Jie Gao, Na Zhang, Guohui Liu, Jinjun Tian, Mengyao Chen, Ying Wang, Ye Xing, Ying Zhang, Chenyang Zhao, Xiaohuan Mu, Yanwen Yu, Hongbin Niu, Jiankun Li, Jihua Tang, Mingyue Gou
{"title":"ZmSKI3 介导的 RNA 衰变和转录后基因沉默对玉米生长和免疫的调控。","authors":"Jie Gao, Na Zhang, Guohui Liu, Jinjun Tian, Mengyao Chen, Ying Wang, Ye Xing, Ying Zhang, Chenyang Zhao, Xiaohuan Mu, Yanwen Yu, Hongbin Niu, Jiankun Li, Jihua Tang, Mingyue Gou","doi":"10.1111/jipb.13780","DOIUrl":null,"url":null,"abstract":"<p>Disease resistance is often associated with compromised plant growth and yield due to defense-growth tradeoffs. However, key components and mechanisms underlying the defense-growth tradeoffs are rarely explored in maize. In this study, we find that ZmSKI3, a putative subunit of the SUPERKILLER (SKI) complex that mediates the 3′-5′ degradation of RNA, regulates both plant development and disease resistance in maize. The <i>Zmski3</i> mutants showed retarded plant growth and constitutively activated defense responses, while the <i>ZmSKI3</i> overexpression lines are more susceptible to <i>Curvularia lunata</i> and <i>Bipolaris maydis</i>. Consistently, the expression of defense-related genes was generally up-regulated, while expressions of growth-related genes were mostly down-regulated in leaves of the <i>Zmski3-1</i> mutant compared to that of wild type. In addition, 223 differentially expressed genes that are up-regulated in <i>Zmski3-1</i> mutant but down-regulated in the <i>ZmSKI3</i> overexpression line are identified as potential target genes of ZmSKI3. Moreover, small interfering RNAs targeting the transcripts of the defense- and growth-related genes are differentially accumulated, likely to combat the increase of defense-related transcripts but decrease of growth-related transcripts in <i>Zmski3-1</i> mutant. Taken together, our study indicates that plant growth and immunity could be regulated by both ZmSKI3-mediated RNA decay and post-transcriptional gene silencing in maize.</p>","PeriodicalId":195,"journal":{"name":"Journal of Integrative Plant Biology","volume":"66 11","pages":"2561-2577"},"PeriodicalIF":9.3000,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jipb.13780","citationCount":"0","resultStr":"{\"title\":\"Regulation of maize growth and immunity by ZmSKI3-mediated RNA decay and post-transcriptional gene silencing\",\"authors\":\"Jie Gao, Na Zhang, Guohui Liu, Jinjun Tian, Mengyao Chen, Ying Wang, Ye Xing, Ying Zhang, Chenyang Zhao, Xiaohuan Mu, Yanwen Yu, Hongbin Niu, Jiankun Li, Jihua Tang, Mingyue Gou\",\"doi\":\"10.1111/jipb.13780\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Disease resistance is often associated with compromised plant growth and yield due to defense-growth tradeoffs. However, key components and mechanisms underlying the defense-growth tradeoffs are rarely explored in maize. In this study, we find that ZmSKI3, a putative subunit of the SUPERKILLER (SKI) complex that mediates the 3′-5′ degradation of RNA, regulates both plant development and disease resistance in maize. The <i>Zmski3</i> mutants showed retarded plant growth and constitutively activated defense responses, while the <i>ZmSKI3</i> overexpression lines are more susceptible to <i>Curvularia lunata</i> and <i>Bipolaris maydis</i>. Consistently, the expression of defense-related genes was generally up-regulated, while expressions of growth-related genes were mostly down-regulated in leaves of the <i>Zmski3-1</i> mutant compared to that of wild type. In addition, 223 differentially expressed genes that are up-regulated in <i>Zmski3-1</i> mutant but down-regulated in the <i>ZmSKI3</i> overexpression line are identified as potential target genes of ZmSKI3. Moreover, small interfering RNAs targeting the transcripts of the defense- and growth-related genes are differentially accumulated, likely to combat the increase of defense-related transcripts but decrease of growth-related transcripts in <i>Zmski3-1</i> mutant. 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Regulation of maize growth and immunity by ZmSKI3-mediated RNA decay and post-transcriptional gene silencing
Disease resistance is often associated with compromised plant growth and yield due to defense-growth tradeoffs. However, key components and mechanisms underlying the defense-growth tradeoffs are rarely explored in maize. In this study, we find that ZmSKI3, a putative subunit of the SUPERKILLER (SKI) complex that mediates the 3′-5′ degradation of RNA, regulates both plant development and disease resistance in maize. The Zmski3 mutants showed retarded plant growth and constitutively activated defense responses, while the ZmSKI3 overexpression lines are more susceptible to Curvularia lunata and Bipolaris maydis. Consistently, the expression of defense-related genes was generally up-regulated, while expressions of growth-related genes were mostly down-regulated in leaves of the Zmski3-1 mutant compared to that of wild type. In addition, 223 differentially expressed genes that are up-regulated in Zmski3-1 mutant but down-regulated in the ZmSKI3 overexpression line are identified as potential target genes of ZmSKI3. Moreover, small interfering RNAs targeting the transcripts of the defense- and growth-related genes are differentially accumulated, likely to combat the increase of defense-related transcripts but decrease of growth-related transcripts in Zmski3-1 mutant. Taken together, our study indicates that plant growth and immunity could be regulated by both ZmSKI3-mediated RNA decay and post-transcriptional gene silencing in maize.
期刊介绍:
Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.