Louisa Lu, Ali Fauci, Quintin R Richardson, Hamidah Mahmud, John M Nesemann, Solomon Aragie, Dagnachew Hailu, Adane Dagnew, Ambahun Chernet, Zerihun Tadesse, Taye Zeru, Isabel J B Thompson, Dionna M Wittberg, Scott D Nash, Thomas M Lietman, Jeremy D Keenan
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引用次数: 0
摘要
本研究旨在探讨定量聚合酶链式反应（qPCR）测定的沙眼衣原体细菌量与沙眼炎症临床严重程度之间的相关性。研究人员为埃塞俄比亚农村社区的沙眼患者拍摄了照片，并采集了跗骨结膜上部的拭子。结膜拭子经过 PCR 检测处理，可提供眼部衣原体载量的定量结果。根据滤泡性结膜炎和乳头状结膜炎的临床严重程度，对 125 张结膜照片进行了从轻到重的排序。在滤泡性结膜炎和乳头状结膜炎中，沙眼炎症程度越严重，衣原体载量越高（Spearman's ρ = 0.43; P.
Correlation of Clinical Severity of Trachomatous Inflammation and Chlamydia trachomatis Bacterial Load.
The purpose of this study was to investigate the correlation between bacterial load of Chlamydia trachomatis as measured from quantitative polymerase chain reaction (qPCR) and the relative clinical severity of trachomatous inflammation. Individuals with trachoma from rural communities in Ethiopia had photographs taken as well as swabs obtained of the upper tarsal conjunctivas. Conjunctival swabs were processed with PCR assay, which provided quantitative results of ocular chlamydial load. A series of 125 conjunctival photographs were ranked from least to most severe according to clinical severity for follicular and papillary conjunctivitis. Higher intensity rankings of trachomatous inflammation were associated with higher chlamydial load for both follicular inflammation (Spearman's ρ = 0.43; P <0.001) and papillary inflammation (Spearman's ρ = 0.50; P <0.001). Rankings of trachomatous inflammation may be a clinically meaningful indicator of trachoma.
期刊介绍：
The American Journal of Tropical Medicine and Hygiene, established in 1921, is published monthly by the American Society of Tropical Medicine and Hygiene. It is among the top-ranked tropical medicine journals in the world publishing original scientific articles and the latest science covering new research with an emphasis on population, clinical and laboratory science and the application of technology in the fields of tropical medicine, parasitology, immunology, infectious diseases, epidemiology, basic and molecular biology, virology and international medicine.
The Journal publishes unsolicited peer-reviewed manuscripts, review articles, short reports, images in Clinical Tropical Medicine, case studies, reports on the efficacy of new drugs and methods of treatment, prevention and control methodologies,new testing methods and equipment, book reports and Letters to the Editor. Topics range from applied epidemiology in such relevant areas as AIDS to the molecular biology of vaccine development.
The Journal is of interest to epidemiologists, parasitologists, virologists, clinicians, entomologists and public health officials who are concerned with health issues of the tropics, developing nations and emerging infectious diseases. Major granting institutions including philanthropic and governmental institutions active in the public health field, and medical and scientific libraries throughout the world purchase the Journal.
Two or more supplements to the Journal on topics of special interest are published annually. These supplements represent comprehensive and multidisciplinary discussions of issues of concern to tropical disease specialists and health issues of developing countries
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