms involved in disease processes and mitigation strategies, as well as the promotion of health and well-being throughout the lifespan. Priority is given to manuscripts that provide mechanistic insight deemed to exert an impact on the field.
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