Cassie G Ackerley, S Abigail Smith, Phillip M Murray, Praveen K Amancha, Vanessa E Van Doren, Gregory K Tharp, Robert A Arthur, Rama R Amara, Yi-Juan Hu, Colleen F Kelley
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引用次数: 0
摘要
微生物组和肠道粘膜驻留免疫细胞之间的交叉对话在调节对病原体的免疫反应(包括对艾滋病病毒感染的反应)中起着关键作用。然而,与年长的成年男男性行为者(ASM)相比,受 HIV 影响尤为严重的年轻男男性行为者(YMSM)之间的这些相互作用有何不同,目前还不十分清楚。对微生物组和直肠转录组之间关联的广泛分析显示,有 10 个微生物科/属与免疫基因通路相关。具体来说,与 AMSM 相比,YMSM 直肠转录组的特点是 T 细胞活化/分化途径和多个细胞因子家族信号的上调。YMSM的微生物组富含病原菌属,包括钩端螺旋体,而钩端螺旋体与对抗病毒免疫非常重要的I型干扰素通路呈正相关。这些研究结果表明,YMSM 具有独特的免疫表型和直肠微环境,有助于进一步评估影响直肠 HIV 传播的生物因素。
Integrated analysis of rectal mucosal microbiome and transcriptome reveals a distinct microenvironment among young MSM.
Crosstalk between the microbiome and gut mucosa-resident immune cells plays a pivotal role in modulating immune responses to pathogens, including responses to HIV infection. However, how these interactions may differ between young men who have sex with men (YMSM) disproportionately impacted by HIV, as compared with older adult MSM (AMSM), is not well understood. A broad analysis of associations between the microbiome and rectal transcriptome revealed 10 microbial families/genera correlated with immunologic gene pathways. Specifically, the rectal transcriptome of YMSM was characterized by upregulation of T cell activation/differentiation pathways and signaling from multiple cytokine families compared with AMSM. The microbiome of YMSM was enriched with pathogenic genera, including Peptostreptococcus, shown to be positively correlated with type I IFN pathways important for antiviral immunity. These findings demonstrate that YMSM have a unique immune phenotype and rectal microenvironment and support further evaluation of biological factors that influence rectal HIV transmission.
期刊介绍:
JCI Insight is a Gold Open Access journal with a 2022 Impact Factor of 8.0. It publishes high-quality studies in various biomedical specialties, such as autoimmunity, gastroenterology, immunology, metabolism, nephrology, neuroscience, oncology, pulmonology, and vascular biology. The journal focuses on clinically relevant basic and translational research that contributes to the understanding of disease biology and treatment. JCI Insight is self-published by the American Society for Clinical Investigation (ASCI), a nonprofit honor organization of physician-scientists founded in 1908, and it helps fulfill the ASCI's mission to advance medical science through the publication of clinically relevant research reports.